Hostname: page-component-586b7cd67f-t8hqh Total loading time: 0 Render date: 2024-11-23T05:30:52.671Z Has data issue: false hasContentIssue false
  • English
  • Français

Simple and reliable preparation of immunodiagnostic antigens for Taenia solium cysticercosis

Published online by Cambridge University Press:  21 June 2013

YASUHITO SAKO ,
SONOYO ITOH ,
MUNEHIRO OKAMOTO ,
KAZUHIRO NAKAYA  and
AKIRA ITO
YASUHITO SAKO*
Affiliation:
Department of Parasitology, Asahikawa Medical University, Asahikawa, Hokkaido, Japan
SONOYO ITOH
Affiliation:
Department of Parasitology, Asahikawa Medical University, Asahikawa, Hokkaido, Japan
MUNEHIRO OKAMOTO
Affiliation:
Center for Human Evolution Modeling Research, Primate Research Institute, Kyoto University, Inuyama, Aichi, Japan
KAZUHIRO NAKAYA
Affiliation:
Animal Laboratory for Medical Research, Center for Advanced Research and Education, Asahikawa Medical University, Asahikawa, Hokkaido, Japan
AKIRA ITO
Affiliation:
Department of Parasitology, Asahikawa Medical University, Asahikawa, Hokkaido, Japan
*
*Corresponding author: Department of Parasitology, Asahikawa Medical University, Midorigaoka Higashi 2-1-1-1, Asahikawa, 078-8510 Hokkaido, Japan. Tel: +81-166-2422. Fax: +81-166-68-2429. E-mail: [email protected]
Get access Rights & Permissions [Opens in a new window]

Summary

Cysticercosis caused by infection with the larval stage of Taenia solium is an important cause of neurological disease worldwide and immunodiagnosis is important for the control and elimination of cysticercosis. In the present study, we established a simple and reliable preparation of immunodiagnostic low-molecular-weight antigens (LMWAgs) from T. solium cyst fluids by a cation-exchange chromatography (CEC). Banding patterns of LMWAgs on SDS-PAGE were different between isolates from Ecuador and China. All cysticercosis patient sera and some echinococcosis patient sera recognized both LMWAgs by enzyme-linked immunosorbent assay (ELISA), but sera from healthy persons were not positive. There was no statistical difference in immunodiagnostic performance of LMWAgs prepared from different geographical isolates. These results indicated that these novel immunodiagnostic antigen preparations could contribute the control and prevention of cysticercosis in endemic areas, especially developing countries.

Keywords

Taenia soliumcysticercosisimmunodiagnostic antigenslow-molecular-weight antigenscation-exchange chromatographysimple preparation
Type
Research Article
Information
Parasitology , Volume 140 , Special Issue 13: Control of cestode zoonoses in Asia: role of basic and applied science , November 2013 , pp. 1589 - 1594
Copyright
Copyright © Cambridge University Press 2013 

