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Protein deficiency alters impact of intestinal nematode infection on intestinal, visceral and lymphoid organ histopathology in lactating mice

Published online by Cambridge University Press:  05 February 2014

LISA M. STARR ,
MAURICE R. ODIERE ,
KRISTINE G. KOSKI  and
MARILYN E. SCOTT
LISA M. STARR*
Affiliation:
Institute of Parasitology, McGill University (Macdonald Campus), Ste-Anne-de-Bellevue, Quebec, H9X 3V9, Canada
MAURICE R. ODIERE
Affiliation:
Institute of Parasitology, McGill University (Macdonald Campus), Ste-Anne-de-Bellevue, Quebec, H9X 3V9, Canada
KRISTINE G. KOSKI
Affiliation:
School of Dietetics and Human Nutrition, McGill University (Macdonald Campus), Ste-Anne-de-Bellevue, Quebec, H9X 3V9, Canada
MARILYN E. SCOTT
Affiliation:
Institute of Parasitology, McGill University (Macdonald Campus), Ste-Anne-de-Bellevue, Quebec, H9X 3V9, Canada
*
* Corresponding author: Institute of Parasitology, McGill University (Macdonald Campus), Ste-Anne-de-Bellevue, Quebec, H9X 3V9, Canada. E-mail: [email protected]
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Summary

Protein deficiency impairs local and systemic immune responses to Heligmosomoides bakeri infection but little is known about their individual and interactive impacts on tissue architecture of maternal lymphoid (thymus, spleen) and visceral (small intestine, kidney, liver, pancreas) organs during the demanding period of lactation. Using a 2×2 factorial design, pregnant CD1 mice were fed a 24% protein sufficient (PS) or a 6% protein deficient (PD) isoenergetic diet beginning on day 14 of pregnancy and were infected with 100 H. bakeri larvae four times or exposed to four sham infections. On day 20 of lactation, maternal organs were examined histologically and serum analytes were assayed as indicators of organ function. The absence of villus atrophy in response to infection was associated with increased crypt depth and infiltration of mast cells and eosinophils but only in lactating dams fed adequate protein. Infection-induced lobular liver inflammation was reduced in PD dams, however, abnormalities in the kidney caused by protein deficiency were absent in infected dams. Bilirubin and creatinine were highest in PD infected mice. Infection-induced splenomegaly was not due to an increase in the lymphoid compartment of the spleen. During lactation, infection and protein deficiency have interactive effects on extra-intestinal pathologies.

Keywords

Heligmosomoides bakeriprotein deficiencyextra-intestinal histopathologylactationnematodetrickle infectionliverspleenkidneyduodenum
Type
Research Article
Information
Parasitology , Volume 141 , Issue 6 , May 2014 , pp. 801 - 813
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Copyright
Copyright © Cambridge University Press 2014 

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