Hostname: page-component-586b7cd67f-rcrh6 Total loading time: 0 Render date: 2024-11-26T10:10:20.620Z Has data issue: false hasContentIssue false
  • English
  • Français

Specific systemic IgG1, IgG2 and IgM responses in pigs immunized with infective eggs or selected antigens of Ascaris suum

Published online by Cambridge University Press:  09 October 2003

E. FRONTERA ,
A. CARRÓN ,
F. J. SERRANO ,
A. ROEPSTORFF ,
D. REINA  and
I. NAVARRETE
E. FRONTERA
Affiliation:
Parasitology Section, Department of Medicine and Animal Health, Facultad de Veterinaria, Universidad de Extremadura, Cáceres, Spain
A. CARRÓN
Affiliation:
Parasitology Section, Department of Medicine and Animal Health, Facultad de Veterinaria, Universidad de Extremadura, Cáceres, Spain
F. J. SERRANO
Affiliation:
Parasitology Section, Department of Medicine and Animal Health, Facultad de Veterinaria, Universidad de Extremadura, Cáceres, Spain
A. ROEPSTORFF
Affiliation:
Danish Center for Experimental Parasitology, The Royal Veterinary and Agricultural University, Dyerlaegevej 100, DK-1870 Frederiksberg C, Copenhagen, Denmark
D. REINA
Affiliation:
Parasitology Section, Department of Medicine and Animal Health, Facultad de Veterinaria, Universidad de Extremadura, Cáceres, Spain
I. NAVARRETE
Affiliation:
Parasitology Section, Department of Medicine and Animal Health, Facultad de Veterinaria, Universidad de Extremadura, Cáceres, Spain
Get access Rights & Permissions [Opens in a new window]

Abstract

A total of 35 pigs aged 15 weeks old, and 21 pigs aged 8 weeks old were divided into 7 groups. Groups 1 and 2 were uninfected and challenge control groups, respectively. Groups 3 and 4 were infected weekly with 6 increasing doses of Ascaris suum eggs, and group 4 was additionally treated with pyrantel. Groups 5, 6, and 7 were immunized weekly with the 14, 42, or 97 kDa fractions from adult worms, respectively. Animals of groups 2–7 were challenged with 10 000 A. suum eggs 7 days after the last infection/immunization. Serum was sampled weekly and specific IgG1, IgG2, and IgM responses were measured. Pigs of groups 5, 6, and 7 showed high IgG1 and IgG2 responses especially against adult worms antigens, while infected groups had high IgG1 and IgM responses, especially against larva. The IgG1 responses were negatively correlated to the numbers of larvae in the lungs, and positively associated with the liver white spot numbers. There was a positive correlation between IgG2 and the numbers of white spots and lung larvae, while IgM was negatively correlated with these parasitological measures. These findings are discussed and it is suggested that acquired resistance against A. suum larvae is correlated with the induction of IgG1 and IgM, and not with IgG2, and that future vaccination protocols may focus on inducing the Th2 activity.

Keywords

Ascaris suumantigensIgG1IgG2IgMpigs
Type
Research Article
Information
Parasitology , Volume 127 , Issue 3 , September 2003 , pp. 291 - 298
Copyright
© 2003 Cambridge University Press

