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Susceptibility to hydrogen peroxide of Plasmodium falciparum infecting glucose-6-phosphate dehydrogenase-deficient erythrocytes

Published online by Cambridge University Press:  06 April 2009

S. Kamchonwongpaisan ,
A. Bunyaratvej ,
W. Wanachiwanawin  and
Y. Yuthavong
S. Kamchonwongpaisan
Affiliation:
1Department of Pathology, Faculty of Medicine, Ramathibodi Hospital
A. Bunyaratvej
Affiliation:
1Department of Pathology, Faculty of Medicine, Ramathibodi Hospital
W. Wanachiwanawin
Affiliation:
2Department of Medicine, Faculty of Medicine, Siriraj Hospital
Y. Yuthavong
Affiliation:
3Department of Biochemistry, Faculty of Science, Mahidol University, Bangkok, Thailand
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Summary

The susceptibility to oxidant-mediated killing of Plasmodium falciparum infecting normal and glucose-6-phosphate dehydrogenase (G6PD)-deficient erythrocytes was assessed by exposure to hydrogen peroxide generated by the glucose–glucose oxidase system. The parasites infecting G6PD-deficient erythrocytes had markedly greater susceptibility to hydrogen peroxide under a variety of conditions than those infecting normal erythrocytes. In both cases, the killing effect was mediated mainly through the host cells since treatment of the erythrocytes with hydrogen peroxide did not change their relative susceptibility. The parasites were most susceptible during maturation, especially in G6PD-deficient erythrocytes, although a reduction in parasite invasion was also observed. The role of oxidant-mediated killing in the protection of G6PD-deficient hosts from P. falciparum infection is discussed.

Keywords

Plasmodium falciparumhydrogen peroxideglucose-6-phosphate dehydrogenasesusceptibility
Type
Research Article
Information
Parasitology , Volume 99 , Issue 2 , October 1989 , pp. 171 - 174
Copyright
Copyright © Cambridge University Press 1989

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