Hostname: page-component-586b7cd67f-vdxz6 Total loading time: 0 Render date: 2024-11-28T07:18:41.308Z Has data issue: false hasContentIssue false
  • English
  • Français

Propagation of Nosema eurytremae (Microsporida: Nosematidae) from trematode larvae, in abnormal hosts and in tissue culture

Published online by Cambridge University Press:  06 April 2009

G. C. Higby ,
Elizabeth U. Canning ,
Barbara M. Pilley  and
P. J. Bush
G. C. Higby
Affiliation:
Department of Zoology and Applied Entomology, Imperial College, London
Elizabeth U. Canning
Affiliation:
Department of Zoology and Applied Entomology, Imperial College, London
Barbara M. Pilley
Affiliation:
Department of Zoology and Applied Entomology, Imperial College, London
P. J. Bush
Affiliation:
Department of Zoology and Applied Entomology, Imperial College, London
Get access Rights & Permissions [Opens in a new window]

Summary

Propagation of Nosema eurytremae, a microsporidian pathogen of trematode larvae, was investigated by inoculation of spores into the haemocoele of insects and by growth in tissue cultures. Locusts and the larvae of three lepidopteran species were good hosts but cockroaches were not. Low replication was obtained in one lepidopteran species after per os infection. Antibiotics controlled bacterial growth in suspensions of microsporidian spores but fungi were unaffected by all antibiotics tested, except at concentrations detrimental to the microsporidia. All stages of the microsporidium developed in cell lines of Xenopus laevis and Aedes pseudoscutellaris when spores were induced to hatch in contact with cell monolayers: the Aedes culture was contaminated by yeasts. Repeated washing of the Xenopus cells with fresh medium, after the sporoplasms of N. eurytremae had penetrated the cells, removed yeast contaminants and sterile cultures were obtained. Replication during 4 passages over 53 days was only 100 to 200-fold compared with the original inoculum but spores harvested from the cultures were infective to a fresh culture and to Pieris brassicae by inoculation into the haemocoele.

Type
Research Article
Information
Parasitology , Volume 78 , Issue 2 , April 1979 , pp. 155 - 170
Copyright
Copyright © Cambridge University Press 1979

