Hostname: page-component-586b7cd67f-2plfb Total loading time: 0 Render date: 2024-11-26T11:15:55.221Z Has data issue: false hasContentIssue false
  • English
  • Français

Vaccination against Trichinella spiralis in mice using antigens from different isolates

Published online by Cambridge University Press:  06 April 2009

P. K. Goyal  and
D. Wakelin
P. K. Goyal
Affiliation:
MRC Experimental Parasitology Group, Department of Life Science, University of Nottingham, Nottingham NG7 2RD
D. Wakelin
Affiliation:
MRC Experimental Parasitology Group, Department of Life Science, University of Nottingham, Nottingham NG7 2RD
Get access Rights & Permissions [Opens in a new window]

Summary

Antigen preparations from three isolates of Trichinella spiralis (sensu-stricto) have been tested for their ability to immunize mice against homologous and heterologous challenge infections. Immunization was measured by accelerated expulsion of adult worms, reduction in female worm fecundity, stunting of growth, and level of antibody responses before and after challenge. Antigens of two isolates, from London (L) and Poland (P), immunized effectively against homologous challenge, adult worm recoveries being reduced by 64% and 51% respectively within 6 days. Antigen from a Spanish(S) isolate gave no homologous protection in terms of worm recovery at day 6 and only 43% reduction at day 8. L- and P-isolate antigens immunized well against heterologous challenge (day 6 worm recoveries reduced by 66% and 59%) but similar protection against S-isolate challenge was not seen until day 8. Immunization with S antigen gave better protection at day 6 against L-isolate challenge (77%) than against challenge with P-isolate (37%). These results imply considerable, but not complete, antigen cross-reactivity between the isolates and this was reflected in antibody responses. These variations in immunogenicity and immunization potential are discussed in terms of constraints on the use of vaccines against parasites that are widely distributed geographically.

Keywords

vaccinationmiceimmuńe responseTrichinella spiralisisolateslarval antigensfecundity
Type
Research Article
Information
Parasitology , Volume 107 , Issue 3 , September 1993 , pp. 311 - 317
Copyright
Copyright © Cambridge University Press 1993

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

Bell, R. G., McGregor, D. D. & Despommier, D. D. (1979). Trichinella spiralis: mediation of the intestinal component of protective immunity in the rat by multiple, phase-specific antiparasite responses. Experimental Parasitology 47, 140–57.CrossRefGoogle ScholarPubMed
Bolas-Fernandez, F. & Wakelin, D. (1990). Infectivity, antigenicity and host responses to isolates of the genus Trichinella. Parasitology 100, 491–7.CrossRefGoogle ScholarPubMed
Bolas-Fernandez, F. & Wakelin, D. (1992). Immunization against geographical isolates of Trichinella spiralis in mice. International Journal for Parasitology 22, 773–81.CrossRefGoogle ScholarPubMed
Despommier, D. D., McGregor, D. D., Crum, E. D. & Carter, P. B. (1977). Immunity to Trichinella spiralis: II. Expression of immunity against adult worms. Immunology 33, 797–805.Google ScholarPubMed
Gamble, H. R. (1985). Comparison of immune effects in mice immunized with Trichinella spiralis adult and larval antigen. Journal of Parasitology 71, 680–2.CrossRefGoogle Scholar
Goyal, P. K. & Wakelin, D. (1993). Influence of variation in host strain and parasite isolate on inflammatory and antibody responses to Trichinella spiralis in mice. Parasitology 106, 371–8.CrossRefGoogle ScholarPubMed
Grencis, R. K., Crawford, C. R., Pritchard, D. I., Behnke, J. M. & Wakelin, D. (1986). Immunization of mice with surface antigens from the muscle larvae of Trichinella spiralis. Parasite Immunology 8, 587–96.CrossRefGoogle ScholarPubMed
Lowry, O. H., Rosebrough, N. J., Farr, A. C. & Randall, R. J. (1951). Protein measurement with the folin phenol reagents. Journal of Biological Chemistry 193, 265–75.CrossRefGoogle Scholar
Mitchell, G. F. (1982). New trends towards vaccination against parasites. Clinics in Immunology and Allergy 2, 721–37.CrossRefGoogle Scholar
Silberstein, D. S. (1983). Antigen. In Trichinella and Trichinosis, (ed. Campbell, W. C.), pp. 309–34. New York: Plenum Press.CrossRefGoogle Scholar
Wakelin, D. & Denham, D. A. (1983). The immune response. In Trichinella and Trichinosis, (ed. Campbell, W. C.), pp. 265–308. New York: Plenum Press.CrossRefGoogle Scholar
Wakelin, D. & Lloyd, M. (1976). Immunity to primary and challenge infection of Trichinella spiralis in mice: a re-examination of conventional parameters. Parasitology 72, 173–82.CrossRefGoogle ScholarPubMed
Wassom, D. L., Wakelin, D., Brooks, B. O., Krco, C. J. & David, C. S. (1984). Genetic control of immunity to Trichinella spiralis infection of mice. Hypothesis to explain the role of H-2 genes in primary and challenge infection. Immunology 51, 625–31.Google Scholar
Williams, J. F. (1986). Prospects for prophylaxis of parasitism. International Journal for Parasitology 17, 711–19.CrossRefGoogle Scholar