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Adhesion of Pasteuria penetrans to the cuticle of root-knot nematodes (Meloidogyne spp.) inhibited by fibronectin: a study of electrostatic and hydrophobic interactions

Published online by Cambridge University Press:  06 April 2009

K. G. Davies ,
P. Afolabi  and
P. O'Shea
K. G. Davies
Affiliation:
Entomology and Nematology Department, AFRC-Institute of Arable Crops Research, Rothamsted Experimental Station, Harpenden, Hertfordshire AL5 2JQ
P. Afolabi
Affiliation:
Department of Biological and Chemical Sciences, University of Essex, Wivenhoe Park, Colchester, Essex CO4 3SQ
P. O'Shea*
Affiliation:
Department of Biological and Chemical Sciences, University of Essex, Wivenhoe Park, Colchester, Essex CO4 3SQ
*
* Corresponding author. Fax: + 44 1206 872592. E-mail: [email protected].
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Summary

Pasteuria penetrans is a bacterium with the potential to control plant-parasitic nematode populations; the mechanism of spore adhesion, however, is poorly understood. Attachment assays were performed in media supplemented with various concentrations of fibronectin and in the presence and absence of KSCN which modulates hydrophobic interactions. A reduction in the strength of the hydrophobic effect prevented spores from binding to the cuticle as did 20 μg/ml fibronectin. It was also shown directly utilizing a newly-developed technique which utilizes 3-hexadecanoyl-7-hydro-coumarin as an indicator of the fibronectin binding to the spore surface that the presence of KSCN prohibited binding. This effect was interpreted to indicate that the reduction of binding was the direct result of the influence of hydrophobic interactions between the fibronectin and the spore surface. Western blot analysis of cuticle extracts of Meloidogyne incognita and Caenorhabditis elegans revealed small amounts of fibronectin to be present. Fibronectin, or a similar receptor, present in the cuticle could be responsible for the adhesion of P. penetrans by hydrophobic interactions.

Keywords

bacterial adhesionfibronectinextracellular matrixNematodaMeloidogynePasteuriacuticlebiological controlelectrostatic potentialhydrophobic surface
Type
Research Article
Information
Parasitology , Volume 112 , Issue 6 , June 1996 , pp. 553 - 559
Copyright
Copyright © Cambridge University Press 1996

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