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Real-time PCR investigation of parasite ecology: in situ determination of oyster parasite Perkinsus marinus transmission dynamics in lower Chesapeake Bay

Published online by Cambridge University Press:  14 February 2006

C. AUDEMARD ,
L. M. RAGONE CALVO ,
K. T. PAYNTER ,
K. S. REECE  and
E. M. BURRESON
C. AUDEMARD
Affiliation:
Virginia Institute of Marine Science, School of Marine Science, College of William and Mary, Gloucester Point, Virginia, USA
L. M. RAGONE CALVO
Affiliation:
Haskin Shellfish Research Laboratory, Institute of Marine and Coastal Sciences, Rutgers University, Port Norris, New Jersey, USA
K. T. PAYNTER
Affiliation:
Department of Biology, University of Maryland, College Park, Maryland, USA
K. S. REECE
Affiliation:
Virginia Institute of Marine Science, School of Marine Science, College of William and Mary, Gloucester Point, Virginia, USA
E. M. BURRESON
Affiliation:
Virginia Institute of Marine Science, School of Marine Science, College of William and Mary, Gloucester Point, Virginia, USA
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Abstract

Perkinsus marinus is a severe pathogen of the oyster Crassostrea virginica on the East Coast of the United States. Transmission dynamics of this parasite were investigated in situ for 2 consecutive years (May through October) at 2 lower Chesapeake Bay sites. Compared to previous studies where seasonal infection patterns in oysters were measured, this study also provided parasite water column abundance data measured using real-time PCR. As previously observed, salinity and temperature modulated parasite transmission dynamics. Using regression analysis, parasite prevalence, oyster mortalities and parasite water column abundance were significantly positively related to salinity. Perkinsus marinus weighted prevalence in wild oysters and parasite water column abundance both were significantly related to temperature, but the responses lagged 1 month behind temperature. Parasite water column abundance was the highest during August (up to 1200 cells/l) and was significantly related to P. marinus weighted prevalence in wild oysters, and to wild oyster mortality suggesting that parasites are released in the environment via both moribund and live hosts (i.e. through feces). Incidence was not significantly related to parasite water column abundance, which seems to indicate the absence of a linear relationship or that infection acquisition is controlled by a more complex set of parameters.

Keywords

Perkinsus marinusCrassostrea virginicatransmission dynamicsenvironmentwaterborneabundancereal-time PCRSYBR Green Ihost-parasite relationships
Type
Research Article
Information
Parasitology , Volume 132 , Issue 6 , June 2006 , pp. 827 - 842
Copyright
2006 Cambridge University Press

