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Proteomic analysis of a 120 kDa protein complex in cyst fluid of Taenia solium metacestode and preliminary evaluation of its value for the serodiagnosis of neurocysticercosis

Published online by Cambridge University Press:  15 August 2005

E.-G. LEE ,
Y.-A. BAE ,
Y.-T. JEONG ,
J.-Y. CHUNG ,
E.-Y. JE ,
S.-H. KIM ,
B.-K. NA ,
J.-W. JU ,
T.-S. KIM  and
L. MA
...Show all authors
E.-G. LEE
Affiliation:
Department of Molecular Parasitology and Center for Molecular Medicine, Samsung Biomedical Research Institute and Sungkyunkwan University School of Medicine, Suwon 440-746, Korea
Y.-A. BAE
Affiliation:
Department of Molecular Parasitology and Center for Molecular Medicine, Samsung Biomedical Research Institute and Sungkyunkwan University School of Medicine, Suwon 440-746, Korea
Y.-T. JEONG
Affiliation:
Department of Molecular Parasitology and Center for Molecular Medicine, Samsung Biomedical Research Institute and Sungkyunkwan University School of Medicine, Suwon 440-746, Korea
J.-Y. CHUNG
Affiliation:
PharmacoGenomics Research Center, Inje University, Busan 614-735, Korea
E.-Y. JE
Affiliation:
Department of Molecular Parasitology and Center for Molecular Medicine, Samsung Biomedical Research Institute and Sungkyunkwan University School of Medicine, Suwon 440-746, Korea
S.-H. KIM
Affiliation:
Department of Molecular Parasitology and Center for Molecular Medicine, Samsung Biomedical Research Institute and Sungkyunkwan University School of Medicine, Suwon 440-746, Korea
B.-K. NA
Affiliation:
Department of Molecular Parasitology and Center for Molecular Medicine, Samsung Biomedical Research Institute and Sungkyunkwan University School of Medicine, Suwon 440-746, Korea
J.-W. JU
Affiliation:
Department of Tropical and Endemic Parasitic Diseases, National Institute of Health, Seoul 122-701, Korea
T.-S. KIM
Affiliation:
Department of Tropical and Endemic Parasitic Diseases, National Institute of Health, Seoul 122-701, Korea
L. MA
Affiliation:
Infectious Diseases Section, Department of Medicine, Louisiana State University Health Sciences Center, New Orleans, LA 70112, USA
S.-Y. CHO
Affiliation:
Department of Molecular Parasitology and Center for Molecular Medicine, Samsung Biomedical Research Institute and Sungkyunkwan University School of Medicine, Suwon 440-746, Korea
Y. KONG
Affiliation:
Department of Molecular Parasitology and Center for Molecular Medicine, Samsung Biomedical Research Institute and Sungkyunkwan University School of Medicine, Suwon 440-746, Korea
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Abstract

Cyst fluid (CF) of Taenia solium metacestode (TsM) is an important source of serodiagnostic antigens. We have investigated the molecular characteristics of the 120 kDa protein complex in TsM CF purified by fast performance liquid chromatography. The structure of the purified protein was characterized by a variety of proteomic analyses. The protein was found to consist of 2 major components of 42–46 and 22–28 kDa, and shared 3 subunits of 14, 16 and 18 kDa. The 42–46 kDa component was determined to contain 3 additional subunits of 22, 28 and 38 kDa. These 6 subunits were shown to originate from either the 14 or 18 kDa precursor. We assessed the antibody reactivity of the native protein, its individual subunits and the recombinant 14 and 18 kDa proteins, and found that the 120 kDa protein, particularly 14 and 18 kDa subunits revealed high reliability for differentiation of active and mixed stage NC from chronic NC. The subunits of the 120 kDa protein complex identified herein represent some of the low-molecular weight glycoproteins which have been described in several previous studies. Recognizing and understanding the structural and immunological relationship of these proteins will facilitate the development of new serodiagnostic assays.

