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Surface antigens of Toxoplasma gondii

Published online by Cambridge University Press:  06 April 2009

G. Couvreur ,
A. Sadak ,
B. Fortier  and
J. F. Dubremetz
G. Couvreur
Affiliation:
U42 INSERM, 369 rue J. Guesde, 59650 Villeneuve d'Ascq, France
A. Sadak
Affiliation:
U42 INSERM, 369 rue J. Guesde, 59650 Villeneuve d'Ascq, France
B. Fortier
Affiliation:
U42 INSERM, 369 rue J. Guesde, 59650 Villeneuve d'Ascq, France
J. F. Dubremetz*
Affiliation:
U42 INSERM, 369 rue J. Guesde, 59650 Villeneuve d'Ascq, France
*
Reprint requests to Dr J. F. Dubremetz.
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Summary

Monoclonal antibodies reacting with pellicular antigens of Toxoplasma gondii tachyzoites have been selected among hybridomas produced against this organism by immunofluorescence assay. These antigens have been further characterized by immunofluorescence on living zoites, Western immunoblotting and immunoprecipitation of lactoperoxidase surface radio-iodinated tachyzoite lysates. The simultaneous characterization of 5 different surface antigens (P43, P35, P30, P23, P22) some of which have already been studied individually allowed a better definition of these antigens and the characterization of a yet undescribed surface molecule (P23).

Type
Research Article
Information
Parasitology , Volume 97 , Issue 1 , August 1988 , pp. 1 - 10
Copyright
Copyright © Cambridge University Press 1988

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References

REFERENCES

Dubremetz, J.-F., Rodriguez, C. & Ferreira, E. (1985). Toxoplasma gondii: redistribution of monoclonal antibodies on tachyzoites during host cell invasion. Experimental Parasitology 59, 24–32.CrossRefGoogle ScholarPubMed
Galfre, G., Howe, S. C., Milstein, C., Butcher, G. W. & Howard, J. C. (1977). Antibodies to major histocompatibility antigens produced by hybrid cell lines. Nature, London 266, 550–2.CrossRefGoogle ScholarPubMed
Grimwood, B. G., Hechemy, K. & Stevens, R. W. (1979). Toxoplasma gondii: Purification of trophozoites propagated in cell culture. Experimental Parasitology 48, 282–6.CrossRefGoogle ScholarPubMed
Handman, E., Goding, J. W. & Remington, J. S. (1980). Detection and characterization of membrane antigens of Toxoplasma gondii. Journal of Immunology 124, 2578–83.CrossRefGoogle ScholarPubMed
Johnson, A. M., McDonald, P. J. & Neoh, S. H. (1983). Monoclonal antibodies to Toxoplasma cell membrane surface antigens protect mice from Toxoplasmosis. Journal of Protozoology 30, 351–6.CrossRefGoogle ScholarPubMed
Kasper, L. H. (1987). Isolation and characterization of a monoclonal anti-P30 antibody resistant mutant of Toxoplasma gondii. Parasite Immunology 9, 433–45.CrossRefGoogle ScholarPubMed
Kasper, L. H., Bradley, M. S. & Pfefferkorn, E. R. (1984). Identification of stage-specific sporozoite antigens of Toxoplasma gondii by monoclonal antibodies. Journal of Immunology 132, 443–9.CrossRefGoogle ScholarPubMed
Kasper, L. H., Crabb, J. H. & Pfefferkorn, E. R. (1982). Isolation and characterization of monoclonal antibody-resistant antigenic mutant of Toxoplasma gondii. Journal of Immunology 129, 1696–9.Google ScholarPubMed
Kasper, L. H., Crabb, J. H. & Pfefferkorn, E. R. (1983). Purification of a major membrane protein of Toxoplasma gondii by immunoabsorption with a monoclonal antibody. Journal of Immunology 130, 2407–12.CrossRefGoogle Scholar
Kasper, H. L., Currie, K. M. & Bradley, M. S. (1985). An unexpected response to vaccination with a purified major membrane tachyzoite antigen (P30) of Toxoplasma gondii. Journal of Immunology 134, 3436–51.Google ScholarPubMed
Laemmli, U. K. (1970). Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, London 227, 680–5.CrossRefGoogle ScholarPubMed
Laugier, M. & Quilici, M. (1970). Interet experimental d'une souche de Toxoplasme peu pathogène pour la souris. Annales de Parasitologie Humaine et Comparée 45, 389–403.CrossRefGoogle Scholar
Marchalonis, J. J., Cone, R. E. & Santer, V. (1971). Enzyme iodination: a probe for accessible surface proteins of normal and neoplastic lymphocytes. Journal of Biochemistry 124, 921–7.CrossRefGoogle ScholarPubMed
Rodriguez, C., Afchain, D., Capron, A., Dissous, C. & Santoro, F. (1985). Major surface protein of Toxoplasma gondii (P30) contains an immunodominant region with repetitive epitopes. European Journal of Immunology 15, 747–9.CrossRefGoogle Scholar
Sabin, A. B. (1941). Toxoplasmic encephalitis in children. Journal of the American Medical Association 116, 801–7.CrossRefGoogle Scholar
Towbin, H., Stahelin, T. & Gordon, J. (1978). Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proceedings of the National Academy of Sciences, USA 76, 4350–4.CrossRefGoogle Scholar