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Alpha 2 giardin is an assemblage A-specific protein of human infective Giardia duodenalis

Published online by Cambridge University Press:  22 October 2008

R. F. L. STEUART ,
R. O'HANDLEY ,
R. J. LIPSCOMBE ,
R. A. LOCK  and
R. C. A. THOMPSON
R. F. L. STEUART*
Affiliation:
WHO Collaborating Centre for the Molecular Epidemiology of Parasitic Infection and Environmental Biotechnology CRC, School of Veterinary and Biomedical Sciences, Murdoch University, Murdoch, 6150, Western Australia
R. O'HANDLEY
Affiliation:
WHO Collaborating Centre for the Molecular Epidemiology of Parasitic Infection and Environmental Biotechnology CRC, School of Veterinary and Biomedical Sciences, Murdoch University, Murdoch, 6150, Western Australia
R. J. LIPSCOMBE
Affiliation:
Proteomics International Pty Ltd, PO Box 6064, East Perth 6892, Western Australia
R. A. LOCK
Affiliation:
Proteomics International Pty Ltd, PO Box 6064, East Perth 6892, Western Australia
R. C. A. THOMPSON
Affiliation:
WHO Collaborating Centre for the Molecular Epidemiology of Parasitic Infection and Environmental Biotechnology CRC, School of Veterinary and Biomedical Sciences, Murdoch University, Murdoch, 6150, Western Australia
*
*Corresponding author: School of Veterinary and Biomedical Science, Murdoch University, Murdoch, 6150, Western Australia. Tel: +61 8 9360 2690. E-mail: [email protected]
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Summary

Of the 7 genetic assemblages of the parasite Giardia duodenalis only 2 (A and B) are known to cause infections in humans. These assemblages have been characterized in detail at the genomic level but few studies have examined differences in the proteins expressed. Employing one and two-dimensional PAGE we have identified an assemblage A-specific protein of human infective G. duodenalis; alpha 2 giardin. The protein difference was evident using both electrophoretic techniques. Alpha 2 giardin is known to be a structural protein and associates with the caudal flagella and the plasma membrane; however, its exact function is unknown. Although several proteins unique to assemblage B were also observed, we were unable to identify these proteins due to a lack of genomic data available for assemblage B isolates. Together, these proteins represent distinct phenotypic differences between the human infective assemblages of G. duodenalis and support the need to revise the taxonomy of this parasite.

Keywords

Giardiacomparative proteomicsinter-assemblage variationalpha 2 giardin
Type
Research Article
Information
Parasitology , Volume 135 , Issue 14 , December 2008 , pp. 1621 - 1627
Copyright
Copyright © 2008 Cambridge University Press

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