Keywords

Taenia solium neurocysticercosis120 kDa glycoproteinMALDI-TOF MS14 kDa18 kDalow-molecular weight glycoproteinserodiagnostic antigen
Type
Research Article
Information
Parasitology , Volume 131 , Issue 6 , December 2005 , pp. 867 - 879
Copyright
© 2005 Cambridge University Press

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References

REFERENCES

Baranwal, A. K., Singhi, P. D., Khandelwal, N. and Singhi, S. C. ( 1998). Albendazole therapy in children with focal seizures and single small enhancing computerized tomographic lesions: a randomized, placebo-controlled, double blind trial. Pediatric Infectious Diseases Journal 17, 696–700.CrossRefGoogle Scholar
Bern, C., Garcia, H. H., Evans, C., Gonzalez, A. E., Verastegui, M., Tsang, V. C. and Gilman, R. H. ( 1999). Magnitude of the disease burden from neurocysticercosis in a developing country. Clinical Infectious Diseases 29, 1203–1209.CrossRefGoogle Scholar
Blum, H., Beier, H. and Grass, H. J. ( 1987). Improved silver staining of plant proteins, RNA and DNA in polyacrylamide gels. Electrophoresis 8, 93–99.CrossRefGoogle Scholar
Carpio, A., Escobar, A. and Hauser, W. ( 1998). Cysticercosis and epilepsy: a critical review. Epilepsia 39, 1025–1040.CrossRefGoogle Scholar
Cho, S. Y., Kim, S. I., Kang, S. Y., Choi, D. Y., Suk, J. S., Choi, K. S., Ha, Y. S., Chung, C. S. and Myung, H. J. ( 1986). Evaluation of enzyme-linked immunosorbent assay in serological diagnosis of human neurocysticercosis using paired samples of serum and cerebrospinal fluid. Korean Journal of Parasitology 24, 25–41.CrossRefGoogle Scholar
Cho, S. Y., Kim, S. I., Kang, S. Y. and Kong, Y. ( 1988). Biochemical properties of a purified protein in cystic fluid of Taenia solium metacestodes. Korean Journal of Parasitology 26, 87–94.CrossRefGoogle Scholar
Chung, J. Y., Bahk, Y. Y., Huh, S., Kang, S. Y., Kong, Y. and Cho, S. Y. ( 1999). A recombinant 10 kDa protein of Taenia solium metacestodes specific to active neurocysticercosis. Journal of Infectious Diseases 180, 1307–1315.CrossRefGoogle Scholar
Del Brutto, O. H., Rajshekhar, V., White, A. C. Jr., Tsang, V. C., Nash, T. E., Takayanagui, O. M., Schantz, P. M., Evans, C. A., Flisser, A., Correa, D., Botero, D., Allan, J. C., Sarti, E., Gonzalez, A. E., Gilman, R. H. and Garcia, H. H. ( 2001). Proposed diagnostic criteria for neurocysticercosis. Neurology 57, 177–183.CrossRefGoogle Scholar
Del Brutto, O. H., Sotelo, J. and Roman, G. C. ( 1998). Neurocysticercosis: a Clinical Handbook. 1st Edn. Swets and Zeitlinger Publishers, Lisse.
Eom, K. S., Jeon, H. K., Kong, Y., Hwang, U. W., Yang, Y., Li, X., Xu, L., Feng, Z., Pawlowski, Z. S. and Rim, H. J. ( 2002). Identification of Taenia asiatica in China: molecular, morphological, and epidemiological analysis of a Luzhai isolate. Journal of Parasitology 88, 758–764.CrossRefGoogle Scholar
Escobar, A. ( 1983). The pathology of neurocysticercosis. In Cysticercosis of the Central Nervous System ( ed. Palacio, E., Rodriguez-Cabajal, J. and Taveras, J. M.), pp. 27–54. Thomas, Springfield, USA.
Garcia, H. H., Evans, C. A., Nash, T. E., Takayanagui, O. M., White, A. C. Jr., Botero, D., Rajshekhar, V., Tsang, V. C., Schantz, P. M., Allan, J. C., Flisser, A., Correa, D., Sarti, E., Friedland, J. S., Martinez, S. M., Gonzalez, A. E., Gilman, R. H. and Del Brutto, O. H. ( 2002). Current consensus guidelines for treatment of neurocysticercosis. Clinical Microbiology Review 15, 747–756.CrossRefGoogle Scholar
