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Activities of glycogen phosphorylase, alanine aminotransferase and aspartate aminotransferase in adult worms of Litomosoides carinii recovered from pyridoxine deficient cotton rats (Sigmodon hispidus)

Published online by Cambridge University Press:  26 March 2010

M. A. Beg ,
J. L. Fistein ,
G. A. Ingram  and
D. M. Storey
M. A. Beg
Affiliation:
Science Research Institute and Department of Biological Sciences, University of Salford, Salford M5 4WT
J. L. Fistein
Affiliation:
St Mary's Hospital Medical School, London W2 1UF
G. A. Ingram
Affiliation:
Science Research Institute and Department of Biological Sciences, University of Salford, Salford M5 4WT
D. M. Storey
Affiliation:
Science Research Institute and Department of Biological Sciences, University of Salford, Salford M5 4WT
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Summary

This paper demonstrates that the activities of glycogen phosphorylase (GP), alanine aminotransferase (ALT) and aspartate aminotransferase (AST) are reduced in adult worms of the filarial nematode Litomosoides carinii recovered from pyri-doxine-deficient cotton rats when compared to worms recovered from pyridoxine-sufficient controls. GP, ALT and AST activities were determined in adult worms L. carinii recovered from cotton rat hosts over a 20-week experimental period. Activities of GP, ALT and AST in the parasite showed a direct correlation with the dietary pyridoxine intake of their host. Throughout the experiment, enzyme activities were significantly lower (P < 0·001) in worms from rats fed a pyridoxine-free diet ad libitum that in worms from rats fed either a stock colony diet, a pyridoxine-free diet ad libitum with daily supplementation of 100 μg pyridoxine or limited amounts of pyridoxine-free diet with daily supplementation of 100 μg pyridoxine. The lower than normal activity of GP, ALT, AST and other enzymes dependent on the biologically active derivative of pyridoxine, the coenzyme pyridoxal-5-phosphate (PLP), interferes with the protein, carbohydrate and lipid metabolism of L. carinii and may in part cause the reduced establishment, development and growth of the parasite in pyridoxine-deficient hosts.

Keywords

Litomosoides cariniifilariasiscotton ratsSigmodon hispidusvitamin B6pyridoxineglycogen phosphorylasealanine aminotransferaseaspartate aminotransferasepyridoxine-5-phosphate
Type
Research Article
Information
Parasitology , Volume 112 , Issue 2 , February 1996 , pp. 227 - 232
Copyright
Copyright © Cambridge University Press 1996

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