Hostname: page-component-586b7cd67f-vdxz6 Total loading time: 0 Render date: 2024-12-01T00:24:57.284Z Has data issue: false hasContentIssue false
  • English
  • Français

Ultrastructural and cytochemical observations on the body wall of the redia of Sphaeridiotrema globulus (Rudolphi, 1819)

Published online by Cambridge University Press:  06 April 2009

Trevor A. J. Reader
Trevor A. J. Reader
Affiliation:
Department of Biological Sciences, Portsmouth Polytechnic, Park Road, Portsmouth, Hants.
Get access Rights & Permissions [Opens in a new window]

Extract

The ultrastructure of the body wall of the redia of Sphaeridiotrema globulus is described. The tegument, which possesses numerous microvilli, is shown to be a syncytial, cytoplasmic layer connected to underlying subtegumentary ‘cells’ by cytoplasmic processes. Between the subtegumentary ‘cells’ are located the normal parenchyma cells of the body wall, which are believed to be important in carbohydrate storage.Mitochondria, Golgi complexes, endoplasmic reticulum and beta glycogen granules are located in the tegument and subtegumentary ‘cells’. In addition, large whorled bodies and dense secretory granules appear to be formed within the subtegumentary ‘cells’ prior to their passage into the outer tegument. It is suggested that these whorled structures are contributing to the growth of the tegument. Small membrane-bound ‘vesicles’ are also seen in the tegument and some of these may be pinocytotic in nature.Following incubation, horseradish peroxidase tracer was localized within ‘vesicles’ in both the tegument, subtegumentary ‘cells’ and parenchyma cells, which indicates that the redial body wall may be important in the uptake of nutrients.Phosphatase enzymes are abundant within the tegument, particularly in association with the plasma membranes and microvilli. These enzymes, which appear to have their origin in the endoplasmic reticulum of subtegumentary cells, are believed to be associated with the uptake of nutrients through the redial tegument.

Type
Research Article
Information
Parasitology , Volume 65 , Issue 3 , December 1972 , pp. 537 - 546
Copyright
Copyright © Cambridge University Press 1972

