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Using MALDI-TOF MS to identify mosquitoes collected in Mali and their blood meals

Published online by Cambridge University Press:  07 February 2018

Fatalmoudou Tandina ,
Sirama Niaré ,
Maureen Laroche ,
Abdoulaye K Koné ,
Adama Z Diarra ,
Abdoulaye Ongoiba ,
Jean Michel Berenger ,
Ogobara K Doumbo ,
Didier Raoult  and
Philippe Parola
Fatalmoudou Tandina
Affiliation:
Aix Marseille Univ, IRD, AP-HM, IHU-Méditerranée Infection, SSA, VITROME, Marseille, France Department of Epidemiology of Parasitic Diseases, Malaria Research and Training Center, University of Science, Techniques and Technologies of Bamako, Mali
Sirama Niaré
Affiliation:
Aix Marseille Univ, IRD, AP-HM, IHU-Méditerranée Infection, SSA, VITROME, Marseille, France Department of Epidemiology of Parasitic Diseases, Malaria Research and Training Center, University of Science, Techniques and Technologies of Bamako, Mali
Maureen Laroche
Affiliation:
Aix Marseille Univ, IRD, AP-HM, IHU-Méditerranée Infection, SSA, VITROME, Marseille, France
Abdoulaye K Koné
Affiliation:
Department of Epidemiology of Parasitic Diseases, Malaria Research and Training Center, University of Science, Techniques and Technologies of Bamako, Mali
Adama Z Diarra
Affiliation:
Aix Marseille Univ, IRD, AP-HM, IHU-Méditerranée Infection, SSA, VITROME, Marseille, France Department of Epidemiology of Parasitic Diseases, Malaria Research and Training Center, University of Science, Techniques and Technologies of Bamako, Mali
Abdoulaye Ongoiba
Affiliation:
Department of Epidemiology of Parasitic Diseases, Malaria Research and Training Center, University of Science, Techniques and Technologies of Bamako, Mali
Jean Michel Berenger
Affiliation:
Aix Marseille Univ, IRD, AP-HM, IHU-Méditerranée Infection, SSA, VITROME, Marseille, France
Ogobara K Doumbo
Affiliation:
Department of Epidemiology of Parasitic Diseases, Malaria Research and Training Center, University of Science, Techniques and Technologies of Bamako, Mali
Didier Raoult
Affiliation:
Aix Marseille Univ, IRD, AP-HM, IHU-Méditerranée Infection, SSA, VITROME, Marseille, France
Philippe Parola*
Affiliation:
Aix Marseille Univ, IRD, AP-HM, IHU-Méditerranée Infection, SSA, VITROME, Marseille, France
*
Author for correspondence: Philippe Parola, Email: [email protected]
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Abstract

Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) has been recently described as an innovative and effective tool for identifying arthropods and mosquito blood meal sources. To test this approach in the context of an entomological survey in the field, mosquitoes were collected from five ecologically distinct areas of Mali. We successfully analysed the blood meals from 651 mosquito abdomens crushed on Whatman filter paper (WFPs) in the field using MALDI-TOF MS. The legs of 826 mosquitoes were then submitted for MALDI-TOF MS analysis in order to identify the different mosquito species. Eight mosquito species were identified, including Anopheles gambiae Giles, Anopheles coluzzii, Anopheles arabiensis, Culex quinquefasciatus, Culex neavei, Culex perexiguus, Aedes aegypti and Aedes fowleri in Mali. The field mosquitoes for which MALDI-TOF MS did not provide successful identification were not previously available in our database. These specimens were subsequently molecularly identified. The WFP blood meal sources found in this study were matched against human blood (n = 619), chicken blood (n = 9), cow blood (n = 9), donkey blood (n = 6), dog blood (n = 5) and sheep blood (n = 3). This study reinforces the fact that MALDI-TOF MS is a promising tool for entomological surveys.

Keywords

MALDI-TOF MSmosquitofieldblood mealsWhatmanMali
Type
Research Article
Information
Parasitology , Volume 145 , Issue 9 , August 2018 , pp. 1170 - 1182
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Copyright
Copyright © Cambridge University Press 2018 

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