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The specific immunoglobulin response in cattle to Theileria parva (Muguga) infection

Published online by Cambridge University Press:  06 April 2009

G. G. Wagner ,
W. P. H. Duffus ,
C. Akwabi ,
M. J. Burridge  and
M. Lule
G. G. Wagner
Affiliation:
From the United States Department of Agriculture Co-operative Research Project, c/oEast African Veterinary Research Organization, Muguga, P.O. Box 32, Kikuyu, Kenya; and the UNDP/FAO Special Fund Project (Ken 22), Research on Tickborne Cattle Diseases and Tick Control, c/oVeterinary Research Laboratory, P.O. Kabete; and the UNDP/FAO Immunological Research on Tick-borne Cattle Diseases and Tick Control Project (AFR REG 77), c/oEast African Veterinary Research Organization, Muguga, P.O. Box 32, Kikuyu, Kenya
W. P. H. Duffus
Affiliation:
From the United States Department of Agriculture Co-operative Research Project, c/oEast African Veterinary Research Organization, Muguga, P.O. Box 32, Kikuyu, Kenya; and the UNDP/FAO Special Fund Project (Ken 22), Research on Tickborne Cattle Diseases and Tick Control, c/oVeterinary Research Laboratory, P.O. Kabete; and the UNDP/FAO Immunological Research on Tick-borne Cattle Diseases and Tick Control Project (AFR REG 77), c/oEast African Veterinary Research Organization, Muguga, P.O. Box 32, Kikuyu, Kenya
C. Akwabi
Affiliation:
From the United States Department of Agriculture Co-operative Research Project, c/oEast African Veterinary Research Organization, Muguga, P.O. Box 32, Kikuyu, Kenya; and the UNDP/FAO Special Fund Project (Ken 22), Research on Tickborne Cattle Diseases and Tick Control, c/oVeterinary Research Laboratory, P.O. Kabete; and the UNDP/FAO Immunological Research on Tick-borne Cattle Diseases and Tick Control Project (AFR REG 77), c/oEast African Veterinary Research Organization, Muguga, P.O. Box 32, Kikuyu, Kenya
M. J. Burridge
Affiliation:
From the United States Department of Agriculture Co-operative Research Project, c/oEast African Veterinary Research Organization, Muguga, P.O. Box 32, Kikuyu, Kenya; and the UNDP/FAO Special Fund Project (Ken 22), Research on Tickborne Cattle Diseases and Tick Control, c/oVeterinary Research Laboratory, P.O. Kabete; and the UNDP/FAO Immunological Research on Tick-borne Cattle Diseases and Tick Control Project (AFR REG 77), c/oEast African Veterinary Research Organization, Muguga, P.O. Box 32, Kikuyu, Kenya
M. Lule
Affiliation:
From the United States Department of Agriculture Co-operative Research Project, c/oEast African Veterinary Research Organization, Muguga, P.O. Box 32, Kikuyu, Kenya; and the UNDP/FAO Special Fund Project (Ken 22), Research on Tickborne Cattle Diseases and Tick Control, c/oVeterinary Research Laboratory, P.O. Kabete; and the UNDP/FAO Immunological Research on Tick-borne Cattle Diseases and Tick Control Project (AFR REG 77), c/oEast African Veterinary Research Organization, Muguga, P.O. Box 32, Kikuyu, Kenya
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Extract

Cattle were inoculated with dilutions of Theileria parva (Muguga) stabilate without concurrent antibiotic treatment. The dilutions were selected to induce severe East Coast fever infections, which in some cases would lead to death. Serum samples were collected and the complement fixation (CF), indirect haemagglutination (IHA) and indirect immunofluorescence (IFA) tests were used to detect T. parva serum antibodies. Sera were fractionated on Sephadex G200 to determine the activities of specific immunoglobulin fractions. Sequential production of IgM antibody followed by 7SIg antibody was demonstrated by the IHA and CF tests. All IFA activity was restricted to the 7SIg fraction. Serologic reactions in the IHA test were detected as early as 4 days after inoculation of the stabilate, CF activity after 8 days and IFA activity after 21 days.

Type
Research Article
Information
Parasitology , Volume 70 , Issue 1 , February 1975 , pp. 95 - 102
Copyright
Copyright © Cambridge University Press 1975

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References

Burridge, M. J., & Kimber, C. D., (1973). Duration of serological response to the indirect fluorescent antibody test of cattle recovered from Theileria parva infection. Research in Veterinary Science 14, 270–1.CrossRefGoogle Scholar
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Duffus, W. P. H., & Wagner, G. G., (1974). The specific immunoglobulin response in cattle immunized with Theileria parva (Muguga) stabilate. Parasitology 69, 31–41.CrossRefGoogle ScholarPubMed
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