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

Chung, J. Y., Bahk, Y. Y., Huh, S., Kang, S. Y., Kong, Y. and Cho, S. Y. (1999). A recombinant 10-kDa protein of Taenia solium metacestodes specific to active neurocysticercosis. Journal of Infectious Diseases 180, 1307–1315.CrossRefGoogle ScholarPubMed
Greene, R. M., Hancock, K., Wilkins, P. P. and Tsang, V. C. (2000). Taenia solium: molecular cloning and serologic evaluation of 14- and 18-kDa related, diagnostic antigens. Journal of Parasitology 86, 1001–1007.CrossRefGoogle ScholarPubMed
Hancock, K., Khan, A., Williams, F. B., Yushak, M. L., Pattabhi, S., Noh, J. and Tsang, V. C. (2003). Characterization of the 8-kilodalton antigens of Taenia solium metacestodes and evaluation of their use in an enzyme-linked immunosorbent assay for serodiagnosis. Journal of Clinical Microbiology 41, 2577–2586.CrossRefGoogle Scholar
Ito, A. and Craig, P. S. (2003). Immunodiagnostic and molecular approaches for the detection of taeniid cestode infections. Trends in Parasitology 19, 377–381.CrossRefGoogle ScholarPubMed
Ito, A., Nakao, M., Ito, Y., Yuzawa, I., Morishima, H., Kawano, N. and Fujii, K. (1999). Neurocysticercosis case with a single cyst in the brain showing dramatic drop in specific antibody titers within 1 year after curative surgical resection. Parasitology International 48, 95–99.CrossRefGoogle ScholarPubMed
Ito, A., Plancarte, A., Ma, L., Kong, Y., Flisser, A., Cho, S. Y., Liu, Y. H., Kamhawi, S., Lightowlers, M. W. and Schantz, P. M. (1998). Novel antigens for neurocysticercosis: simple method for preparation and evaluation for serodiagnosis. American Journal of Tropical Medicine and Hygiene 59, 291–294.CrossRefGoogle ScholarPubMed
Lee, E. G., Bae, Y. A., Kim, S. H., Diaz-Camacho, S. P., Nawa, Y. and Kong, Y. (2010). Serodiagnostic reliability of single-step enriched low-molecular weight proteins of Taenia solium metacestode of American and Asian isolates. Transactions of the Royal Society of Tropical Medicine and Hygiene 104, 676–683.CrossRefGoogle ScholarPubMed
Mahanty, S. and Garcia, H. H. (2010). Cysticercosis and neurocysticercosis as pathogens affecting the nervous system. Progress in Neurobiology 91, 172–184.CrossRefGoogle ScholarPubMed
Mamuti, W., Yamasaki, H., Sako, Y., Nakao, M., Xiao, N., Nakaya, K., Sato, N., Vuitton, D. A., Piarroux, R., Lightowlers, M. W., Craig, P. S. and Ito, A. (2004). Molecular cloning, expression, and serological evaluation of an 8-kilodalton subunit of antigen B from Echinococcus multilocularis. Journal of Clinical Microbiology 42, 1082–1088.CrossRefGoogle ScholarPubMed
Nakao, M., Okamoto, M., Sako, Y., Yamasaki, H., Nakaya, K. and Ito, A. (2002). A phylogenetic hypothesis for the distribution of two genotypes of the pig tapeworm Taenia solium worldwide. Parasitology 124, 657–662.CrossRefGoogle ScholarPubMed
Oriol, R., Williams, J. F., Perez Esandi, M. V. and Oriol, C. (1971). Purification of lipoprotein antigens of Echinococcus granulosus from sheep hydatid fluid. American Journal of Tropical Medicine and Hygiene 20, 569–574.CrossRefGoogle ScholarPubMed
Parkhouse, R. M. and Harrison, L. J. (1987). Cyst fluid and surface associated glycoprotein antigens of Taenia sp. metacestodes. Parasite Immunology 9, 263–268.CrossRefGoogle ScholarPubMed
Sako, Y., Nakao, M., Ikejima, T., Piao, X. Z., Nakaya, K. and Ito, A. (2000). Molecular characterization and diagnostic value of Taenia solium low-molecular-weight antigen genes. Journal of Clinical Microbiology 38, 4439–4444.CrossRefGoogle ScholarPubMed
Sako, Y., Nakao, M., Nakaya, K., Yamasaki, H., Gottstein, B., Lightowers, M. W., Schantz, P. M. and Ito, A. (2002). Alveolar echinococcosis: characterization of diagnostic antigen Em18 and serological evaluation of recombinant Em18. Journal of Clinical Microbiology 40, 2760–2765.CrossRefGoogle ScholarPubMed
Sato, M. O., Sako, Y., Nakao, M., Yamasaki, H., Nakaya, K. and Ito, A. (2006). Evaluation of purified Taenia solium glycoproteins and recombinant antigens in the serologic detection of human and swine cysticercosis. Journal of Infectious Diseases 194, 1783–1790.CrossRefGoogle ScholarPubMed
Sato, M. O., Yamasaki, H., Sako, Y., Nakao, M., Nakaya, K., Plancarte, A., Kassuku, A. A., Dorny, P., Geerts, S., Benitez-Ortiz, W., Hashiguchi, Y. and Ito, A. (2003). Evaluation of tongue inspection and serology for diagnosis of Taenia solium cysticercosis in swine: usefulness of ELISA using purified glycoproteins and recombinant antigen. Veterinary Parasitology 111, 309–322.CrossRefGoogle ScholarPubMed
Tsang, V. C., Brand, J. A. and Boyer, A. E. (1989). An enzyme-linked immunoelectrotransfer blot assay and glycoprotein antigens for diagnosing human cysticercosis (Taenia solium). Journal of Infectious Diseases 159, 50–59.CrossRefGoogle ScholarPubMed