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References

REFERENCES

Andrews, J. D. ( 1988). Epizootiology of the disease caused by the oyster pathogen Perkinsus marinus and its effect on the oyster industry. American Fisheries Society Special Publications 18, 47–63.Google Scholar
Andrews, J. D., Haven, D. and Quayle, D. B. ( 1959). Fresh-water kill of oysters (Crassostrea virginica) in James River, Virginia, 1958. Proceedings of the National Shellfisheries Association 49, 29–49.Google Scholar
Andrews, J. D. and Hewatt, W. G. ( 1957). Oyster mortality studies in Virginia. II. The fungus Dermocystidium marinum in oysters in Chesapeake Bay. Ecological Monographs 27, 1–26.Google Scholar
Audemard, C., Reece, K. S. and Burreson, E. M. ( 2004). Real-time PCR for detection and quantification of the protistan parasite Perkinsus marinus in environmental waters. Applied and Environmental Microbiology 70, 6611–6618.CrossRefGoogle Scholar
Bell, A. B. and Ranford-Cartwright, L. C. ( 2002). Real-time quantitative PCR in parasitology. Trends in Parasitology 18, 337–342.CrossRefGoogle Scholar
Brown, G. D., Hudson, K. L. and Reece, K. S. ( 2004). Multiple polymorphic sites at the ITS and ATAN loci in cultured isolates of Perkinsus marinus. Journal of Eukaryotic Microbiology 51, 312–320.CrossRefGoogle Scholar
Burreson, E. M. and Ragone Calvo, L. M. ( 1996). Epizootiology of Perkinsus marinus disease of oysters in Chesapeake Bay, with emphasis on data since 1985. Journal of Shellfish Research 15, 17–34.Google Scholar
Bushek, D., Allen, S. K., Alcox, K. A., Gustafson, R. and Ford, S. E. ( 1994). Dose response of the eastern oyster, Crassostrea virginica, to cultured cells of Perkinsus marinus, the agent of Dermo disease. Journal of Shellfish Research 13, 313.Google Scholar
Bushek, D., Dungan, C. F. and Lewitus, A. J. ( 2002). Serological affinities of the oyster pathogen Perkinsus marinus (Apicomplexa) with some dinoflagellates (Dinophyceae). Journal of Eukaryotic Microbiology 49, 11–16.CrossRefGoogle Scholar
Bushek, D., Ford, S. E. and Chintala, M. M. ( 2002). Comparison of in vitro-cultured and wild type Perkinsus marinus. III. Fecal elimination and its role in transmission. Diseases of Aquatic Organisms 51, 217–225.Google Scholar
Bushek, D. and Howell, T. L. ( 2000). The effect of UV irradiation on Perkinsus marinus and its potential use to reduce transmission via shellfish effluents. Northeast Regional Aquaculture Center, University of Massachusetts, North Dartmouth, No. 00-008. 5 pp.
Carnegie, R. B., Ragone Calvo, L. M. and Burreson, E. M. ( 2004). Status of the Major Oyster Diseases in Virginia – 2003. A Summary of the Annual Monitoring Program. Virginia Institute of Marine Science Marine Resource Report.
Chintala, M. M., Bushek, D. and Ford, S. E. ( 2002). Comparison of in vitro-cultured and wild-type Perkinsus marinus. II. Dosing methods and host response. Diseases of Aquatic Organisms 51, 203–216.CrossRefGoogle Scholar
Chu, F. L. and La Peyre, J. F. ( 1993). Perkinsus marinus susceptibility and defense related activities in eastern oysters Crassostrea virginica: temperature effects. Diseases of Aquatic Organisms 16, 223–234.CrossRefGoogle Scholar
Chu, F. L. and Volety, A. K. ( 1997). Disease processes of the parasite Perkinsus marinus in eastern oyster Crassostrea virginica: minimum dose for infection initiation, and interaction of temperature, salinity and infective cell dose. Diseases of Aquatic Organisms 28, 61–68.CrossRefGoogle Scholar
Creswell, R. L., Vaughan, D. E. and Sturmer, L. N. ( 1991). Manual for Cultivation of the American Oyster, Crassostrea virginica, in Florida. Aquaculture Market Development Aid Program, Aquaculture Report Series, Florida department of Agriculture and Consumer Services, Tallahassee, Florida, 50 pp.