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

Bayne, C. J., Owczarzak, A. & Noonan, W. E. (1975). In vitro cultivation of cells and a microsporidian parasite of Biomphalaria glabrata (Pulmonata: Basommatophora). Annals of the New York Academy of Science 266, 513–27.CrossRefGoogle Scholar
Canning, E. U. (1975). The microsporidian parasites of Platyhelminthes: their morphology, development, transmission and pathogenicity. Commonwealth Institute of Helminthology. Miscellaneous Publications No. 2, pp. 1–32.Google Scholar
Colley, F. C., Lie, K. J. & Cheong, W. H. (1971). Experimental infection of mosquito larvae with Perezia helminthorum (Microsporidia: Nosematidae). Southeast Asian Journal of Tropical Medicine and Public Health 2, 411.Google Scholar
Colley, F. C., Lie, K. J., Zaman, V. & Canning, E. U. (1975). Light and electron-microscopic study of Nosema eurytremae Canning 1972. Journal of Invertebrate Pathology 26, 11–20.CrossRefGoogle Scholar
Cox, J. C. & Pye, D. (1975). Serodiagnosis of nosematosis by immunofluorescence using cell-culture-grown organisms. Laboratory Animals 9, 297–304.CrossRefGoogle ScholarPubMed
David, W. A. L. & Gardiner, B. O. C. (1965). Rearing Pieris brassicae L. larvae on a semi-synthetic diet. Nature, London 207, 882–3.CrossRefGoogle Scholar
Fowler, J. L. & Reeves, E. L. (1975). In vivo propagation of a microsporidian pathogenic to insects. Journal of Invertebrate Pathology 25, 349–53.CrossRefGoogle Scholar
Gupta, K. S. (1964). Cultivation of Nosema mesnili Paillot (Microsporidia) in vitro. Current Science, India 33, 407–8.Google Scholar
Higby, G. C., Canning, E. U. & Pilley, B. M. (1978). Cultivation of a microsporidium in vertebrate and invertebrate cell lines. Journal of Protozoology (in the Press).Google Scholar
Ishihara, R. (1967). Stimuli causing extrusion of polar filaments of Glugea fumiferanae spores. Canadian Journal of Microbiology 13, 1321–32.CrossRefGoogle ScholarPubMed
Ishihara, R. (1968). Growth of Nosema bombycis in primary cell cultures of mammalian and chicken embryos. Journal of Invertebrate Pathology 11, 328–9.CrossRefGoogle Scholar
Ishihara, R. (1969). The life cycle of Nosema bornbycis as revealed in tissue culture cells of Bombyx mori. Journal of Invertebrate Pathology 14, 316–20.CrossRefGoogle ScholarPubMed
Ishihara, R. & Sohi, S. S. (1966). Infection of ovarian tissue culture of Bombyx mori by Nosema bombycis spores. Journal of Invertebrate Pathology 8, 538–40.CrossRefGoogle Scholar
Kurtti, T. J. & Brooks, M. A. (1971). Growth of a microsporidian parasite in cultured cells of tent caterpillars (Malacosoma). Current Topics in Microbiology and Immunology 55, 204–8.Google ScholarPubMed
Kurtti, T. J. & Brooks, M. A. (1977). The rate of development of a microsporidian in moth cell culture. Journal of Invertebrate Pathology 29, 126–32.CrossRefGoogle Scholar
Lie, K. J. & Colley, F. C. (1971). Stages in the life cycle of Perezia helminthorum (Microsporida, Nosematidae) from the rediae of Echinostoma malayanum. Southeast Asian Journal of Tropical Medicine and Public Health 2, 410–11.Google Scholar
Lie, K. J. & Nasemary, M. (1973). Transmission of Nosema eurytremae (Microsporida: Nosematidae) to various trematode larvae. Zeitschrift für Parasitenkunde 41, 109–17.Google Scholar
Margileth, A. M., Strano, A. J., Chandra, R., Neafie, R., Blum, M. & McCully, R. M. (1973). Disseminated nosematosis in an immunologically compromised infant. Archives of Pathology 95, 145–50.Google Scholar
Oshima, K. (1937). On the function of the polar filament of Nosema bombycis. Parasitology 29, 220–4.CrossRefGoogle Scholar
Oshima, K. (1964). Stimulative or inhibitive substance to evaginate the filament Nosema bombycis Nägeli. I. The case of artificial buffer solution. Japan Journal of Zoology 14, 209–29.Google Scholar
Seibold, H. R. & Fussell, E. N. (1973). Intestinal microsporidiosis in Callicebus moloch. Laboratory Animal Science 23, 115–18.Google ScholarPubMed
Sohi, S. S. & Wilson, G. G. (1976). Persistent infection of Malacosoma disstria (Lepidoptera, Lasicampidae) cell cultures with Nosema (Glugea) disstriae (Microsporida: Nosematidae). Canadian Journal of Zoology 54, 336–42.CrossRefGoogle Scholar
Trager, W. (1937). The hatching of spores of Nosema bombycis Nägeli and the partial development of the organism in tissue cultures. Journal of Parasitology 23, 220–7.CrossRefGoogle Scholar
Undeen, A. H. (1975). Growth of Nosema algerae in pig kidney cell cultures. Journal of Protozoology 22, 107–10.CrossRefGoogle ScholarPubMed
Undeen, A. H. & Alger, N. E. (1976). Nosema algerae: infection of the white mouse by a mosquito parasite. Experimental Parasitology 40, 86–8.CrossRefGoogle ScholarPubMed
Undeen, A. H. & Maddox, J. V. (1973). The infection of non-mosquito hosts by injection with spores of the microsporidian Nosema algerae. Journal of Invertebrate Pathology 22, 258–65.CrossRefGoogle Scholar
Weidner, E. (1972). Ultrastructural study of microsporidian invasion into cells. Zeitschrift für Parasitenkunde 40, 227–42.CrossRefGoogle ScholarPubMed
Wigglesworth, V. B. (1972). The Principles of Insect Physiology, 7th ed. London: Chapman and Hall.CrossRefGoogle Scholar