Hostname: page-component-586b7cd67f-rcrh6 Total loading time: 0 Render date: 2024-12-01T00:27:19.906Z Has data issue: false hasContentIssue false
  • English
  • Français

Resistance against Schistosoma mansoni induced by highly irradiated infections: studies on species specificity of immunization and attempts to transfer resistance

Published online by Cambridge University Press:  06 April 2009

Q. D. Bickle ,
B. J. Andrews ,
M. J. Doenhoff ,
M. J. Ford  and
M. G. Taylor
Q. D. Bickle
Affiliation:
Department of Medical Helminthology, Winches Farm Field Station, London School of Hygiene and Tropical Medicine, 395 Hatfield Road, St Albans AL4 OXQ
B. J. Andrews
Affiliation:
Department of Medical Helminthology, Winches Farm Field Station, London School of Hygiene and Tropical Medicine, 395 Hatfield Road, St Albans AL4 OXQ
M. J. Doenhoff
Affiliation:
Department of Medical Helminthology, Winches Farm Field Station, London School of Hygiene and Tropical Medicine, 395 Hatfield Road, St Albans AL4 OXQ
M. J. Ford
Affiliation:
Department of Medical Helminthology, Winches Farm Field Station, London School of Hygiene and Tropical Medicine, 395 Hatfield Road, St Albans AL4 OXQ
M. G. Taylor
Affiliation:
Department of Medical Helminthology, Winches Farm Field Station, London School of Hygiene and Tropical Medicine, 395 Hatfield Road, St Albans AL4 OXQ
Get access Rights & Permissions [Opens in a new window]

Extract

Significant levels of resistance against Schistosoma mansoai challenge were developed by mice exposed to highly irradiated (20 krad.) cercariae of the homologous species (53–67%), whereas vaccination with S. bovis, S. haematobiurn or S.japonicuni failed to confer significant levels of resistance (–5–12%), thus confirming the specificity ofthe immunizing procedure. Attempts to transfer resistance to naive recipients by injection of serum and of spleen or lymph node cells from donor mice vaccinated with highly irradiated cercariae were largely unsuccessful. However, significant levels of resistance could be transferred to mice by injection of serum from rabbits exposed to irradiated cercariae. Comparable levels of resistance were conferred by injection of serum at the time of challenge (34–69%) or 5–6 days later (31–56%). Tn contrast, sera from rabbits injected with solubleegg antigen or homogenized cercariae failed to confer protection upon recipient mice. Sera from vaccinated mice, vaccinated rabbits and antigen-injected rabbits all caused cell adherence to skin-transformed schistosomula hut neither the level of adherence nor the serum titre correlated with the ability to confer protection to mice.

Type
Research Article
Information
Parasitology , Volume 90 , Issue 2 , April 1985 , pp. 301 - 312
Copyright
Copyright © Cambridge University Press 1985

