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Tissue expression of the Schistosoma mansoni 28 kDa glutathione S-transferase

Published online by Cambridge University Press:  06 April 2009

E. Porchet ,
A. McNair ,
A. Caron ,
J. P. Kusnierz ,
K. Zemzoumi  and
A. Capron
E. Porchet
Affiliation:
Centre d'Immunologie et de Biologie Parasitaire, INSERM U 167, Institut Pasteur, 1 Rue du Professeur Calmette, B.P. 245, F-59019 Lille Cédex
A. McNair
Affiliation:
Centre d'Immunologie et de Biologie Parasitaire, INSERM U 167, Institut Pasteur, 1 Rue du Professeur Calmette, B.P. 245, F-59019 Lille Cédex
A. Caron
Affiliation:
Centre d'Immunologie et de Biologie Parasitaire, INSERM U 167, Institut Pasteur, 1 Rue du Professeur Calmette, B.P. 245, F-59019 Lille Cédex
J. P. Kusnierz
Affiliation:
Centre d'Immunologie et de Biologie Parasitaire, INSERM U 167, Institut Pasteur, 1 Rue du Professeur Calmette, B.P. 245, F-59019 Lille Cédex
K. Zemzoumi
Affiliation:
Centre d'Immunologie et de Biologie Parasitaire, INSERM U 167, Institut Pasteur, 1 Rue du Professeur Calmette, B.P. 245, F-59019 Lille Cédex
A. Capron
Affiliation:
Centre d'Immunologie et de Biologie Parasitaire, INSERM U 167, Institut Pasteur, 1 Rue du Professeur Calmette, B.P. 245, F-59019 Lille Cédex
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Extract

The expression of the Schistosoma mansoni 28 kDa glutathione S-transferase (Sm28) was studied using molecular (PCR, in situ hybridization), and immunocytochemical techniques. The presence of Sm28 was demonstrated in all developmental stages of the parasite except the intra-uterine immature egg. In the parenchyma of male and female adult worms the distribution of Sm28 was limited to a subpopulation of parenchymal cells and to the dorsal tubercles of the male. The tegument, the muscles, the digestive tract, the neural mass, the vitelline glands, and mature gametes were not immunoreactive. Immature germinal cells in both sexes, and the ootype in the female genital system, were found to express Sm28. Deposits of immunoreactive material on host skin following cercarial penetration, exfoliation from the male tubercles, and especially emission of Sm28 from eggs in hepatic granulomas are suspected to be a source of antigen during the parasite infection. The reduction in worm fecundity previously observed in immunization experiments may result from an antibody response directed against Sm28 present in the ootype. There was no cross-reactivity observed, under the experimental conditions used, between the anti-Sm28 sera and either vertebrate or invertebrate host tissue.

Keywords

Schistosoma mansoniglutathione S-transferasedevelopmental stages
Type
Research Article
Information
Parasitology , Volume 109 , Issue 5 , December 1994 , pp. 565 - 572
Copyright
Copyright © Cambridge University Press 1994

