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Quantitative studies on the kinetics of establishment and expulsion of intestinal nematode populations in susceptible and immune hosts. Nippostrongylus brasiliensis in the rat

Published online by Cambridge University Press:  06 April 2009

Ellen E. E. Jarrett
Affiliation:
Wellcome Laboratories for Experimental Parasitology, University of Glasgow, Scotland
W. F. H. Jarrett
Affiliation:
Wellcome Laboratories for Experimental Parasitology, University of Glasgow, Scotland
G. M. Urquhart
Affiliation:
Wellcome Laboratories for Experimental Parasitology, University of Glasgow, Scotland

Extract

This paper describes the quantitative aspects of establishment and expulsion of Nippostrongylus brasiliensis in the normal and immune rat. It is proposed that this helminth infection may be described in terms of four phases. These are: (1) loss phase 1 (LP1). A proportion of the infecting dose of larvae is immobilized or destroyed before or during the course of migration via the lungs to the intestine. (2) The plateau phase. The worm number remains static in the predilection site. (3) Loss phase 2 (LP2). This is analogous to the self-cure reaction and during this worms are expelled from the host by an immunological mechanism. The onset of the expulsion is sudden and the process is exponential. It continues at a steady rate until a threshold is reached. (4) Threshold phase. A small residual population of worms is not expelled and survives for a fairly prolonged period.

The pattern is maintained in immune animals undergoing second or third infections but there are quantitative differences. With increasing number of infections LP1 becomes greater, the plateau phase is shortened and the expulsion rate of LP 2 increases. It is suggested that the rate coefficient of this phase is an accurate measure of the immune status of the animal. The threshold phase is of a similar order in first, second and third infections. These results are discussed with regard to their significance as a measure of the immune status of the host, their implication regarding the mechanism of helminth expulsion and their relevance to other helminth infections.

The authors wish to thank the Agricultural Research Council and Allen and Hanburys Ltd. for the financial support which made this work possible. During this period Ellen E. Jarrett held a Horserace Betting Levy Board Scholarship.

Type
Research Article
Copyright
Copyright © Cambridge University Press 1968

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References

REFERENCES

Africa, C. M. (1931). Studies on the host relations of Nippostrongylus muris. J. Parasit. 18, 113.CrossRefGoogle Scholar
Armour, J. (1967). Ph.D. Thesis, University of Glasgow.Google Scholar
Armour, J., Jarrett, W. F. H. & Jennings, F. W. (1966). Experimental Ostertagia circumcincta infections in sheep: development and pathogenesis of a single infection. Am. J. vet. Res. 27, 1267–78.Google Scholar
Bakarat, M. R. (1951). A new procedure for the cultivation of the nematode parasites. J. Egypt. Med. Ass. 34, 323–6.Google Scholar
Ciordia, H. & Bizzell, W. E. (1960). Some effects of X-rays on the infective larvae of the cattle nematode Trichostrongylus axei. Expl Parasit. 9, 37.CrossRefGoogle ScholarPubMed
Goldstein, A. (1964). Biostatistics. London: Macmillan.Google Scholar
Gordon, H. McL. & Whitlock, H. V. (1939). A new technique for counting nematode eggs in sheep faeces. J. Coun. sci. ind. Res. Aust. 12, 50–2.Google Scholar
Jarrett, W. F. H., Jarrett, Ellen E., Miller, H. R. P. & Urquhart, G. M. (1967). Quantitative studies on the mechanism of self cure in Nippostrongylus brasiliensis infections. Proc. World Ass. Advancement Vet. Parasitol., Lyon, 1967. 3rd Int. Conf. In the Press.Google Scholar
Jennings, F. W., Mulligan, W. & Urquhart, G. M. (1963). Variables in X-ray ‘inactivation’ of Nippostrongylus brasiliensis larvae. Expl Parasit. 13, 367–73.CrossRefGoogle ScholarPubMed
Kassai, T., Fitzpatrick, B. & Mulligan, W. (1966). Variables in the radiation-attenuation of helminth larvae: the effect of differences in quality of radiation. Parasitology 56, 651–6.CrossRefGoogle ScholarPubMed
Mulligan, W., Urquhart, G. M., Jennings, F. W. & Neilson, J. T. M. (1965). Immunological studies on Nippostrongylus brasiliensis infection in the rat: the ‘self-cure’ phenomenon. Expl Parasit. 16, 341–7.CrossRefGoogle ScholarPubMed
Sarles, M. P. & Taliaferro, W. H. (1936). The local points of defense and passive transfer of acquired immunity to Nippostrongylus muris in rats. J. infect. Dis. 59, 207–20.CrossRefGoogle Scholar
Snedecor, G. W. (1966). Statistical Methods, 6th edition. Ames, Iowa, U.S.A.: The Iowa State University Press.Google Scholar
Taliaferro, W. H. & Sarles, M. P. (1939). The cellular reactions in the skin lungs and intestine of normal and immune rats after infection with Nippostrongylus muris. J. infect. Dis. 64, 157–92.CrossRefGoogle Scholar
Thorson, R. E. (1954). Effect of immune serum from rats on infective larvae of Nippostrongylus muris in the rat. Expl Parasit. 3, 915.CrossRefGoogle Scholar
Wilson, R. J. M. (1966). y 1-Antibodies in guinea pigs infected with the cattle lungworm. Immunology 11, 199209.Google ScholarPubMed