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Repair of Hymenolepis diminuta after complement-mediated damage

Published online by Cambridge University Press:  06 April 2009

J. Andreassen  and
D. Hoole
J. Andreassen
Affiliation:
Department of Parasitology, Institute of Population Biology, University of Copenhagen, Universitetsparken 15, DK2100, Copenhagen, Denmark
D. Hoole
Affiliation:
Department of Parasitology, Institute of Population Biology, University of Copenhagen, Universitetsparken 15, DK2100, Copenhagen, Denmark
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Summary

Seven and 56-day-old Hymenolepis diminuta were exposed to complement by incubation in 50% normal rat serum (NRS) in modified Hanks' saline. Ultrastructural studies revealed that the scolex/neck region remained relatively intact whilst in the strobila region microthrix denudation and loss of distal cytoplasm were observed. When complement-mediated damaged worms were incubated in vitro in 50% heat-inactivated normal rat serum (hiNRS) plus M199 or implanted into the duodenum of NMRI mice repair occurred, although destrobilated parasites were only found in vivo. The regions undergoing repair contained tegumental protrusions, vesicles, large electron-lucent areas and large quantities of lipid. Microtriches were formed parallel to the parasite surface and were raised into a perpendicular position. It is suggested that the regenerative process exhibited after complement-mediated damage does not mimic totally the embryological development of the surface layer.

Keywords

Hymenolepis diminutacomplement-mediated damagedestrobilationmicrothrix developmentrepair.
Type
Research Article
Information
Parasitology , Volume 99 , Issue 3 , December 1989 , pp. 437 - 443
Copyright
Copyright © Cambridge University Press 1989

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