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The specific immunoglobulin response in cattle immunized with isolated Theileria parva antigens

Published online by Cambridge University Press:  06 April 2009

G. G. Wagner ,
W. P. H. Duffus  and
M. J. Burridge
G. G. Wagner
Affiliation:
East African Veterinary Research Organization, Muguga, P.O. Box 32, Kikuyu, Kenya and Veterinary Research Laboratory, P.O. Kabete, Kenya
W. P. H. Duffus
Affiliation:
East African Veterinary Research Organization, Muguga, P.O. Box 32, Kikuyu, Kenya and Veterinary Research Laboratory, P.O. Kabete, Kenya
M. J. Burridge
Affiliation:
East African Veterinary Research Organization, Muguga, P.O. Box 32, Kikuyu, Kenya and Veterinary Research Laboratory, P.O. Kabete, Kenya
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Cattle were inoculated with purified Theileria parva piroplasm and/or schizont antigen. Two similar inoculations were given 10 days apart. Serum samples were regularly collected and the indirect haemagglutination (IHA), indirect immunofluorescence (IFA), complement-fixation (CF) and immunodiffusion (ID) tests were used to detect T. parva antibodies. Selected sera were separated by Sephadex G 200 and fractions examined for specific immunoglobulin activity.

With the IHA test specific antibody first appeared 4 days post inoculation (dpi) and reached high titres by 8 dpi. With the IFA and CF tests specific antibody also appeared 4 dpi and reached high titres by 8–14 and 10–14 dpi respectively. Specific ID activity was detected as early as 6 dpi and persisted for the length of the experiment.

On fractionation of the sera both the IHA and CF tests indicated a sequential production of T. parva IgM and 7S Ig. However, in cattle inoculated with schizont antigen only, a significant 7S Ig component was not detected by the IHA test until 16 dpi, but was demonstrated by the CF test 7 dpi. The IFA test, however, detected anti T. parva activity almost exclusively in the 7S Ig fractions. All cattle were challenged 35–42 dpi with infective T. parva stabilate and all cattle proved fully susceptible. The lack of protection by high titres of specific T. parva immunoglobulin is discussed, together with the data on the differential immunoglobulin response, in relation to previous results.

Type
Research Article
Information
Parasitology , Volume 69 , Issue 1 , August 1974 , pp. 43 - 53
Copyright
Copyright © Cambridge University Press 1974

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References

REFERENCES

Brocklesby, D. W., Barnett, S. F. & Scott, G. R. (1961). Morbidity and mortality rates in East Coast Fever (Theileria parva infection) and their application to drug screening procedures. British Veterinary Journal 117, 529–31.CrossRefGoogle Scholar
Burridge, M. J. (1971). Application of the indirect fluorescent antibody test in experimental East Coast Fever (Theileria parva infection of cattle). Research in Veterinary Science 12, 338–41.CrossRefGoogle ScholarPubMed
Burridge, M. J. & Kimber, C. D. (1972). The indirect fluorescent antibody test for experimental East Coast Fever (Theileria parva infection of cattle). Evaluation of a cell culture schizont antigen. Research in Veterinary Science 13, 451–5.CrossRefGoogle ScholarPubMed
Burridge, M. J., Kimber, C. D. & Young, A. S. (1973). Use of the indirect fluorescent antibody technique in serological studies of Theileria lawrencei infections in cattle. American Journal of Veterinary Research 34, 897–900.Google ScholarPubMed
Cunningham, M. P., Brown, C. G. D., Burridge, M. J. & Purnell, R. E. (1973). Cryopreservation of infective particles of Theileria parva. International Journal of Parasitology. (In the Press.)CrossRefGoogle ScholarPubMed
Duffus, W. P. H. & Wagner, G. G. (1973). Immunochemical studies on East Coast Fever. III. Development of an indirect haemagglutination assay using Theileria parva piroplasm antigen. (Submitted for publication.)Google Scholar
Duffus, W. P. H. & Wagner, G. G. (1974). The specific immunoglobulin response in cattle immunized with Theileria parva (Muguga) stabilate. Parasitology 69, 31–41.CrossRefGoogle ScholarPubMed
Hullinger, L., Brown, C. G. D. & Wilde, J. K. H. (1965). Theileriosis (T. parva) immune mechanism investigated in vitro. In Abstracts of the Second International Conference on Protozoology, p. 37. London.Google Scholar
Kabat, E. A. (1964). Protein estimation using the Folin-Ciocalteau phenol reagent. In Experimental Immunochemistry (ed. Kabat, E. A. and Mayer, M. M.), p. 556. Springfield, Illinois: Charles H. Thomas.Google Scholar
Lumsden, W. H. R. & Hardy, E. J. C. (1965). Nomenclature of living parasite material. Nature, London 205, 1032.CrossRefGoogle Scholar
Malmquist, W. A., Nyindo, M. B. A. & Brown, C. G. D. (1970). East Coast Fever: cultivation in vitro of bovine spleen cell lines infected and transformed by Theileria parva. Tropical Animal Health and Production 2, 139–45.CrossRefGoogle Scholar
Purnell, R. E., Brown, C. G. D., Cunningham, M. P., Burridge, M. J., Kirimi, I. M. & Ledger, M. A. (1973). East Coast Fever: correlation between the histology and infectivity of Theileria parva developing in the tick vector. Parasitology 66, 539–44.CrossRefGoogle Scholar
Wagner, G. G., Brown, C. G. D., Duffus, W. P. H., Kimber, C. D., Crawford, J. E. & Lule, M. (1973). Immunochemical studies on East Coast Fever. I. Partial segregation and characterization of the Theileria parva schizont antigen. (Submitted for publication.)Google Scholar
Wagner, G. G., Duffus, W. P. H., Kimber, C. D. & Lule, M. (1973). Immunochemical studies on East Coast Fever. II. Partial segregation and characterization of the Theileria parva piroplasm antigen. (Submitted for publication.)Google Scholar