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Schistosomula, pre-adults and adults of Schistosoma mansoni ingest fluorescence-labelled albumin in vitro and in vivo: implication for a drug-targeting model

Published online by Cambridge University Press:  26 May 2010

M. C. HOLTFRETER ,
M. LOEBERMANN ,
E. FREI ,
D. RIEBOLD ,
D. WOLFF ,
G. HARTUNG ,
R. KINZELBACH  and
E. C. REISINGER
M. C. HOLTFRETER
Affiliation:
Division of Tropical Medicine and Infectious Diseases, Department of Internal Medicine, University of Rostock, Ernst-Heydemann Strasse 6, 18057 Rostock, Germany
M. LOEBERMANN
Affiliation:
Division of Tropical Medicine and Infectious Diseases, Department of Internal Medicine, University of Rostock, Ernst-Heydemann Strasse 6, 18057 Rostock, Germany
E. FREI
Affiliation:
Division of Molecular Toxicology, German Cancer Research Center, Im Neuenheimer Feld 280, 69120 Heidelberg, Germany
D. RIEBOLD
Affiliation:
Division of Tropical Medicine and Infectious Diseases, Department of Internal Medicine, University of Rostock, Ernst-Heydemann Strasse 6, 18057 Rostock, Germany
D. WOLFF
Affiliation:
Department of Haematology/Oncology, University Medical Center, Franz-Josef-Strauss-Allee 11, 93053 Regensburg, Germany
G. HARTUNG
Affiliation:
Department for Medical Oncology/Hematology, Klinikum Oldenburg, Rahel-Straus-Strasse 10, 26133 Oldenburg, Germany
R. KINZELBACH
Affiliation:
Department of General and Systematic Zoology, Institute for Biosciences, University of Rostock, Universitätsplatz 2, 18055 Rostock, Germany
E. C. REISINGER*
Affiliation:
Division of Tropical Medicine and Infectious Diseases, Department of Internal Medicine, University of Rostock, Ernst-Heydemann Strasse 6, 18057 Rostock, Germany
*
*Corresponding author: Division of Tropical Medicine and Infectious Diseases, Department of Internal Medicine, University of Rostock Medical School, Ernst-Heydemann-Strasse 6, D-18057 Rostock, Germany. Tel: 0049 381 4947510. Fax: 0049 381 494 7509. E-mail: [email protected]
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Summary

Objective. Bilharziosis is one of the most important helminthal infections in humans and is caused by blood flukes of the genus Schistosoma. Three different life stages of the parasite occur within the mammalian host: schistosomula located in the skin, pre-adults located in the lung and adult worms located in the portal venous system. Erythrocytes are a major source of nutrient supply for adults. However, sources of nutrition for the developing stages are still unclear. Methods. To investigate whether schistosomula, pre-adults and adults of Schistosoma mansoni ingest human serum albumin (HSA) in vitro, these life stages were incubated with aminofluorescein-labelled human serum albumin (Afl-HSA) for 5 h. To test the uptake of albumin in vivo, the albumin conjugate was given intravenously to S. mansoni infected NMRI mice 24 h before harvesting the 3 life stages. Results. In comparison to the control group schistosomula, pre-adults, and adults showed an accumulation of Afl-HSA within the oesophagus and intestinal caecum in vitro and in vivo. Conclusion. Our findings suggest that albumin seems to be a major source of energy supply for the early schistosomal life stages and an additive energy support for adult worms. Since albumin has been used successfully as a drug carrier for chemotherapeutic substances against malignant disorders, further studies will focus on albumin as a carrier for anthelminthics in a drug-targeting model.

Keywords

Schistosoma mansonischistosomulapre-adultsadultsingestionhuman serum albuminaminofluoresceindrug targeting
Type
Research Article
Information
Parasitology , Volume 137 , Issue 11 , September 2010 , pp. 1645 - 1652
Copyright
Copyright © Cambridge University Press 2010

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