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Whole-genome amplification: a useful approach to characterize new genes in unculturable protozoan parasites such as Bonamia exitiosa

Published online by Cambridge University Press:  18 August 2015

MARIA PRADO-ALVAREZ ,
YANN COURALEAU ,
BRUNO CHOLLET ,
DELPHINE TOURBIEZ  and
ISABELLE ARZUL
MARIA PRADO-ALVAREZ
Affiliation:
Laboratoire de Génétique et Pathologie des Mollusques Marins, IFREMER, Avenue de Mus de Loup, 17390 La Tremblade, France
YANN COURALEAU
Affiliation:
Laboratoire de Génétique et Pathologie des Mollusques Marins, IFREMER, Avenue de Mus de Loup, 17390 La Tremblade, France
BRUNO CHOLLET
Affiliation:
Laboratoire de Génétique et Pathologie des Mollusques Marins, IFREMER, Avenue de Mus de Loup, 17390 La Tremblade, France
DELPHINE TOURBIEZ
Affiliation:
Laboratoire de Génétique et Pathologie des Mollusques Marins, IFREMER, Avenue de Mus de Loup, 17390 La Tremblade, France
ISABELLE ARZUL*
Affiliation:
Laboratoire de Génétique et Pathologie des Mollusques Marins, IFREMER, Avenue de Mus de Loup, 17390 La Tremblade, France
*
* Corresponding author. Laboratoire de Génétique et Pathologie des Mollusques Marins, IFREMER, Avenue de Mus de Loup, 17390 La Tremblade, France. E-mail: [email protected]
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Summary

Bonamia exitiosa is an intracellular parasite (Haplosporidia) that has been associated with mass mortalities in oyster populations in the Southern hemisphere. This parasite was recently detected in the Northern hemisphere including Europe. Some representatives of the Bonamia genus have not been well categorized yet due to the lack of genomic information. In the present work, we have applied Whole-Genome Amplification (WGA) technique in order to characterize the actin gene in the unculturable protozoan B. exitiosa. This is the first protein coding gene described in this species. Molecular analysis revealed that B. exitiosa actin is more similar to Bonamia ostreae actin gene-1. Actin phylogeny placed the Bonamia sp. infected oysters in the same clade where the herein described B. exitiosa actin resolved, offering novel information about the classification of the genus. Our results showed that WGA methodology is a promising and valuable technique to be applied to unculturable protozoans whose genomic material is limited.

Keywords

Bonamia exitiosaBonamia ostreaeactinHaplosporidiaWhole-Genome Amplification
Type
Research Article
Information
Parasitology , Volume 142 , Issue 12 , October 2015 , pp. 1523 - 1534
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Copyright
Copyright © Cambridge University Press 2015 

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