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Schistosoma mansoni: effect of maintenance in vitro on the physiology and biochemistry of adult worms

Published online by Cambridge University Press:  06 April 2009

J. G. Mercer  and
L. H. Chappell
J. G. Mercer
Affiliation:
Department of Zoology, University of Aberdeen, Tillydrone Avenue, Aberdeen, Scotland AB9 2TN
L. H. Chappell
Affiliation:
Department of Zoology, University of Aberdeen, Tillydrone Avenue, Aberdeen, Scotland AB9 2TN
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Extract

In Medium 199 supplemented with calf serum (+ 10%), adult Schistosoma mansoni produced a mean total of 400 eggs/worm pair during maintenance for 10 days in vitro. During the period of egg deposition in vitro, the dry weight of worm pairs decreased by 35%. Biomass decreases were proportionally greater in female worms than in males. Declines in the dry weight of female worms were mainly due to decreases in protein content, while biomass decreases in males resulted from losses of both protein and glycogen. Glucose was depleted from the maintenance medium at a rate of approximately 200 μg/worm pair/day during each of the first 3 days in vitro, while concentrations of glucose in the medium of above 1 g/litre did not affect the degree of glycogen depletion observed in adult S. mansoni during culture for 24 h. The contribution of the loss of schistosome gut contents in vitro to the observed changes is discussed. The work described represents part of an attempt at logical design of in vitro culture.

Type
Research Article
Information
Parasitology , Volume 90 , Issue 2 , April 1985 , pp. 339 - 349
Copyright
Copyright © Cambridge University Press 1985

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