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

ABBAS, A. K., LICHTMAN, A. H. & POBER, J. S. (1995). Inmunología Celular y Molecular, 4th Edn. Interamericana, McGraw-Hill, New York.
BØGH, H. O., ERIKSEN, L., LAWSON, L. G. & LIND, P. (1994). Evaluation of an enzyme-linked immunosorbent assay and a histamine release test system for the detection of pigs naturally infected with Ascaris suum. Preventive Veterinary Medicine 21, 201–214.CrossRefGoogle Scholar
COFFMAN, R. L. & CARTY, J. (1986). A T cell activity that enhances polyclonal IgE production and its inhibition by interferon-gamma. Journal of Immunology 136, 949–954.Google Scholar
CRANDALL, C. A. & CRANDALL, R. B. (1971). Ascaris suum: immunoglobulin responses in mice. Experimental Parasitology 30, 126–137.CrossRefGoogle Scholar
CRAWLEY, A., RAYMOND, C. & WILKIE, B. N. (2003). Control of immunoglobulin isotype production by porcine B-cells cultured with cytokines. Veterinary Immunology and Immunopathology 91, 141–154.CrossRefGoogle Scholar
DAY, R. W. & QUINN, G. P. (1989). Comparisons of treatments after analysis of variance in ecology. Ecological Monographs 59, 433–463.CrossRefGoogle Scholar
ERIKSEN, L., LIND, P., NANSEN, P., ROEPSTORFF, A. & URBAN, J. (1992 a). Resistance to Ascaris suum in parasite naive and naturally exposed growers, finishers and sows. Veterinary Parasitology 41, 137–149.Google Scholar
ERIKSEN, L., NANSEN, P., ROEPSTORFF, A., LIND, P. & NILSSON, O. (1992 b). Response to repeated inoculations with Ascaris suum eggs in pigs during the fattening period. I. Studies on worm population kinetics. Parasitology Research 78, 241–246.Google Scholar
FURESZ, S. E., WILKIE, B. N., MALLARD, B. A., ROSENDAL, S. & MACINNES, J. I. (1998). Anti-haemolysin IgG1 to IgG2 ratios correlates with haemolysin neutralization titres and lung lesion scores in Actinobacillus pleuropneumoniae infected pigs. Vaccine 16, 1971–1975.CrossRefGoogle Scholar
HELWIGH, A. B., CHRISTENSEN, C. M., ROEPSTORFF, A. & NANSEN, P. (1999). Concurrent Ascaris suum and Oesophagostomum dentatum infections in pigs. Veterinary Parasitology 82, 221–234.CrossRefGoogle Scholar
HELWIGH, A. B. & NANSEN, P. (1999). Establishment of Ascaris suum in the pig: Development of immunity following a single primary infection. Acta Veterinaria Scandinavica 40, 121–132.Google Scholar
HILL, D. E., FETTERER, R. H., ROMANOWSKI, R. D. & URBAN, J. F. (1994). The effect of immunisation of pigs with Ascaris suum cuticle components on the development of resistance to parenteral migration during a challenge infection. Veterinary Immunology and Immunopathology 42, 161–169.CrossRefGoogle Scholar
JUNGERSEN, G., FAGERHOLM, H. P., NANSEN, P. & ERIKSEN, L. (1999 a). Development of patent Ascaris suum infections in pigs after intravenous administration of larvae hatched in vitro. Parasitology 119, 503–508.Google Scholar
JUNGERSEN, G., ERIKSEN, L., ROEPSTORFF, A., LIND, P., MEEUSEN, E. N. T. & NANSEN, P. (1999 b). Experimental Ascaris suum infections in the pig: protective memory response after three immunisations and effect to intestinal worm population. Parasite Immunology 21, 619–630.Google Scholar
KELLEY, G. W. & NAYAK, D. P. (1964). Acquired immunity to migrating larvae of Ascaris suum induced in pigs by repeated oral inoculations of infective eggs. Journal of Parasitology 50, 499–503.CrossRefGoogle Scholar
LAEMMLI, U. K. (1970). Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, London 277, 680–685.CrossRefGoogle Scholar
LIND, P., ERIKSEN, L., NANSEN, P., NILSSON, O. & ROEPSTORFF, A. (1993). Response to repeated inoculations with Ascaris suum eggs in pigs during the fattening period. II. Specific IgA, IgG, and IgM antibodies determined by enzyme-linked immunosorbent assay. Parasitology Research 79, 240–244.Google Scholar
LUNNEY, J. K., URBAN, J. F. & JOHNSON, L. A. (1986). Protective immunity to Ascaris suum: analysis of swine peripheral blood subsets using monoclonal antibodies and flow cytometry. Veterinary Parasitology 20, 117–131.CrossRefGoogle Scholar
MOSMANN, T. R. & COFMANN, R. L. (1989). Heterogeneity of cytokine secretion patterns and functions of helper T cells. Advances in Immunology 46, 111–147.CrossRefGoogle Scholar
SERRANO, F. J., REINA, D., FRONTERA, E., NAVARRETE, I. & ROEPSTORFF, A. (2001). Resistance against migrating Ascaris suum larvae in pigs immunised with adult worm antigens. Parasitology 105, 111–115.Google Scholar
SNAPPER, C. M., PESCHEL, C. & PAUL, W. E. (1988). Interferon-γ stimulates IgG2a secretion by murine B-cells stimulated with bacterial lipopolysaccharide. Journal of Immunology 140, 2121–2127.Google Scholar
STANKIEWICZ, M., JONAS, W. & FROE, D. L. (1992). Patent infections of Ascaris suum in pigs: effect of previous exposure to multiple, high doses of eggs and various treatment regimes. International Journal for Parasitology 22, 597–601.CrossRefGoogle Scholar
STANKIEWICZ, M. & FROE, D. L. (1995). Unabbreviated Ascaris suum immunising infections of piglets lead to immunosupression and increased numbers of intestinal parasites. Acta Parasitologica 40, 152–155.Google Scholar
URBAN, J. F., ALIZADEH, H. & ROMANOWSKI, R. D. (1988). Ascaris suum: Development of intestinal immunity to infective second-stage larvae in swine. Experimental Parasitology 66, 66–77.CrossRefGoogle Scholar
URBAN, J. F. & ROMANOWSKI, R. D. (1985). Ascaris suum: protective immunity in pigs immunised with products from eggs and larvae. Experimental Parasitology 60, 245–254.CrossRefGoogle Scholar
URBAN, J. F. & TROMBA, F. G. (1982). Development of immune responsiveness to Ascaris suum antigens in pigs vaccinated with ultraviolet-attenuated eggs. Veterinary Immunology and Immunopathology 3, 399–409.CrossRefGoogle Scholar