Garcia, H. H., Gonzalez, A. E., Gilman, R. H. and Cysticerosis Working Group in Peru. ( 2003). Diagnosis, treatment and control of Taenia solium cysticercosis. Current Opinion in Infectious Diseases 16, 411–419.CrossRefGoogle Scholar
Garcia, H. H., Pretell, E. J., Gilman, R. H., Martinez, S. M., Moulton, L. H., Del Brutto, O. H., Herrera, G., Evans, C. A., Gonzalez, A. E. and Cysticercosis Working Group in Peru. ( 2004). A trial of antiparasitic treatment to reduce the rate of seizures due to cerebral cysticercosis. New England Journal of Medicine 350, 249–258.CrossRefGoogle Scholar
Görg, A., Obermaier, C., Boguth, G., Harder, A., Scheibe, B., Wildgruber, R. and Weiss, W. ( 2000). The current state of two-dimensional electrophoresis with immobilized pH gradients. Electrophoresis 21, 1037–1053.3.0.CO;2-V>CrossRefGoogle Scholar
Greene, R. M., Hancock, K., Wilkins, P. P. and Tsang, V. C. ( 2000). Taenia solium: molecular cloning and serologic evaluation of 14- and 18-kDa related, diagnostic antigens. Journal of Parasitology 86, 1001–1007.CrossRefGoogle Scholar
Greene, R. M., Wilkins, P. P. and Tsang, V. C. ( 1999). Diagnostic glycoproteins of Taenia solium cysts share homologous 14- and 18-kDa subunits. Molecular and Biochemical Parasitology 99, 257–261.CrossRefGoogle Scholar
Hancock, K., Khan, A., Williams, F. B., Yushak, M. L., Pattabhi, S., Noh, J. and Tsang, V. C. ( 2003). Characterization of the 8-kilodalton antigens of Taenia solium metacestodes and evaluation of their use in an enzyme-linked immunosorbent assay for serodiagnosis. Journal of Clinical Microbiology 41, 2577–2586.CrossRefGoogle Scholar
Haslam, S. M., Restrepo, B. I., Obregon-Henao, A., Teale, J. M., Morris, H. R. and Dell, A. ( 2003). Structural characterization of the N-linked glycans from Taenia solium metacestodes. Molecular and Biochemical Parasitology 126, 103–107.CrossRefGoogle Scholar
Ito, A., Plancarte, A., Ma, L., Kong, Y., Flisser, A., Cho, S. Y., Liu, Y. H., Kamhawi, S., Lightowlers, M. W. and Schantz, P. M. ( 1998). Novel antigens for neurocysticercosis: simple method for preparation and evaluation for serodiagnosis. American Journal of Tropical Medicine and Hygiene 59, 291–294.CrossRefGoogle Scholar
Kim, S. I., Kang, S. Y., Cho, S. Y., Hwang, E. S. and Cha, C. Y. ( 1986). Purification of cystic fluid antigen of Taenia solium metacestodes by affinity chromatography using monoclonal antibody and its antigenic characterization. Korean Journal of Parasitology 24, 145–158.CrossRefGoogle Scholar
Ko, R. C. and Ng, T. F. ( 1998). Purification of larval Taenia solium antigens by isoelectric focusing. Veterinary Parasitology 74, 191–202.CrossRefGoogle Scholar
Landry, F., Lombardo, C. R. and Smith, J. W. ( 2000). A method for application of samples to matrix-assisted laser desorption ionization time-of-flight targets that enhances peptide detection. Analytical Biochemistry 279, 1–8.CrossRefGoogle Scholar
Obregon-Henao, A., Gil, D. L., Gomez, D. I., Sanzon, F., Teale, J. M. and Restrepo, B. I. ( 2001). The role of N-linked carbohydrates in the antigenicity of Taenia solium metacestode glycoproteins of 12, 16 and 18 kD. Molecular and Biochemical Parasitology 114, 209–215.CrossRefGoogle Scholar
