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Allele-specific, nested, one tube PCR: application to Pfmdr1 polymorphisms in Plasmodium falciparum

Published online by Cambridge University Press:  01 July 1999

I. S. ADAGU  and
D. C. WARHURST
I. S. ADAGU
Affiliation:
Department of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, Keppel Street, London WC1E 7HT, UK
D. C. WARHURST
Affiliation:
Department of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, Keppel Street, London WC1E 7HT, UK
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Abstract

An allele-specific, one tube PCR for the sensitive and reliable detection of point mutations in Plasmodium falciparum DNA is described. Design of specific internal primers and optimization of the PCR is simple, and the procedure is robust and sensitive. Single nucleotide polymorphisms at codons 184, 1034, 1042 and 1246 of the P. falciparum multidrug resistance gene Pfmdr1, were examined in 6 laboratory isolates, to validate the technique.

Keywords

allele-specific PCRone tube semi-nested reactionPfmdr1Plasmodium falciparummalaria
Type
Research Article
Information
Parasitology , Volume 119 , Issue 1 , July 1999 , pp. 1 - 6
Copyright
1999 Cambridge University Press

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