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Schistosoma mansoni: evidence for a role of serum factors in protecting artificially transformed schistosomula against antibody-mediated killing in vitro

Published online by Cambridge University Press:  06 April 2009

C. A. P. Tavares ,
Rita C. Soares ,
P. M. Z. Coelho  and
G. Gazzinelli
C. A. P. Tavares
Affiliation:
Departamento de Bioquímica-Immunologia, Instituto de Ciäcias Biológicas da UFMG, C.P. 2486, 3.000 Belo Horizonte, Brasil
Rita C. Soares
Affiliation:
Departamento de Bioquímica-Immunologia, Instituto de Ciäcias Biológicas da UFMG, C.P. 2486, 3.000 Belo Horizonte, Brasil
P. M. Z. Coelho
Affiliation:
Departamento de Bioquímica-Immunologia, Instituto de Ciäcias Biológicas da UFMG, C.P. 2486, 3.000 Belo Horizonte, Brasil
G. Gazzinelli
Affiliation:
Departamento de Bioquímica-Immunologia, Instituto de Ciäcias Biológicas da UFMG, C.P. 2486, 3.000 Belo Horizonte, Brasil
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Summary

Artificially transformed schistosomula of Schistosoma mansoni develop a consistent but small protection against the lethal effects of antibody plus complement when cultured for 24 h in a chemically defined medium. In contrast, they become rapidly resistant to antibody plus complement, when cultured in the presence of a complex medium consisting of equal parts of heat-inactivated rabbit serum and Earle's/lactalbumin or in defined medium supplemented with small amounts of heat-inactivated rabbit serum. Sephadex G-200 gel filtration revealed that the protective factor in rabbit serum is a macromolecule with a molecular weight between 7 and 19S. Parasites cultured at 10 °C or in the presence of 200 μg of puromycin show less serum-induced protection against the lethal effects of antibody plus complement than do controls.

Type
Research Article
Information
Parasitology , Volume 77 , Issue 2 , October 1978 , pp. 225 - 233
Copyright
Copyright © Cambridge University Press 1978

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References

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