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Strongyloides ratti: transplantation of adults recovered from the small intestine at different days after infection into the colon of naive and infection-primed Wistar rats, and the effect of antioxidant treatment on large intestinal parasitism

Published online by Cambridge University Press:  16 June 2011

Y. SHINTOKU ,
H. TAKAGI ,
T. KADOSAKA ,
F. NAGAOKA ,
S. KONDO ,
M. ITOH ,
S. HONDA  and
E. KIMURA
Y. SHINTOKU
Affiliation:
Department of Parasitology, Aichi Medical University, 21 Karimata, Yazako, Nagakute, Aichi-ken 480-1195, Japan
H. TAKAGI
Affiliation:
Department of Parasitology, Aichi Medical University, 21 Karimata, Yazako, Nagakute, Aichi-ken 480-1195, Japan
T. KADOSAKA
Affiliation:
Department of Parasitology, Aichi Medical University, 21 Karimata, Yazako, Nagakute, Aichi-ken 480-1195, Japan
F. NAGAOKA
Affiliation:
Department of Parasitology, Aichi Medical University, 21 Karimata, Yazako, Nagakute, Aichi-ken 480-1195, Japan
S. KONDO
Affiliation:
Department of Parasitology, Aichi Medical University, 21 Karimata, Yazako, Nagakute, Aichi-ken 480-1195, Japan
M. ITOH
Affiliation:
Department of Parasitology, Aichi Medical University, 21 Karimata, Yazako, Nagakute, Aichi-ken 480-1195, Japan
S. HONDA
Affiliation:
Department of Parasitology, Aichi Medical University, 21 Karimata, Yazako, Nagakute, Aichi-ken 480-1195, Japan
E. KIMURA*
Affiliation:
Department of Parasitology, Aichi Medical University, 21 Karimata, Yazako, Nagakute, Aichi-ken 480-1195, Japan
*
*Corresponding author: Tel: +81 561 62 3311. Fax: +81 561 63 3645. E-mail: [email protected]
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Summary

Strongyloides ratti (Nagoya strain) is unique in that a portion of adults parasitizing the small intestine withstands ‘worm expulsion’, which starts at around day 8 post-infection (p.i.) by host immunity, and establishes in the large intestine after day 19 p.i. To investigate the mechanism, adults obtained from the small intestine at day 7 or 19 p.i. were transplanted into the colon of infection-primed immune rats. Adults obtained at day 7 p.i. were rejected quickly, whereas those obtained at day 19 p.i. could establish infection. Moreover, the body length and the number of intrauterine eggs increased in the large intestine. In a separate experiment, large intestinal parasitism was abolished by the treatment of host rats with an anti-oxidant, butylated hydroxyanisole. These results indicate that small intestinal adults between days 7 and 19 p.i. acquired the ability to parasitize the large intestine of immune rats, and that free radicals produced by the host may have played a significant role in the process.

Keywords

large intestinetransplantationBHAsizefecundity
Type
Research Article
Information
Parasitology , Volume 138 , Issue 8 , July 2011 , pp. 1053 - 1060
Copyright
Copyright © Cambridge University Press 2011

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