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Ultrastructural immunolocalization of two circulating egg antigens in miracidia of Schistosoma mansoni

Published online by Cambridge University Press:  06 April 2009

J. J. P. M. Bogers ,
H. A. M. Nibbeling ,
S. Kumar Singh ,
A. M. Deelder  and
E. A. E. Van Marck
J. J. P. M. Bogers
Affiliation:
Laboratory of Pathology, University of Antwerp (UIA), Universiteitsplein 1, B-2610 Wilrijk, Belgium
H. A. M. Nibbeling
Affiliation:
Laboratory of Parasitology, University of Leiden, Leiden, The, Netherlands
S. Kumar Singh
Affiliation:
Laboratory of Pathology, University of Antwerp (UIA), Universiteitsplein 1, B-2610 Wilrijk, Belgium
A. M. Deelder
Affiliation:
Laboratory of Parasitology, University of Leiden, Leiden, The, Netherlands
E. A. E. Van Marck
Affiliation:
Laboratory of Pathology, University of Antwerp (UIA), Universiteitsplein 1, B-2610 Wilrijk, Belgium
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Summary

In the present study ultrastructural immunocytochemical techniques have been used to localize the cells producing two soluble egg antigens present in the hatching fluid of Schistosoma mansoni, using two recently described monoclonal antibodies (MAbs). In the miracidium of S. mansoni, the one apical and the two lateral unicellular glands showed immunoreactivity for the egg antigens, using a post-embedding immunogold technique. Immunoreactivity was associated with the contents of vesicles for both the MAbs, although the intravesicular distribution was not identical. This difference in ultrastructural staining pattern provides additional evidence that the two MAbs are binding with different epitopes of the same antigen or with different antigens altogether. The finding of free unbound label around the embedded miracidium is consistent with secretion having been released from the glands.

Keywords

Schistosoma mansonimiracidiumsoluble egg antigenelectron microscopyimmunogold labelling
Type
Research Article
Information
Parasitology , Volume 110 , Issue 4 , May 1995 , pp. 365 - 370
Copyright
Copyright © Cambridge University Press 1995

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