Page, R. D. ( 1996). Tree view: an application to display phylogenetic trees on personal computers. Computerized Applied Biosciences 12, 357–358.CrossRefGoogle Scholar
Palacio, L. G., Jimenez, I., Garcia, H. H., Jimenez, M. E., Sanchez, J. L., Noh, J., Ahn, L., Mora, O., Giraldo, M., Tsang, V. C. The Neuroepidemiological Research Group of Antioquia. ( 1998). Neurocysticercosis in persons with epilepsy in Medellin, Colombia. Epilepsia 39, 1334–1339.CrossRefGoogle Scholar
Phiri, I. K., Ngowi, H., Afonso, S., Matenga, E., Boa, M., Mukaratirwa, S., Githigia, S., Saimo, M., Sikasunge, C., Maingi, N., Lubega, G. W., Kassuku, A., Michael, L., Siziya, S., Krecek, R. C., Noormahomed, E., Vilhena, M., Dorny, P. and Willingham, A. L. 3rd. ( 2003). The emergence of Taenia solium cysticercosis in Eastern and Southern Africa as a serious agricultural problem and public health risk. Acta Tropica 87, 13–23.CrossRefGoogle Scholar
Restrepo, B. I., Obregon-Henao, A., Mesa, M., Gil, D. L., Ortiz, B. L., Mejia, J. S., Villota, G. E., Sanzon, F. and Teale, J. M. ( 2000). Characterisation of the carbohydrate components of Taenia solium metacestode glycoprotein antigens. International Journal for Parasitology 30, 689–696.CrossRefGoogle Scholar
Roman, G., Sotelo, J., Del Brutto, O. H., Flisser, A., Dumas, M., Wadia, N., Botero, D., Cruz, M., Garcia, H. H., De Bittencourt, P. R., Trelles, L., Arriagada, C., Lorenzana, P., Nash, T. E. and Spina-Franca, A. ( 2000). A proposal to declare neurocysticercosis an international reportable disease. Bulletin of the World Health Organization 78, 399–406.Google Scholar
Sako, Y., Nakao, M., Ikejima, T., Piao, X. Z., Nakaya, K. and Ito, A. ( 2000). Molecular characterization and diagnostic value of Taenia solium low-molecular-weight antigen genes. Journal of Clinical Microbiology 38, 4439–4444.Google Scholar
Tsang, V. C., Brand, J. A. and Boyer, A. E. ( 1989). An enzyme-linked immunoelectrotransfer blot assay and glycoprotein antigens for diagnosing human cysticercosis (Taenia solium). Journal of Infectious Diseases 159, 50–59.CrossRefGoogle Scholar
Vilhena, M., Santos, M. and Torgal, J. ( 1999). Seroprevalence of human cysticercosis in Maputo, Mozambique. American Journal of Tropical Medicine and Hygiene 61, 59–62.CrossRefGoogle Scholar
Villota, G. E., Gomez, D. I., Volcy, M., Franco, A. F., Cardona, E. A., Isaza, R., Sanzon, F., Teale, J. M. and Restrepo, B. I. ( 2003). Similar diagnostic performance for neurocysticercosis of three glycoprotein preparations from Taenia solium metacestodes. American Journal of Tropical Medicine and Hygiene 68, 276–280.Google Scholar
White, A. C. Jr., Robinson, P. and Kuhn, R. ( 1997). Taenia solium cysticercosis: host-parasite interactions and immune response. Chemical Immunology 66, 209–230.CrossRefGoogle Scholar
White, A. C. Jr. ( 2000). Neurocysticercosis: updates on epidemiology, pathogenesis, diagnosis, and management. Annual Review of Medicine 51, 187–206.CrossRefGoogle Scholar
Yang, H. J., Chung, J. Y., Yun, D. H., Kong, Y., Ito, A., Ma, L., Liu, Y. H., Lee, S. C., Kang, S. Y. and Cho, S. Y. ( 1998). Immunoblot analysis of a 10 kDa antigen in cyst fluid of Taenia solium metacestodes. Parasite Immunology 20, 483–488.CrossRefGoogle Scholar