Dungan, C. F., Hamilton, R. M., Hudson, K. L., McCollough, C. B. and Reece, K. S. ( 2002). Two epizootic diseases in Chesapeake Bay commercial clams, Mya arenaria and Tagelus plebeius. Diseases of Aquatic Organisms 50, 67–78.CrossRefGoogle Scholar
Ford, S. E., Chintala, M. M. and Bushek, D. ( 2002). Comparison of in vitro-cultured and wild-type Perkinsus marinus. I. Pathogen virulence. Diseases of Aquatic Organisms 51, 187–201.CrossRefGoogle Scholar
Ford, S. E., Xu, Z. and Debrosse, G. ( 2001). Use of particle filtration and UV irradiation to prevent infection by Haplosporidium nelsoni MSX and Perkinsus marinus Dermo in hatchery-reared larval and juvenile oysters. Aquaculture 194, 37–49.CrossRefGoogle Scholar
Guy, R. A., Payment, P., Krull, U. J. and Horgen, P. A. ( 2003). Real-time PCR for quantification of Giardia and Cryptosporidium in environmental water samples and sewage. Applied and Environmental Microbiology 69, 5178–5185.CrossRefGoogle Scholar
Hadley, N. H., Bobo, M. Y., Richardson, D., Coen, L. D. and Bushek, D. ( 1996). Use of specific-pathogen-free (SPF) oysters to measure growth, mortality and onset of MSX and Dermo disease in South Carolina. Journal of Shellfish Research 15, 496.Google Scholar
Haskin, H. H. and Andrews, J. D. ( 1988). Uncertainties and speculations about the life cycle of the eastern oyster pathogen Haplosporidium nelsoni (MSX). American Fisheries Society Special Publications 18, 5–22.Google Scholar
Higgins, J. A., Fayer, R., Trout, J. M., Xiao, L., Lal, A. A., Kerby, S. and Jenkins, M. C. ( 2001). Real-time PCR for the detection of Cryptosporidium parvum. Journal of Microbiological Methods 47, 323–337.CrossRefGoogle Scholar
Higuchi, R., Fockler, C., Dollinger, G. and Watson, R. ( 1993). Kinetic PCR analysis: real-time monitoring of DNA amplification reactions. Biotechnology 11, 1026–1030.CrossRefGoogle Scholar
La Peyre, J. F., Faisal, M. and Burreson, E. M. ( 1993). In vitro propagation of the protozoan Perkinsus marinus, a pathogen of the eastern oyster, Crassostrea virginica. Journal of Eukaryotic Microbiology 40, 304–310.CrossRefGoogle Scholar
Mackin, J. G. ( 1956). Dermocystidium marinum and salinity. Proceedings of the National Shellfissheries Association 46, 116–133.Google Scholar
Mackin, J. G. ( 1962). Oyster diseases caused by Dermocystidium marinum and other microorganisms in Louisiana. Publications of the Institute of Marine Science, University of Texas 7, 132–229.Google Scholar
Monis, P. T., Andrews, R. H. and Saint, C. P. ( 2002). Molecular biology techniques in parasite ecology. International Journal for Parasitology 32, 551–562.CrossRefGoogle Scholar
Pecher, W. T., Robledo, J. A. F. and Vasta, G. R. ( 2004). Identification of a second rRNA gene unit in the Perkinsus andrewsi genome. Journal of Eukaryotic Microbiology 51, 234–245.CrossRefGoogle Scholar
Perkins, F. O. ( 1988). Structure of protistan parasites found in bivalve molluscs. American Fisheries Society Special Publication 18, 93–111.Google Scholar
Ragone Calvo, L. M. and Burreson, E. M. ( 1993). Effect of salinity on infection progression and pathogenicity of Perkinsus marinus in the eastern oyster, Crassostrea virginica (Gmelin). Journal of Shellfish Research 12, 1–7.Google Scholar
Ragone Calvo, L. M. and Burreson, E. M. ( 1994). Characterization of overwintering infections of Perkinsus marinus (Apicomplexa) in Chesapeake Bay oysters. Journal of Shellfish Research 13, 123–130.Google Scholar
Ragone Calvo, L. M. and Burreson, E. M. ( 2003). Status of the Major Oyster Diseases in Virginia – 2002. A Summary of the Annual Monitoring Program. Virginia Institute of Marine Science Marine Resource Report.
