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Pathways for the influx of molecules into cercariae of Schistosoma mansoni during skin penetration

Published online by Cambridge University Press:  04 February 2010

J. A. THORNHILL ,
P. McVEIGH ,
A. D. JURBERG  and
J. R. KUSEL
J. A. THORNHILL
Affiliation:
Division of Infection and Immunity, Faculty of Biomedical and Life Sciences, Glasgow Biomedical Research Centre, University of Glasgow, 120 University Place, GlasgowG12 8TA, UK
P. McVEIGH
Affiliation:
Biomolecular Processes: Parasitology, School of Biological Sciences, Medical Biology Centre, 97 Lisburn Road, Queen's University Belfast, BelfastBT9 7BL, UK
A. D. JURBERG
Affiliation:
Instituto Gulbenkian de Ciência, Oeiras, Portugal
J. R. KUSEL*
Affiliation:
Division of Infection and Immunity, Faculty of Biomedical and Life Sciences, Glasgow Biomedical Research Centre, University of Glasgow, 120 University Place, GlasgowG12 8TA, UK
*
*Corresponding author: Division of Infection and Immunity, Faculty of Biomedical and Life Sciences, University of Glasgow, Level 5, Glasgow Biomedical Research Centre, 120 University Place, Glasgow G12 8TA, Scotland, UK. Tel: +44 (0)141 330 6968. Fax: +44 (0)141 330 4600. E-mail: [email protected]
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Summary

It has been observed that fluorescent membrane-impermeant molecules can enter the cercariae as they penetrate mouse skin. The hypothesis to be tested was that such molecules, which included Lucifer Yellow and a variety of fluorescent dextrans, entered the parasite through the nephridiopore and excretory tubules as well as through the surface membrane. FITC-labelled poly-L-lysine (molecular weight 10 kDa), added at 4°C during syringe transformation, was found to enter the nephridiopore and labelled the excretory bladder and sometimes the excretory tubules. This finding indicates that macromolecules (10 kDa) can enter the nephridiopore. It was found that linoleic acid (a normal constituent of skin) greatly stimulated uptake of Lucifer Yellow and dextrans into the excretory/subtegumental region of 2-h-old schistosomula. This correlated with an increased uptake of membrane-impermeant propidium iodide at 37°C. Since increased uptake of propidium iodide occurs when membranes become permeable, the surface membrane could also be a pathway of transport of the membrane-impermeant molecules into the schistosomulum.

Keywords

linoleic acidLucifer Yellownephridioporeschistosomulumsurface membrane
Type
Research Article
Information
Parasitology , Volume 137 , Issue 7 , June 2010 , pp. 1089 - 1098
Copyright
Copyright © Cambridge University Press 2010

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