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P19: a female and tissue specifically expressed gene in Schistosoma mansoni, regulated by pairing with the male

Published online by Cambridge University Press:  05 December 2003

A. MICHEL ,
J. KNOBLOCH  and
W. KUNZ
A. MICHEL
Affiliation:
Institute for Genetics, Genetic Parasitology, 40225 Düsseldorf, Germany
J. KNOBLOCH
Affiliation:
Institute for Genetics, Genetic Parasitology, 40225 Düsseldorf, Germany
W. KUNZ
Affiliation:
Institute for Genetics, Genetic Parasitology, 40225 Düsseldorf, Germany
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Abstract

A female-specific sequence was isolated from a cDNA library of Schistosoma mansoni and further characterizedSequences reported here are available from DDBJ/EMBL/GenBank under the Accession number Z27402.. Expression of the corresponding gene (p19) depends on pairing with a male. In situ hybridization and immunohistology experiments revealed exclusive expression of the gene in the cells of the vitellarium, suggesting a function in egg formation. In addition, experimental evidence for cross-linking of the protein under oxidative conditions supports the assumption that the p19 gene may code for an egg-shell precursor protein.

Keywords

Schistosoma mansoniegg-shellmale-female interactionfemale-specific gene expressionpairing-dependent gene expression
Type
Research Article
Information
Parasitology , Volume 127 , Issue 6 , December 2003 , pp. 519 - 524
Copyright
2003 Cambridge University Press

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References

REFERENCES

ALTSCHUL, S. F. & ERICKSON, B. W. ( 1985). Significance of nucleotide sequence alignments: a method for random sequence permutation that preserves dinucleotide and codon usage. Molecular Biology and Evolution 2, 526–538.Google Scholar
BOBEK, L. A., REKOSH, D. M. & LOVERDE, P. T. ( 1991). Schistosoma japonicum: Analysis of eggshell protein genes, their expression, and comparison with similar genes from other schistosomes. Experimental Parasitology 72, 381–390.CrossRefGoogle Scholar
CAPRON, A., RIVEAU, G. J., BARTLEY, P. B. & McMANUS, D. P. ( 2002). Prospects for a schistosome vaccine. Current Drug Targets – Immune Endocrine and Metabolic Disorders 2, 281–290.CrossRefGoogle Scholar
CHEN, L., REKOSH, D. M. & LOVERDE, P. T. ( 1992). Schistosoma mansoni p48 eggshell protein gene: characterization, developmentally regulated expression and comparison to the p14 eggshell protein gene. Molecular and Biochemical Parasitology 52, 39–52.CrossRefGoogle Scholar
DUVALL, R. H. & DEWITT, W. D. ( 1967). An improved perfusion technique for recovering adult schistosomes from laboratory animals. American Journal of Tropical Medicine and Hygiene 16, 483–486.CrossRefGoogle Scholar
FINKEN, M., SOBEK, A., SYMMONS, P. & KUNZ, W. (1994). Characterization of the complete protein disulfide isomerase gene of Schistosoma mansoni and identification of the tissues of its expression. Molecular and Biochemical Parasitology 64, 135–144.CrossRefGoogle Scholar
GREVELDING, C. G. ( 1995). The female-specific W1 sequence of the Puerto Rican strain of Schistosoma mansoni occurs in both genders of a Liberian strain. Molecular and Biochemical Parasitology 71, 269–272.CrossRefGoogle Scholar
GREVELDING, C. G., SOMMER, G. & KUNZ, W. ( 1997). Female-specific gene expression in Schistosoma mansoni is regulated by pairing. Parasitology 115, 635–640.CrossRefGoogle Scholar
KNOBLOCH, J., WINNEN, R., QUACK, M., KUNZ, W. & GREVELDING, C. G. ( 2002). A novel Syk-family tyrosine kinase from Schistosoma mansoni which is preferentially transcribed in reproductive organs. Gene 294, 87–97.CrossRefGoogle Scholar
KÖSTER, B., DARGATZ, H., SCHRÖDER, J., HIRZMANN, J., HAARMANN, C., SYMMONS, P. & KUNZ, W. ( 1988). Identification and localisation of the products of a putative eggshell precursor gene in the vitellarium of Schistosoma mansoni. Molecular and Biochemical Parasitology 31, 183–198.CrossRefGoogle Scholar
KUNZ, W. ( 2001). Schistosome male–female interaction: induction of germ-cell differentiation. Trends in Parasitology 17, 227–231.CrossRefGoogle Scholar
MENRATH, M., MICHEL, A. & KUNZ, W. ( 1995). A female-specific sequence of Schistosoma mansoni encoding a mucin-like protein that is expressed in the epithelial cells of the reproductive duct. Parasitology 111, 477–483.CrossRefGoogle Scholar
REIS, M. G., KUHNS, J., BLANTON, R. & DAVIS, A. H. ( 1989). Localization and pattern of expression of a female specific mRNA in Schistosoma mansoni. Molecular and Biochemical Parasitology 32, 113–120.CrossRefGoogle Scholar
RICE-FICHT, A. C., DUSEK, K. A., KOCHEVAR, G. J. & WAITE, J. H. ( 1992). Eggshell precursor proteins of Fasciola hepatica, I. Structure and expression of vitelline protein B. Molecular and Biochemical Parasitology 54, 129–142.CrossRefGoogle Scholar
ROCHE, C., LIU, J. L., LEPRESLE, T., CAPRON, A. & PIERCE, R. J. ( 1996). Tissue localization and stage-specific expression of the phospholipid hydroperoxide glutathione peroxidase of Schistosoma mansoni. Molecular and Biochemical Parasitology 75, 187–195.CrossRefGoogle Scholar
SAMBROOK, J., FRITSCH, E. F. & MANIATIS, T. ( 1989). Molecular Cloning. A Laboratory Manual, 2nd Edn, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
SCHÜSSLER, P., PÖTTERS, E., WINNEN, R., BOTTKE, W. & KUNZ, W. ( 1995). An isoform of ferritin as a component of protein yolk platelets in Schistosoma mansoni. Molecular Reproduction and Development 41, 325–330.CrossRefGoogle Scholar
WHITFIELD, W. G., MILLAR, S. E., SAUMWEBER, H., FRASCH, M. & GLOVER, D. M. ( 1988). Cloning of a gene encoding an antigen associated with the centrosome in Drosophila. Journal of Cell Science 89, 467–480.Google Scholar
WIPPERSTEG, V., KAPP, K., KUNZ, W., JACKSTADT, P., ZAHNER, H. & GREVELDING, C. G. ( 2002). HSP70-controlled GFP expression in transiently transformed schistosomes. Molecular and Biochemical Parasitology 120, 141–150.CrossRefGoogle Scholar
ZURITA, M., BIEBER, D., RINGOLD, G. & MANSOUR, T. E. ( 1987). Cloning and characterization of a female genital complex cDNA from the liver fluke Fasciola hepatica. Proceedings of the National Academy of Sciences of the USA. 84, 2340–2344.CrossRefGoogle Scholar