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Stimulation of cell division and DNA replication in Prototheca richardsi by passage through larval amphibian guts

Published online by Cambridge University Press:  06 April 2009

T. J. C. Beebee  and
A. L.-C. Wong
T. J. C. Beebee
Affiliation:
Department of Biochemistry, University of Sussex, Falmer, Brighton BN1 9QG, UK
A. L.-C. Wong
Affiliation:
Department of Biochemistry, University of Sussex, Falmer, Brighton BN1 9QG, UK
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Summary

Prototheca richardsi, an unpigmented heterotrophic alga, causes growth inhibition in amphibian larvae and has proved refractory to culture in Vitro. P. richardsi replication is dependent on regular passaging through tadpole digestive systems; uptake of thymidine by free-living Prototheca cells and incorporation into DNA are very low by comparison with leucine uptake and incorporation into protein, but DNA synthesis is detectable in cells isolated from tadpole intestines. DNA replication was elicited 6–8 h after ingestion in protothecans fed to tadpoles and subsequently re-isolated from them, providing that the tadpoles were fed subsequent to the ingestion. It appears that passaging through tadpole intestines provides an essential stimulus to maintaining an active cell division cycle in P. richardsi.

Keywords

Prototheca richardsicell divisionDNA replication
Type
Research Article
Information
Parasitology , Volume 107 , Issue 2 , August 1993 , pp. 119 - 124
Copyright
Copyright © Cambridge University Press 1993

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References

REFERENCES

Banks, B. & Beebee, T. J. C. (1987). Spawn predation and larval growth inhibition as mechanisms for niche separation in anurans. Oecologia 72, 569–73.CrossRefGoogle ScholarPubMed
Beebee, T. J. C. (1991). Purification of an agent causing growth inhibition in anuran larvae and its identification as a unicellular unpigmented alga. Canadian Journal of Zoology 69, 2146–53.CrossRefGoogle Scholar
Beebee, T. J. C. & Wong, A. L.-C. (1992 a). Prototheca-mediated interference competition between anuran larvae operates by resource diversion. Physiological Zoology 65, 805–13.CrossRefGoogle Scholar
Beebee, T. J. C. & Wong, A. L.-C. (1992 b). Leucine uptake by enterobacterial and algal members of larval anuran gut flora. Comparative Biochemistry and Physiology 101B, 527–30.Google Scholar
Dickman, C. R. (1992). Commensal and mutualistic interactions among terrestrial vertebrates. Trends in Ecology and Evolution 7, 194–7.CrossRefGoogle ScholarPubMed
Griffiths, R. A. (1991). Competition between common frog, Rana temporaria, and natterjack toad, Bufo calamita tadpoles: the effect of competitor density and interaction level on tadpole development. Oikos 61, 187–96.CrossRefGoogle Scholar
Huss, V. A. R. & Sogin, M. L. (1990). Phylogenetic position of some Chlorella species within the Chlorococcales based upon complete small-subunit ribosomal RNA sequences. Journal of Molecular Evolution 31, 432–42.CrossRefGoogle ScholarPubMed
Maniatis, T., Fritsch, E. F. & Sambrook, J. (1982). Molecular Cloning: a Laboratory Manual. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory.Google Scholar
Mann, W. & Jeffery, J. (1989). Isolation of DNA from yeasts. Analytical Biochemistry 178, 82–7.CrossRefGoogle ScholarPubMed
Pore, R. S. (1973). Selective medium for the isolation of Prototheca. Applied Microbiology 26, 648–9.CrossRefGoogle ScholarPubMed
Pore, R. S. (1985). Prototheca taxonomy. Mycopathologia 90, 129–39.CrossRefGoogle Scholar
Pore, R. S. (1986). The association of Prototheca species with slime flux in Ulmus americana and other trees. Mycopathologia 94, 67–73.CrossRefGoogle Scholar
Pore, R. S., Barnett, E. A., Barnes, W. C. & Walker, J. D. (1983). Prototheca ecology. Mycopathologia 81, 49–62.CrossRefGoogle ScholarPubMed