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Proteomic analysis of Strongyloides stercoralis L3 larvae

Published online by Cambridge University Press:  14 April 2010

A. MARCILLA ,
J. SOTILLO ,
A. PÉREZ-GARCIA ,
R. IGUAL-ADELL ,
M. LUZ VALERO ,
M. M. SÁNCHEZ-PINO ,
D. BERNAL ,
C. MUÑOZ-ANTOLÍ ,
M. TRELIS  and
R. TOLEDO
...Show all authors
A. MARCILLA*
Affiliation:
Área de Parasitología, Departament de Biologia Cel.lular i Parasitologia, Universitat de València, Burjassot, Valencia, Spain
J. SOTILLO
Affiliation:
Área de Parasitología, Departament de Biologia Cel.lular i Parasitologia, Universitat de València, Burjassot, Valencia, Spain
A. PÉREZ-GARCIA
Affiliation:
Área de Parasitología, Departament de Biologia Cel.lular i Parasitologia, Universitat de València, Burjassot, Valencia, Spain
R. IGUAL-ADELL
Affiliation:
Sección de Microbiología, Hospital General de Castellón, Castellón, Spain
M. LUZ VALERO
Affiliation:
Servicio de Proteómica, Centro de Investigación “Principe Felipe”, Valencia, Spain
M. M. SÁNCHEZ-PINO
Affiliation:
Servicio de Proteómica, Centro de Investigación “Principe Felipe”, Valencia, Spain
D. BERNAL
Affiliation:
Departament de Bioquímica i Biologia Molecular, Universitat de València, Burjassot, Valencia, Spain
C. MUÑOZ-ANTOLÍ
Affiliation:
Área de Parasitología, Departament de Biologia Cel.lular i Parasitologia, Universitat de València, Burjassot, Valencia, Spain
M. TRELIS
Affiliation:
Área de Parasitología, Departament de Biologia Cel.lular i Parasitologia, Universitat de València, Burjassot, Valencia, Spain
R. TOLEDO
Affiliation:
Área de Parasitología, Departament de Biologia Cel.lular i Parasitologia, Universitat de València, Burjassot, Valencia, Spain
J. G. ESTEBAN
Affiliation:
Área de Parasitología, Departament de Biologia Cel.lular i Parasitologia, Universitat de València, Burjassot, Valencia, Spain
*
*Corresponding author: Departament de Biologia Cel.lular i Parasitologia, Facultat de Farmàcia, Universitat de València, Av. V.A. Estellés, s/n, 46100 Burjassot (Valencia), Spain. Tel: +34 963544491. Fax: +34 963544769. E-mail: [email protected]
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Summary

Strongyloidiasis can be perpetuated by autoinfection with the filariform larvae L3, causing asymptomatic chronic infections and creating a population of carriers, affecting not only developing countries. So far, very little is known about the proteins that interact with the human host, and few proteins from the infective Strongyloides stercoralis L3 have been characterized. Here, we report results obtained from a proteomic analysis of the proteins from S. stercoralis L3 larvae obtained from patients. Since the genome of S. stercoralis is not yet available, we used proteomic analysis to identify 26 different proteins, 13 of them released by short digestion with trypsin, which could represent surface-associated proteins. The present work extends our knowledge of host-parasite interactions by identifying proteins that could be of interest in the development of diagnostic tools, vaccines, or treatments for a neglected disease like strongyloidiasis.

Keywords

Strongyloides stercoralis larvaehost-parasite interactionproteomics
Type
Research Article
Information
Parasitology , Volume 137 , Issue 10 , September 2010 , pp. 1577 - 1583
Copyright
Copyright © Cambridge University Press 2010

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References

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