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Preferential infection of dividing cells by Cryptosporidium parvum

Published online by Cambridge University Press:  20 April 2006

G. WIDMER ,
Y. L. YANG ,
R. BONILLA ,
S. TANRIVERDI  and
K.M. CIOCIOLA
G. WIDMER
Affiliation:
Tufts Cummings School of Veterinary Medicine, Division of Infectious Diseases, 200 Westboro Road, North Grafton, MA 01536, USA
Y. L. YANG
Affiliation:
Tufts Cummings School of Veterinary Medicine, Division of Infectious Diseases, 200 Westboro Road, North Grafton, MA 01536, USA
R. BONILLA
Affiliation:
Tufts Cummings School of Veterinary Medicine, Division of Infectious Diseases, 200 Westboro Road, North Grafton, MA 01536, USA
S. TANRIVERDI
Affiliation:
Tufts Cummings School of Veterinary Medicine, Division of Infectious Diseases, 200 Westboro Road, North Grafton, MA 01536, USA
K.M. CIOCIOLA
Affiliation:
Tufts Cummings School of Veterinary Medicine, Division of Infectious Diseases, 200 Westboro Road, North Grafton, MA 01536, USA Present address: Genzyme Corporation, Pharmacogenetics Department, Box 9322 Framingham, MA 01701-9322, USA.
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Abstract

In spite of its limitations, the culture of Cryptosporidium parvum in monolayers of epithelial cells is a suitable model to study the interaction of this protozoan parasite with the host cell, to assay oocyst infectivity, and to screen drugs for anti-cryptosporidial activity. For unknown reasons, growth of Cryptosporidium in culture is limited in time and generally does not lead to the production of significant numbers of oocysts. In monolayers infected with high doses of oocysts, we observed that many cells remain uninfected, suggesting that some cells are less susceptible to the infection. Since C. parvum and the related species C. hominis lack many essential biosynthetic pathways, we tested whether the dependence of the parasite on host cell metabolites may favour the infection of cells in mitosis. The proportion of monolayer cells in stationary (G0/G1) phase and in mitosis (S/G2/M) was determined and the prevalence of infected cells in each subpopulation was quantified. Although C. parvum infects and develops in dividing and stationary cells, a significant preference for cells in S/G2/M phase was observed. Consistent with previous observations showing that C. parvum induces apoptosis in cell monolayers, infection was accompanied by a significant increase in the proportion of mitotic cells.

Keywords

CryptosporidiumCryptosporidium parvummitosisapoptosiscell culture
Type
Research Article
Information
Parasitology , Volume 133 , Issue 2 , August 2006 , pp. 131 - 138
Copyright
2006 Cambridge University Press

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