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

Bickle, Q. D. & Ford, M. J. (1982). Studies on the surface antigenicity and susceptibility to antibody-dependent killing of developing schistosomula using sera from chronically infected mice and mice vaccinated with irradiated cercariae. Journal of Immunology 128, 101–6.Google ScholarPubMed
Bickle, Q. D., Ford, M. J. & Andrews, B. J. (1983). Studies on the development of antischistosomula surface antibodies by mice exposed to irradiated cercaria.e, adults and/or eggs of S. mansoni. Parasite Immunology 5, 499–511.CrossRefGoogle ScholarPubMed
Bickle, Q. P., Taylor, M. G., Doenhoff, M. J. & Nelson, G. S. (1979 a). Immunization of mice with gamma-irradiated, intramuscularly injected schistosomula of Schistosoma mansoni. Parasitology 79, 209–22.CrossRefGoogle ScholarPubMed
Bickle, Q. D., Taylor, M. G., James, E. R., Nelson, G. S., Hussein, M. F., Andrews, B. J., Dobinson, A. R. & Marshall, T. F. De C. (1979 b). Further observations on immunization of sheep against Schistosoma maltheei and S. bovis using irradiation-attenuated schistosomula of homologous and heterologous species. Parasitology 78, 185–93.CrossRefGoogle ScholarPubMed
Cheever, A. W., Hieny, S., Duvall, R. H. & Sher, A. (1983). Lack of resistance to Schistosoma japonicum in mice immunized with irradiated S. mansoni cercariae. Transactions of the Royal Society of Tropical Medicine and Hygiene 77, 812–15.CrossRefGoogle ScholarPubMed
Dean, D. A. (1983). A Review. Schistosoma and related genera: acquired resistance in mice. Experimental Parasitology 55, 1–104.CrossRefGoogle ScholarPubMed
Dean, P. A., Bukowski, M. A. & Clarke, S. S. (1981). Attempts to transfer the resistance of Schistosoma mansoni-infected and irradiated cercaria-immunized mice by means of paraboisis. American Journal of Tropical Medicine and Hygiene 30, 113–20.CrossRefGoogle Scholar
Doenhoff, M. J., Leuchars, E. & Rees, C. T. (1976). The mitotic activity of, and antibody production by, sensitized lymphocytes quantified aftertransfer into irradiated recipients. Immunology 31, 205–16.Google Scholar
Doenhoff, M. J. & Long, E. (1979). Factors affecting the acquisition of resistance against Schistosoma mansoni in the mouse. IV. The inability of T-cell-deprived mice to resist re-infection, and other in vivo studies on the mechanisms of resistance. Parasitology 78, 171–83.CrossRefGoogle ScholarPubMed
Doenhoff, M. J., Pearson, S., Dunne, P. W., Bickle, Q., Lucas, S., Bain, J. & Hassounah, O. (1981). Immunological control of hepatotoxicity and parasite egg excretion in Schistosoma mansoni infections: stage specificity of the reactivity of immune sera in T-cell deprived mice. Transactions of the Royal Society of Tropical Medicine and Hygiene 75, 41–53.CrossRefGoogle ScholarPubMed
Ford, M. J., Bickle, Q. D. & Taylor, M. G. (1984). Immunization of rats against Schistosoma mansoni using irradiated cercariae, lung schistosomula and liver-stage worms. Parasilology 89, 327–44.CrossRefGoogle ScholarPubMed
Ford, M. J., Bickle, Q. P., Taylor, M. G. & Andrews, B. J. (1984). Passive transfer of resistance and the site of immune-dependent elimination of the challenge infection in rats vaccinated with highly irradiated cercariae of Schistosoma mansoni. Parasitology 89, 461–82.CrossRefGoogle ScholarPubMed
Hsü, S. Y. L., Hsü, H. F. & Burmeister, L. F. (1981). Schistosorna mansoni: vaccination of mice with highly X-irradiated cercariae. Experimental Parasitology 52, 91–104.CrossRefGoogle Scholar
Hsü, H. F., Hsü, S. Y. L. & Osborne, J. W. (1963). Further studies on rhesus monkeys immunized against Schislosoma japonicum by administration of X-irradiated cercariae. Zeitschrift für Tropen medizin und Parasitologie 14, 40 1–12.Google Scholar
Mastin, A. J., Bickle, Q. D. & Wilson, R. A. (1983). Schistosorna mansoni: migration and attrition of irradiated and challenge schistosomula in the mouse. Parasitology 87, 87–102.CrossRefGoogle Scholar
Minard, P., Dean, D. A., Jacobson, R. H., Vannier, W. E. & Murrell, K. P. (1978). Immunization of mice with cobalt-60 irradiated Schistosoma mansoni cercariae. American Journal of Tropical Medicine and Hygiene 27, 76–86.CrossRefGoogle ScholarPubMed
Murare, H. M. (1983). Studies on the host–parasite relationship of Schistosoma bovis in the mouse. Ph.D. thesis, University of London.Google Scholar
Murrell, K. D., Clarke, S., Dean, D. A. & Vannier, W. E. (1979). Influence of mouse strain on induction of resistance with irradiated Schistosoma mansoni cercariae. Journal of Parasitology 65, 829–31.CrossRefGoogle ScholarPubMed
Sher, A., Hieny, S., James, S. L. & Asofsky, R. (1982). Mechanisms of protective immunity against Schistosoma mansoni infection in mice vaccinated with irradiated cercariae. Journal of Immunology 128, 1800–84.Google ScholarPubMed
Stek, M., Minard, P., Dean, D. A. & Hall, J. E. (1981). Immunization of baboons with Schistosoma mansoni cercariae attenuated by gamma irradiation. Science 212, 1518–20.CrossRefGoogle Scholar
Taylor, M. G. & Bickle, Q. D. (1980). Progress in the development of radiation-attenuated vaccines for schistosomiasis. Inlernational Journal of Nuclear Medicine and Biology 7, 97–103.CrossRefGoogle ScholarPubMed
Taylor, M. G., James, E. R., Bickle, Q. D., Doenhoff, M. J., Nelson, G. S., Hussein, M. F. & Bushara, H. O. (1977). Vaccination in schistosomiasis. Les coiloque l'institut national de la santé et de la recherche médicale 72, 291–306.Google Scholar
Zodda, D. M. & Phillips, S. M. (1982). Monoclonal antibody-mediated protection against Schistosoma mansoni infection in mice. Journal of Immunology 129, 2326–8.CrossRefGoogle ScholarPubMed