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Ultrastructure and molecular phylogeny of Pleistophora hyphessobryconis (Microsporidia) infecting hybrid jundiara (Leiarius marmoratus × Pseudoplatystoma reticulatum) in a Brazilian aquaculture facility

Published online by Cambridge University Press:  02 November 2015

ANDREW D. WINTERS ,
INGEBORG M. LANGOHR ,
MARCOS DE A. SOUZA ,
EDSON M. COLODEL ,
MAURO P. SOARES  and
MOHAMED FAISAL
ANDREW D. WINTERS
Affiliation:
Department of Fisheries and Wildlife, Michigan State University, East Lansing, Michigan 48824, USA
INGEBORG M. LANGOHR
Affiliation:
Department of Pathobiological Sciences, Louisiana State University, Baton Rouge, Louisiana 70803, USA
MARCOS DE A. SOUZA
Affiliation:
Clinical Veterinary Medical Department, Faculty of Agronomy, Veterinary Medicine and Animal Science, Federal University of Mato Grosso, Cuiabá, Mato Grosso, Brazil
EDSON M. COLODEL
Affiliation:
Clinical Veterinary Medical Department, Faculty of Agronomy, Veterinary Medicine and Animal Science, Federal University of Mato Grosso, Cuiabá, Mato Grosso, Brazil
MAURO P. SOARES
Affiliation:
Regional Diagnostic Laboratory, Federal University of Pelotas, Pelotas, RS, Brazil
MOHAMED FAISAL*
Affiliation:
Department of Fisheries and Wildlife, Michigan State University, East Lansing, Michigan 48824, USA Pathobiology and Diagnostic Investigation, Michigan State University, East Lansing, Michigan 48824, USA
*
* Corresponding author. Department of Pathobiology and Diagnostic Investigation, College of Veterinary Medicine, Michigan State University, East Lansing, Michigan 48824, USA. E-mail: [email protected]
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Summary

A microsporidian infecting the skeletal muscle of hybrid jundiara (Leiarius marmoratus × Pseudoplatystoma reticulatum) in a commercial aquaculture facility in Brazil is described. Affected fish exhibited massive infections in the skeletal muscle that were characterized by large opaque foci throughout the affected fillets. Histologically, skeletal muscle was replaced by inflammatory cells and masses of microsporidial developmental stages. Generally pyriform spores had a wrinkled bi-layer spore wall and measured 4·0 × 6·0 µm. Multinucleate meronts surrounded by a simple plasma membrane were observed. The polar filament had an external membrane and a central electron dense mass. The development of sporoblasts within a sporophorous vesicle appeared synchronized. Ultrastructural observations and molecular analysis of 16S rDNA sequences revealed that the microsporidian was Pleistophora hyphessobryconis. This study is the first report of a P. hyphessobryconis infection in a non-ornamental fish.

Keywords

MicrosporidiaPleistophora hyphessobryconis16S rDNAaquaculture
Type
Research Article
Information
Parasitology , Volume 143 , Issue 1 , January 2016 , pp. 41 - 49
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Copyright
Copyright © Cambridge University Press 2015 

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