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Ultrastructural localization of pancreatic polypeptide- and FMRFamide immunoreactivities within the central nervous system of the nematode, Ascaris suum (Nematoda: Ascaroidea)

Published online by Cambridge University Press:  06 April 2009

D. J. A. Brownlee ,
G. P. Brennan ,
D. W. Halton ,
I. Fairweather  and
C. Shaw
D. J. A. Brownlee
Affiliation:
School of Biology and Biochemistry, The Queen's University of Belfast, Belfast BT7 INN, Northern Ireland
G. P. Brennan
Affiliation:
School of Biology and Biochemistry, The Queen's University of Belfast, Belfast BT7 INN, Northern Ireland
D. W. Halton
Affiliation:
School of Biology and Biochemistry, The Queen's University of Belfast, Belfast BT7 INN, Northern Ireland
I. Fairweather
Affiliation:
School of Biology and Biochemistry, The Queen's University of Belfast, Belfast BT7 INN, Northern Ireland
C. Shaw
Affiliation:
School of Clinical Medicine, The Queen's University of Belfast, Belfast BT7 INN, Northern Ireland
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Summary

A post-embedding immunogold technique has been used to examine the subcellular distribution of immunoreactivities to vertebrate pancreatic polypeptide (PP) and to the invertebrate peptide, FMRFamide within the central nervous system (CNS) of the nematode, Ascaris suum. Gold labelling of peptide was localized exclusively over dense-cored vesicles within nerve cell bodies, nerve axons and nerve terminals of the main ganglia and nerve cords in the CNS. Double-labelling of peptides demonstrated an apparent co-localization of PP and FMRFamide immunoreactivities in the same dense-cored vesicles, although populations of dense-cored vesicles that labelled solely for FMRFamide were also evident. Antigen preabsorption studies indicated little or no cross-reactivity between the two antisera.

Keywords

Ascaris suumNematodaneuropeptidespancreatic polypeptideFMRFamideimmunogold labellingultrastructurenervous system
Type
Research Article
Information
Parasitology , Volume 108 , Issue 5 , June 1994 , pp. 587 - 593
Copyright
Copyright © Cambridge University Press 1994

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