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Belton, C. M. & Harms, A. J. (1967). Fine structure of the cuticle of the cercaria of Acanthatrium oregonense (Macy). The Journal of Parasitology 53, 715–24.CrossRefGoogle ScholarPubMed
Bils, R. F. & Martin, W. E. (1966). Fine structure and development of the trematode integument. Transactions of the American Microscopical Society 85, 78–88.CrossRefGoogle ScholarPubMed
Bjorkman, N. & Thorsell, W. (1964). On the fine structure and resorptive function of the cuticle of the liver fluke Fasciola hepatica L. Experimental Cell Research 33, 319–29.CrossRefGoogle Scholar
Burton, P. R. (1964). The ultrastructure of the integument of the frog lung-fluke, Haematoloechus medioplexus (Trematoda: Plagiorchiidae). Journal of Morphology 115, 305–18.CrossRefGoogle ScholarPubMed
Burton, P. R. (1966). The ultrastructure of the integument of the frog bladder-fluke Gorgoderina sp. The Journal of Parasitology 52, 926–34.CrossRefGoogle ScholarPubMed
Charles, G. H. (1970). The ultrastructure of the developing pseudophyllid tegument (epidermis) with reference to the larval stages of Schistocephalus solidus and Ligula intestinalis. The Journal of Parasitology 56. 2nd International Congress of Parasitology, Washington, D.C. Proceedings, Part 4, pp. 38–9.Google Scholar
Cheah, K. S. (1967). Histochemical and spectrophotometric demonstration of peroxidase in Monieza expansa (Cestoda). Comparative Biochemistry and Physiology 21, 351–5.CrossRefGoogle Scholar
Clegg, J. A. (1972). The schistosome surface in relation to parasitism. In Functional aspects of Parasite Surfaces, ed. Taylor, A. E. R., and Muller, R.. London: Blackwell Scientific Publications.Google Scholar
Dixon, K. E. (1970). Absorption by developing cercariae of Cloacitrema narrabeenensis (Philophthalmidae). Journal of Parasitology 56. 2nd International Congress of Parasitology, Washington, D.C. Proceedings, Part 2, pp. 416–17.Google Scholar
Erasmus, D. A. (1968). The host-parasite interface of Cyaihocotyle bushiensis, Khan, 1962 (Trematoda: Strigeoidea). III. Electron microscope observations on non-specific phosphatase activity. Parasitology 58 371–5.CrossRefGoogle Scholar
Erasmus, D. A. & Ohman, C. (1963). The structure and function of the adhesive organ in strigeid trematodes. Annals of the New York Academy of Sciences 113 7–35.CrossRefGoogle ScholarPubMed
Goldfischer, S., Carasso, N. & Favard, P. (1963). The demonstration of acid phosphatase activity by electron microscopy in the ergastoplasm of the ciliate Campanella umbellaria L. Journal de Microscopie 2 621–8.Google Scholar
Graham, R. C. & Karnovsky, M. J. (1966). The early stages of absorption of injected horseradish peroxidase in the proximal tubules of mouse kidney. Ultrastructural cytochemistry by a new technique. Journal of Histochemistry and Cytochemistry 14, 291.CrossRefGoogle ScholarPubMed
Halton, D. W. (1968). Peroxidase activity in the trematode Haplometra cylindricea. Experimental Parasitology 24 265–9.CrossRefGoogle Scholar
Hugon, J. & Borgers, M. (1966). Ultrastructural localisation of alkaline phosphatase activity in the absorbing cells of the duodenum of mouse. Journal of Histochemistry and Cytochemistry 14 629–40.CrossRefGoogle ScholarPubMed
Jenkinson, J. A. (1968). Comparative ultrastructural and histochemical studies of larval trematodes with special reference to gland cells and the integument. Ph.D. Thesis, University of Wales.Google Scholar
Køie, M. (1971 a). On the histochemistry and ultrastructure of the redia of Neophasis lageniformis (Lebour, 1910) (Trematoda: Acanthocolpidae). Ophelia 9, 113–43.CrossRefGoogle Scholar
Køie, M.. (1971 b). On the histochemistry and ultrastructure of the daughter sporocyst of Cercaria buccini Lebour, 1911. Ophelia 9, 145–63.CrossRefGoogle Scholar
Krupa, P. L., Bal, A. K. & Cousineau, G. H. (1967). Ultrastructure of the redia of Cryptocotyle lingua. Journal of Parasitology 53, 725–34.CrossRefGoogle ScholarPubMed
Krupa, P. L., Cousineau, G. H. & Bal, A. K. (1968). Ultrastructural and histochemical observations on the body wall of Cryptocotyle lingua rediae (Trematoda). Journal of Parasitology 54, 900–8.CrossRefGoogle Scholar
Lumsden, R. D., Gonzales, G., Mills, R. R. & Viles, J. M. (1968). Cytological studies on the absorptive surfaces of cestodes. III. Hydrolysis of phosphate esters. Journal of Parasitology 54, 524–35.CrossRefGoogle Scholar
Matricon-Gondran, M. (1969). Étude ultrastructurale du syncytium tégumentaire et de son évolution chez des trématodes digénétiques larvaires. Compte rendu hebdomadaire des Siances de L'Academie des Sciences 269, 2384–7.Google Scholar
Rees, G. (1966). Light and electron microscope studies of the redia of Parorchis acanthus. Parasitology 56 589–602.CrossRefGoogle ScholarPubMed
Rees, G. (1971). The ultrastruoture of the epidermis of the redia and cercaria of Parorchis acanthus Nicoll. A study by scanning and transmission electron microscopy. Parasitology 62 479–88.CrossRefGoogle Scholar
Smith, J. H., Reynolds, E. S. & von Lichtenberg, F. (1969). The integument of Schistosoma mansoni. The American Journal of Tropical Medicine and Hygiene 18 28–49.CrossRefGoogle ScholarPubMed
Szidat, L. (1937). Über die Entwicklungsgeschichte von Sphaeridiotrema globulus Rud. 1814 und die Stellung der Psilostomidae Odhner im naturlichen System. 1. Die Entwicklungsgeschichte von Sphaeridiotrema globulus Rud. Zeitschrift für Parasitenhunde 529–42.CrossRefGoogle Scholar
Threadgold, L. T. (1963). The tegument and associated structures of Fasciola hepatica. Quarterly Journal of Microscopical Science 104 505–12.Google Scholar
Threadgold, L. T. (1968). Electron microscope studies of Fasciola hepatica. VI. The ultrastructural localisation of phosphatases. Experimental Parasitology 23, 264–76.CrossRefGoogle ScholarPubMed
Threadgold, L. T., Arme, C. & Read, C. P. (1968). The ultrastructural localisation of a peroxidase in the tapeworm Hymenolepis diminuta. Journal of Parasitology 54 802–7.CrossRefGoogle ScholarPubMed
Threadgold, L. T. & Read, C. P. (1968). Electron microscopy of Fasciola hepatica. V. Peroxidase localisation. Experimental Parasitology 23 221–7.CrossRefGoogle Scholar