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Schistosoma mansoni: circulating and pulmonary leucocyte responses related to the induction of protective immunity in mice by irradiated parasites

Published online by Cambridge University Press:  06 April 2009

Essie N. Menson ,
Patricia S. Coulson  and
R. A. Wilson
Essie N. Menson
Affiliation:
Department of Biology, University of York, York YO1 5DD
Patricia S. Coulson
Affiliation:
Department of Biology, University of York, York YO1 5DD
R. A. Wilson
Affiliation:
Department of Biology, University of York, York YO1 5DD
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Summary

The leucocyte responses in peripheral blood and pulmonary airways are described following vaccination of mice with radiation-attenuated parasites, and subsequent challenge with normal parasites. Percutaneous vaccination stimulated a large and sustained expansion of the circulating lymphocyte pool, more marked than after intradermal vaccination with lung schistosomula which induced comparable levels of resistance. Macrophages and lymphocytes infiltrated the pulmonary airways in response to vaccination by both routes, the lymphocytes being particularly abundant after intradermal vaccination. Exposure of mice to an equivalent number of normal cercariae induced an earlier lymphocytosis of short duration; far fewer macrophages and lymphocytes infiltrated the lungs than after vaccination. An intense but transient pulmonary eosinophilia peaked at 3 weeks after primary exposure to either normal or attenuated parasites. Percutaneous challenge of vaccinated mice elicited higher levels of circulating lymphocytes than challenge of unsensitized controls. However, whilst leucocyte numbers of all cell types were still elevated in the airways at challenge as a consequence of vaccination, no further cellular recruitment was observed coincident with parasite elimination. Our data are compatible with the hypothesis that the mechanism of immunity in once-vaccinated mice involves a T lymphocyte-macrophage interaction triggered by antigen release from lung schistosomula.

Keywords

Schistosoma mansonileucocytesprotective immunityirradiation
Type
Research Article
Information
Parasitology , Volume 98 , Issue 1 , February 1989 , pp. 43 - 55
Copyright
Copyright © Cambridge University Press 1989

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