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References

REFERENCES

Balloul, J. M., Gryzch, J. M., Pierce, R. J. & Capron, A. (1987 a). A purified 28,000 dalton protein from Schistosoma mansoni adult worms protects rats and mice against experimental schistosomiasis. Journal of Immunology 138, 3448–53.CrossRefGoogle Scholar
Balloul, J. M., Sondermeyer, P., Dreyer, D., Capron, M., Grzych, J. M., Pierce, R. J., Carvallo, D., Lecocq, J. P. & Capron, A. (1987 b). Molecular cloning of a protective antigen against schistosomiasis. Nature, London 326, 194–53.CrossRefGoogle Scholar
Basch, P. F. (1991). Schistosomes. New York, Oxford: Oxford University Press.Google Scholar
Boulanger, D., Reid, G. D. F., Sturrock, R. F., Wolowczuck, I., Balloul, J. M., Grezel, D., Pierce, R. J., Otieno, M. F., Guerret, S., Grimaud, J. A., Butterworth, A. E. & Capron, A. (1991). Immunization of mice and baboons with the recombinant Sm28GST affects both worm viability and fecundity after experimental infections with Schistosoma mansoni. Parasite Immunology 13, 473–90.CrossRefGoogle ScholarPubMed
Capron, A. (1992). Vaccine strategies against schistosomiasis. Immunobiology 184, 282–94.CrossRefGoogle ScholarPubMed
Capron, A., Dessaint, J. P., Capron, M., Ouma, J. H. & Butterworth, A. E. (1987). Immunity to schistosomes. Progress toward vaccine. Science 238, 1065–72.CrossRefGoogle ScholarPubMed
Cordingley, J. S. (1987). Trematode eggshells: novel protein biopolymers. Parasitology Today 3, 341–4.CrossRefGoogle ScholarPubMed
Grzych, J. M., Grezel, D., Xu, C. B., Neyrinck, J. L., Capron, M., Ouma, J. H., Butterworth, E. & Capron, A. (1993). IgA antibodies to a protective antigen in human Schistosomiasis mansoni. Journal of Immunology 150, 527–35.CrossRefGoogle ScholarPubMed
Hockley, D. J. (1973). Ultrastructure of the tegument of Schistosoma. Advances in Parasitology 11, 233–305.CrossRefGoogle ScholarPubMed
Holy, J. M., O'Leary, K. A., Oaks, J. A. & Tracy, J. W. (1989). Immunocytochemical localization of the major GST in adult S. mansoni. Journal of Parasitology 75, 181–90.CrossRefGoogle Scholar
Imbert-Establet, D. & Combes, C. (1992). Relocation of Schistosoma mansoni in the lungs and resistance to reinfection in Rattus rattus. Parasitology 104, 51–7.CrossRefGoogle ScholarPubMed
Kaplan, B. B., Bernstein, S. L. & Gioio, A. E. (1979). An improved method for the rapid isolation of brain ribonucleic acid. The Biochemical Journal 183, 181–4.CrossRefGoogle ScholarPubMed
Linder, E. (1990). Cercarial kissing marks. No superficial make up. Parasitology Today 6, 393–5.Google Scholar
Mehlhorn, H. (1988). Parasitology in Focus. Berlin: Springer-Verlag.CrossRefGoogle Scholar
Mitchell, G. F. (1989). Glutathione S-transferases. Potential components of anti-schistosome vaccines? Parasitology Today 5, 34–7.Google Scholar
Smith, D. B., Davern, K. M., Board, P. M., Tiu, W. V., Garcia, E. G. & Mitchell, G. F. (1986). M r 26,000 antigen of Schistosoma japonicum recognized by resistant WEHI 129/J mice is a parasite glutathione- S-transferase. Proceedings of the National Academy of Sciences, USA 83, 8703.Google Scholar
Smith, J. H., Reynolds, E. S. & Von Lichtenberg, F. (1969). The integument of Schistosoma mansoni. American Journal of Tropical Medicine and Hygiene 18, 28–49.Google Scholar
Taylor, J. B., Vidal, A., Torpier, G., Meyer, D. J., Roitsch, C., Balloul, J. M., Southan, C., Sondermeyer, P., Pemble, S., Lecocq, J. P., Capron, A. & Ketterer, B. (1988). The glutathione transferase activity and tissue distribution of a cloned M r protective antigen of Schistosoma mansoni. EMBO Journal 7, 465–72.CrossRefGoogle ScholarPubMed
Trottein, F., Kieny, M. P., Verwaerde, C., Torpier, G., Pierce, R. J., Balloul, J. M., Schmitt, D., Lecocq, J. P. & Capron, C. (1990). Molecular cloning and tissue distribution of a 26 kDa Schistosoma mansoni glutathione S-transferase. Molecular and Biochemical Parasitology 41, 35–44.CrossRefGoogle ScholarPubMed
Van Dam, G. J., Kornelis, D., Van Zeyl, R. J. M., Rotmans, J. P. & Deelder, A. (1993). Schistosoma mansoni: analysis of monoclonal antibodies reactive with gut-associated antigens. Parasitology Research 79, 55–62.Google Scholar
Walker, J., Crowley, P., Moreman, A. D. & Barrett, J. (1993). Biochemical properties of cloned glutathione S-transferases from Schistosoma mansoni and Schistosoma japonicum. Molecular and Biochemical Parasitology 61, 255–64.Google Scholar
Wijffels, G. L., Sexton, J. L., Salvatore, L., Pettitt, J. M., Humphris, D. C., Panaccio, M. & Spithill, T. W. (1992). Primary sequence heterogeneity and tissue expression of glutathione S-transferases of Fasciola hepatica. Experimental Parasitology 74, 87–9.CrossRefGoogle ScholarPubMed
Xu, C. B., Verwaerde, C., Gryzch, J. M., Fontaine, J. & Capron, A. (1991). A monoclonal antibody blocking the Schistosoma mansoni 28 kDa glutathione S-transferase activity reduces female worm fecundity and egg viability. European Journal of Immunology 21, 1801–7.CrossRefGoogle ScholarPubMed