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Acquired immunity to Schistosoma mansoni in the rat: measurement of immunity by the lung recovery technique

Published online by Cambridge University Press:  06 April 2009

Hilda Perez ,
J. A. Clegg  and
S. R. Smithers
Hilda Perez
Affiliation:
Division of Parasitology, National Institute for Medical Research, Mill Hill, London NW7 1AA
J. A. Clegg
Affiliation:
Division of Parasitology, National Institute for Medical Research, Mill Hill, London NW7 1AA
S. R. Smithers
Affiliation:
Division of Parasitology, National Institute for Medical Research, Mill Hill, London NW7 1AA
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Extract

Acquired immunity to Schistosoma mansoni in the rat can be assayed by the recovery of a proportion of the schistosomula of a challenge infection from the lungs 5 days after the challenge has been given. The recoveries from immune rats, which are significantly less than those from control animals, demonstrate that a proportion of a challenge infection is killed in the lungs or at an earlier point in the pathway of migration.

Immunity in the Sprague–Dawley rat can first be shown by the lung recovery technique 3 weeks after an immunizing exposure of 500 cercariae. Immunity reaches a peak between weeks 6 and 7 but then declines to zero by week 12. The PVG inbred Hooded rat shows a similar but delayed development and decline of immunity. When the lung recovery technique shows immunity to be declining, hepatic perfusion demonstrates that immunity to reinfection is partially retained, indicating that at this stage the challenge is killed after the first 5 days.

Re-exposure of rats in which immunity has declined induces an anamnestic lung recovery response. There appears to be a relationship between spontaneous cure and the decline of immunity. A factor in the serum of infected rats which kills young schistosomula in culture develops in parallel with immunity, but high titres of this factor are maintained during the decline of immunity.

Type
Research Article
Information
Parasitology , Volume 69 , Issue 3 , December 1974 , pp. 349 - 359
Copyright
Copyright © Cambridge University Press 1974

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References

REFERENCES

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