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Notes on the Collection and Preservation of Parasitic Worms

Published online by Cambridge University Press:  06 April 2009

H. A. Baylis
H. A. Baylis
Affiliation:
(British Museum (Natural History)).
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Extract

At the request of Prof. G. H. F. Nuttall, F.R.S., the writer has attempted to put together a few notes on methods found useful in obtaining well-preserved specimens for helminthological purposes. Of the large number of methods that have been recommended for the various groups by different authorities, only a limited selection can be given, and preference is given, where possible, to those which are comparatively simple and can be employed in the field or under the limited conditions of a field laboratory, while at the same time experience has shown them to give sufficiently good results for ordinary purposes.

Type
Research Article
Information
Parasitology , Volume 14 , Issue 3-4 , December 1922 , pp. 402 - 408
Copyright
Copyright © Cambridge University Press 1922

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References

1 A 1 per cent, solution of salt is sufficiently accurate for practical purposes. In the opinion of some authorities, tap-water is better for intestinal nematodes than salt solution or distilled water. For nematodes from the alimentary canal of fresh-water fishes, Prof. H. B. Ward recommends a 3 per cent, salt solution.

1 The temperature originally recommended by Loose was 80° — 90° C.

2 For travelling purposes, enamelled iron dishes, which can be heated over a spirit lamp, will be found useful.

1 In certain special cases, such as Mermithidae and many of the Filariidae, formalin seems to give better results than alcohol. After very rapid cleaning, these worms may be killed in a 5 per cent, solution of formalin. They may either be kept in this, or transferred to 70 per cent, alcohol by very gradual stages through 30 and 50 per cent, alcohol. It is better not to wash them in water after formalin.

1 Ortlepp, R. J. (1922), Ann. and Mag. Nat. Hist. (9) IX. p. 603.Google Scholar