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Viability of Ascaris suum eggs in stored raw and separated liquid slurry

Published online by Cambridge University Press:  06 November 2012

KIRAN KUMAR KATAKAM ,
ALLAN ROEPSTORFF ,
OLGA POPOVIC ,
NIELS C. KYVSGAARD ,
STIG MILAN THAMSBORG  and
ANDERS DALSGAARD
KIRAN KUMAR KATAKAM
Affiliation:
Department of Veterinary Disease Biology, Faculty of Health and Medical Sciences, University of Copenhagen, Denmark
ALLAN ROEPSTORFF
Affiliation:
Department of Veterinary Disease Biology, Faculty of Health and Medical Sciences, University of Copenhagen, Denmark
OLGA POPOVIC
Affiliation:
Department of Agriculture and Ecology, Faculty of Science, University of Copenhagen, Denmark
NIELS C. KYVSGAARD
Affiliation:
Department of Large Animal Sciences, Faculty of Health and Medical Sciences, University of Copenhagen, Denmark
STIG MILAN THAMSBORG
Affiliation:
Department of Veterinary Disease Biology, Faculty of Health and Medical Sciences, University of Copenhagen, Denmark
ANDERS DALSGAARD*
Affiliation:
Department of Veterinary Disease Biology, Faculty of Health and Medical Sciences, University of Copenhagen, Denmark
*
*Corresponding author: Department of Veterinary Disease Biology, Faculty of Health and Medical Sciences, University of Copenhagen, Groennegaardsvej 15, DK-1870 Frederiksberg C, Denmark. Tel: +45 35332720. Fax: +45 35332757. E-mail: [email protected]
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Summary

Separation of pig slurry into solid and liquid fractions is gaining importance as a way to manage increasing volumes of slurry. In contrast to solid manure and slurry, little is known about pathogen survival in separated liquid slurry. The viability of Ascaris suum eggs, a conservative indicator of fecal pollution, and its association with ammonia was investigated in separated liquid slurry in comparison with raw slurry. For this purpose nylon bags with 6000 eggs each were placed in 1 litre bottles containing one of the two fractions for 308 days at 5 °C or 25 °C. Initial analysis of helminth eggs in the separated liquid slurry revealed 47 Ascaris eggs per gramme. At 25 °C, egg viability declined to zero with a similar trend in both raw slurry and the separated liquid slurry by day 308, a time when at 5 °C 88% and 42% of the eggs were still viable in separated liquid slurry and raw slurry, respectively. The poorer survival at 25 °C was correlated with high ammonia contents in the range of 7·9–22·4 mm in raw slurry and 7·3–23·2 mm in liquid slurry compared to 3·2–9·5 mm in raw slurry and 2·6–9·5 mm in liquid slurry stored at 5 °C. The study demonstrates that at 5 °C, A. suum eggs have a higher viability in separated liquid slurry as compared to raw slurry. The hygiene aspect of this needs to be further investigated when separated liquid slurry is used to fertilize pastures or crops.

Keywords

raw slurryseparated liquid slurryviabilityAscaris suum eggstemperatureammonia
Type
Research Article
Information
Parasitology , Volume 140 , Issue 3 , March 2013 , pp. 378 - 384
Copyright
Copyright © Cambridge University Press 2012

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