Ragone Calvo, L. M., Dungan, C. F., Roberson, B. S. and Burreson, E. M. ( 2003). Systematic evaluation of factors controlling Perkinsus marinus transmission dynamics in lower Chesapeake Bay. Diseases of Aquatic Organisms 56, 75–86.CrossRefGoogle Scholar
Ragone Calvo, L. M., Wetzel, R. L. and Burreson, E. M. ( 2000). Development and verification of a model for the population dynamics of the protistan parasite, Perkinsus marinus, within its host, the eastern oyster, Crassostrea virginica, in Chesapeake Bay. Journal of Shellfish Research 20, 231–241.Google Scholar
Ray, S. M. ( 1952). A culture technique for the diagnosis of infections with Dermocystidium marinum, Mackin, Owen and Collier, in Oysters. Science 166, 360–361.CrossRefGoogle Scholar
Ray, S. M. ( 1954 a). Studies on the occurrence of Dermocystidium marinum in young oysters. Convention Addresses National Shellfisheries Association 1953 44, 80–88.Google Scholar
Ray, S. M. ( 1954 b). Biological Studies of Dermocystidium marinum, a Fungus Parasite of Oysters. Rice Institute Pamphlet Special Issue, November.
Ray, S. M. and Mackin, J. G. ( 1954). Studies on transmission and pathogenicity of Dermocystidium marinum, I. Texas A&M Research Foundation Project, Technical Report 23, 1–11.Google Scholar
Reece, K. S., Siddall, M., Burreson, E. M. and Graves, J. E. ( 1997). Phylogenetic analysis of Perkinsus based on actin gene sequences. Journal of Parasitology 83, 417–423.CrossRefGoogle Scholar
Roberson, B. S. and Dungan, C. F. ( 1993). Flow cytometric enumeration and isolation of immunofluorescent Perkinsus marinus cells from estuarine waters. Journal of Shellfish Reasearch 12, 138.Google Scholar
Robledo, J. A. F., Gauthier, J. D., Coss, C. A., Wright, A. C. and Vasta, G. R. ( 2000). Species specificity and sensitivity of a PCR-based assay for Perkinsus marinus in the eastern oyster, Crassostrea virginica: a comparison with the fluid thioglycollate assay. Journal of Parasitology 84, 1237–1244.Google Scholar
Saldarriaga, J. F., McEwan, M. L., Fast, N. M., Taylor, F. J. and Keeling, P. J. ( 2003). Multiple protein phylogenies show that Oxyrrhis marina and Perkinsus marinus are early branches of the dinoflagellate lineage. International Journal of Systematic and Evolutionary Microbiology 53, 355–365.CrossRefGoogle Scholar
Scanlon, C. H. ( 1997). The potential for transmission of Perkinsus marinus by fecal matter from the eastern oyster, Crassostrea virginica. Master's thesis, Virginia Institute of Marine Science, Gloucester Point.
Siddall, M. E., Reece, K. S., Graves, J. E. and Burreson, E. M. ( 1997). “Total evidence” refutes the inclusion of Perkinsus species in the phylum Apicomplexa. Parasitology 115, 165–176.CrossRefGoogle Scholar
Soniat, T. M. and Gauthier, J. D. ( 1989). The prevalence and intensity of Perkinsus marinus from the mid northern Gulf of Mexico, with comments on the relationship of the oyster parasite to temperature and salinity. Tulane Studies in Zoology and Botany 27, 21–27.Google Scholar
Villalba, A., Reece, K. S., Ordas, C. M., Casas, S. M. and Figueras, A. ( 2004). Perkinsiosis in molluscs: a review. Aquatic Living Resources 17, 411–432.CrossRefGoogle Scholar
Volety, A. K. and Chu, F.-L. E. ( 1994). Comparison of infectivity and pathogenicity of meront (Trophozoite) and prezoosporangia stages of the oyster pathogen Perkinsus marinus in eastern oysters, Crassostrea virginica (Gmelin, 1791). Journal of Shellfish Research 13, 521–527.Google Scholar
Wilson, I. G. ( 1997). Inhibition and facilitation of nucleic acid amplification. Applied and Environmental Microbiology 63, 3741–3751.Google Scholar
Wittwer, C. T., Ririe, K. M., Andrew, R. V., David, D. A., Gundry, R. A. and Balis, U. J. ( 1997). The LightCycler™: a microvolume multisample fluorimeter with rapid temperature control. Biotechniques 22, 176–181.Google Scholar