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Action of praziquantel on Grillotia erinaceus metacestodes (Cestoda: Trypanorhyncha) in vitro

Published online by Cambridge University Press:  06 April 2009

S. M. Ward ,
J. M. Allen  and
G. McKeer
S. M. Ward
Affiliation:
Department of Biology, University of Ulster, Shore Road, Newtownabbey, Co. Antrim BT 37 0QB, Ireland
J. M. Allen*
Affiliation:
Department of Biology, University of Ulster, Shore Road, Newtownabbey, Co. Antrim BT 37 0QB, Ireland
G. McKeer
Affiliation:
Department of Biology, University of Ulster, Shore Road, Newtownabbey, Co. Antrim BT 37 0QB, Ireland
*
To whom corresspondence should be addressed
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Summary

Isometric tension recordings and the single sucrose-gap technique were used to record mechanical and electrical activity of somatic muscle from G. erinaceus metacestodes in vitro. Praziquantel (≥ 10−6 M) raised baseline tension and largely abolished spontaneous fluctuations in tension. The effect of the drug was critically dependent on the presence and availability of calcium in the bathing medium. In calcium-free solution or in the presence of manganese or lanthanum (10 mm) praziquantel failed to induce a rise in baseline tension. Electrical recordings of somatic muscle activity showed the action of praziquantel to be accompanied by membrane depolarization and loss of slow depolarizations and spikes. Tegumental membrane potential, as measured by microelectrodes, was not affected by the drug. These results suggest that in vitro praziquantel causes contraction of the somatic muscle of G. erinaceus by membrane depolarization and influx of extracellular calcium.

Type
Research Article
Information
Parasitology , Volume 93 , Issue 1 , August 1986 , pp. 133 - 142
Copyright
Copyright © Cambridge University Press 1986

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References

Andrews, P. & Thomas, H. (1979). The effects of praziquantel on Hymenolepis diminuta in vitro. Journal of Tropical Medicine and Parasitology 30, 391–400.Google ScholarPubMed
Becker, B., Mehlhorn, H., Andrews, P. & Thomas, H. (1981). Ultrastructural investigations on the effect of praziquantel on the tegument of five species of cestodes. Zeitschrift für Parasitenkunde 64, 257–69.CrossRefGoogle ScholarPubMed
Becker, B., Mehlhorn, H., Andrews, P., Thomas, H. & Eckert, J. (1981). Light and electron microscopic studies on the effect of praziquantel on Schistosoma mansoni, Dicrocoelium dendriticum and Fasciola hepatica (Trematoda) in vitro. Zeitschrift für Parasitenkunde 63, 113–28.CrossRefGoogle Scholar
Bricker, C. S., Pax, R. A. & Bennett, J. L. (1982). Microelectrode studies of the tegument and sub-tegumental compartments of male Schistosoma mansoni: anatomical location of sources of electrical potentials. Parasitology 85, 149–61.CrossRefGoogle ScholarPubMed
Conder, G., Marchiondo, A. & Anderson, F. (1981). Effect of praziquantel on adult Echinococcus granulosus in vitro: scanning electron microscopy. Zeitschrift für Parasitenkunde 66, 191–9.CrossRefGoogle ScholarPubMed
Endo, M. (1977). Calcium release from the sarcoplasmic reticulum. Physiological Reviews 57, 71–108.CrossRefGoogle ScholarPubMed
Fetterer, R. H., Pax, R. A. & Bennett, J. L. (1980). Praziquantel, potassium and 2,4-dinitrophenol: analysis of their action on the musculature of Schistosoma mansoni. European Journal of Pharmacology 64, 31–8.CrossRefGoogle ScholarPubMed
Frankenhaeuser, B., & Hodgkin, A. L. (1957). The action of calcium on the electrical properties of squid axons. Journal of Physiology 137, 218–44.CrossRefGoogle ScholarPubMed
Gönnert, R. & Andrews, P. (1977). Praziquantel, a new broad spectrum antischistosomal agent. Zeitschrift für Parasitenkunde 52, 129–50.CrossRefGoogle Scholar
Hodgkin, A. L. & Horowicz, P. (1959). The influence of potassium and chloride ions on the membrane potential of single muscle fibres. Journal of Physiology 148, 127–60.CrossRefGoogle ScholarPubMed
Kuriyama, H. (1981). Excitation–contraction coupling in various visceral smooth muscles. In Smooth Muscle: an Assessment of Current Knowledge (ed. E., Bülbring, Brading, A. F., Jones, A. W. and Tomita, T.), pp. 171–197. London: Edward Arnold Ltd.Google Scholar
Mussie, E. W., Vande Waa, J., Pax, R. A., Fetterer, R. H. & Bennett, J. L. (1982). Schistosoma mansoni: Ca2+ efflux and effects of Ca2+-free media on responses of the adult male musculature to praziquantel and other agents inducing contraction. Experimental Parasitology 53, 270–8.CrossRefGoogle Scholar
Pax, R. A., Bennett, J. L. & Fetterer, R. H. (1978). A benzodiazepine derivative and praziquantel: effects on musculature of Schistosoma mansoni and Schistosoma japonicum. Naunyn-Schmiedeberg's Archiv für Pharmakologie 34, 309–15.CrossRefGoogle Scholar
Prichard, R. K., Bachmann, R., Hutchinson, G. W. & Köhler, P. (1982). The effect of praziquantel on Ca2+ in Hymenolepis diminuta. Molecular and Biochemical Parasitology 5, 297–308.CrossRefGoogle ScholarPubMed
Shaw, M. K. & Erasmus, D. A. (1983). Schistosoma mansoni: the effects of a subcurative dose of praziquantel on the ultrastructure of worms in vivo. Zeitschrift für Parasitenkunde 69, 73–90.CrossRefGoogle ScholarPubMed
Thomas, H. & Gönnert, R. (1977). The efficacy of praziquantel against cestodes in animals. Zeitschrift für Parasitenkunde 52, 117–27.CrossRefGoogle ScholarPubMed
Thompson, C. S. & Mettrick, D. F. (1984). Neuromuscular physiology of Hymenolepis diminuta and H. microstoma (Cestoda). Parasitology 89, 567–78.CrossRefGoogle Scholar
Ward, S. M., Allen, J. M. & McKerr, G. (1986). Neuromuscular physiology of Grillotia erinaceus metacestodes (Cestoda: Trypanorhyncha) in vitro. Parasitology 93, 121–32.CrossRefGoogle Scholar
Webbe, G. & James, C. (1977). A comparison of the susceptibility to praziquantel of Schistosoma haematobium, Schistosoma japonicum, Schistosoma mansoni, Schistosoma intercalatum and Schistosoma mattheei in hamsters. Zeitschrift für Parasitenkunde 52, 169–77.CrossRefGoogle ScholarPubMed