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Nuclear size and DNA content in host cells during first-generation schizogony of Eimeria zuernii

Published online by Cambridge University Press:  06 April 2009

J. pasternak ,
M. A. Fernando ,
P. H. G. Stockdale  and
D. Weber
J. pasternak
Affiliation:
Department of Biology, University of Waterloo, waterloo, Ontario, Canada, N2L 3G1.
M. A. Fernando
Affiliation:
Department of pathology, University of Guelph, Guelph, Ontario, Canada, N2G 2W2.
P. H. G. Stockdale
Affiliation:
Animal Diseases Research Institute (W), P. O. Box 640, Lethbridge, Alberta, Canada.
D. Weber
Affiliation:
Department of Biology, University of Waterloo, waterloo, Ontario, Canada, N2L 3G1.
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Extract

The response of intestinal host-cell nuclei of calves infected with Eimeria zuernii 6 and 8 days post-infection was examined using Feulgen-DNA microspectrophotometry. The results show that nuclear hypertrophy is dissociated from DNA replication. In the light of previous work (Fernando, Pasternak, Barrell & Stockdale, 1974) it is surmised that the specificity of infection of cells by E. zuernii is not stringent, with the major target being non-proliferative cells. At most, 20% of the first-generation schizonts develop within cells that would have had proliferative potential and as a result some non-scheduled DNA synthesis occurs.

Type
Research Article
Information
Parasitology , Volume 74 , Issue 2 , April 1977 , pp. 199 - 203
Copyright
Copyright © Cambridge University Press 1977

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References

Beyer, T. V. & Shibalova, T. A. (1974). The increase of DNA quantity in the nuclei of chicken caecal cells harbouring second generation schizonts of Eimeria tenella. Parasitologia (Leningrad) 8, 449–55. (In Russian).Google Scholar
Fernando, M. A. (1974). Fine structure of the schizonts and merozoites of Eimeria acerulina in the chicken. Joutnal of Parasitoloy 60, 149–59.CrossRefGoogle ScholarPubMed
Fernando, M. A., Pasternak, J., Barrell, R. & Stockdale, P. H. G. (1974). Induction of host nuclear DNA synthesis in coccidia-infected chicken intestinal cells. International Journal for Parasitology 4, 267–76.CrossRefGoogle ScholarPubMed
Garcia, A. M. & Iorio, R. (1996). Potential sources of error in two-wavelength cytophotometry. In Introduction to Quantitative Cytochemistry (ed. Wied, G. L.), pp. 215–37. New York and London: Academic Press.Google Scholar
Hammond, D. M. (1971). The development and ecology of coccida and related intracellular parasites. In Ecology and Physiology of Parasites, A Symposium (ed. Fallis, A. M.), pp. 3–19. Toronto: University of Toronto Press.Google Scholar
Pasteernak, J. & Haight, M. (1975). DNA accumulation during oogenesis in the free-living nematode Panagrellus silusiac. Chromosoma (Berlin) 49, 279–98.CrossRefGoogle Scholar
Patau, K. (1952). Absorption Microphotometry of irregular objects. Chromosoma (Berlin) 5, 341–62.CrossRefGoogle Scholar
Sampson, J. R. & Bammond, D. M. (1972). Fine structural aspects of development of Eimeria alabamensis schizonts in cell culture. Journal of Parasitology 58, 311–22.CrossRefGoogle Scholar
Sparrow, A. H., Price, H. J. & Underbrink, A. G. (1972). A Survey of DNA content per cell and per chromosome of prokaryotic and eukaryotic organisms: some evolutionary considerations. In Evolution of Genetic Systems ( ed. Smith, H. H.), pp 451–93. New York: Gordon and Breach.Google Scholar
Stockdale, P. H. G. (1976). Proposed life-cycle of Eimeria zuernii (Rivolta, 1878) Martin 1909. British Veterinary Journal (In the Press).Google Scholar