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A study of Trypanosoma cruzi with the electron microscope

Published online by Cambridge University Press:  06 April 2009

Hertha Meyer  and
Keith R. Porter
Hertha Meyer
Affiliation:
The Rockefeller Institute for Medical Research, New York, New York
Keith R. Porter
Affiliation:
The Rockefeller Institute for Medical Research, New York, New York
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Extract

Trypanosoma cruzi has been examined with the electron microscope. Crithidia and metacyclic forms of a blood agar culture have been studied and compared with the adult tissue forms, obtained in tissue cultures, from the same strain.

In all the forms studied, the internal structure of the body is not revealed because it is too thick for electron penetration. In places where a good penetration has been obtained the cytoplasm shows a fine striation in spiral or parallel arrangement. This striation can be digested with trypsin or by prolonged osmic acid fixation.

Dense spherical bodies of variable size can also be observed in the body portion. They can be dissolved with xylene.

The whole body of the trypanosome is covered by a fine, transparent sheath.

The flagellum is formed by the axoneme which consists of five or nine fibres. It can be followed throughout the whole length of the metacyclic and of the adult tissue forms. In the crithidia forms of the blood-agar culture, it disappears in the anterior part of the body. The whole flagellum is covered by a fine sheath. The axial fibre bundle can be digested with trypsin or by prolonged osmic-acid fixation. Both, the striation and the fibre bundle are contractile structures which could explain the movements of the trypanosome.

Some dividing forms are also described.

Type
Research Article
Information
Parasitology , Volume 44 , Issue 1-2 , May 1954 , pp. 16 - 23
Copyright
Copyright © Cambridge University Press 1954

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References

REFERENCES

Brown, H. P. (1945). Ohio J. Sci. 45, 247–301.Google Scholar
Doflein, F. (1929). Lehrbuch der Protozoenkunde. 5 Aufl. Jena: Gustav Fischer.Google Scholar
Emmel, L., Goelz, H. & Jacob, A. (1942). Dtsch. tropenmed. Z. A, 46, 573–5.Google Scholar
Emmel, L., Jacob, A. & Goelz, H. (1942). Dtsch. tropenmed. Z. A, 46, 344–8.Google Scholar
Fawcett, D. W. & Porter, K. R. (1952). Anat. Rec. 113, 539.Google Scholar
Grigg, G. W. & Hodge, A. J. (1949). Aust. J. Sci. Res. B, 2, 271–86.Google Scholar
Kraneveld, F. C., Houwink, A. L. & Keidel, H. J. W. (1951). Proc. Acad. Sci. Amst. C 54, 392–9.Google Scholar
Lofgren, R. (1950). J. Bact. 60, 617.CrossRefGoogle Scholar
Manton, I. & Clarke, B. (1951). J. Exp. Bot. 2, 125–8.CrossRefGoogle Scholar
Meyer, H. & Xavier De Olivera, M. (1948). Parasitology, 39, 91–4.CrossRefGoogle Scholar
Porter, K. R. (1953). J. Exp. Med. 97 (in the Press).CrossRefGoogle Scholar
Porter, K. R., Claude, A. & Fullam, E. (1945). J. Exp. Med. 81, 233–46.CrossRefGoogle Scholar
Saxe, L. H. (1947). Anat. Rec. 99, 687.Google Scholar
Sedar, A. W., Beams, H. W. & Janney, C. D. (1952). Proc. Soc. Exp. Biol., N.Y., 79, 303.CrossRefGoogle Scholar
Wenyon, C. M. (1926). Protozoology: a Manual for Medical Men, Veterinarians and Zoologists. New York: Wm. Wood and Comp.CrossRefGoogle Scholar