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A new species of trypanosome, Trypanosoma desterrensis sp. n., isolated from South American bats

Published online by Cambridge University Press:  09 October 2003

E. C. GRISARD ,
N. R. STURM  and
D. A. CAMPBELL
E. C. GRISARD
Affiliation:
Department of Microbiology, Immunology and Molecular Genetics, UCLA School of Medicine, Los Angeles, CA 90095-1747, USA Departamento de Microbiologia e Parasitologia, Universidade Federal de Santa Catarina, Caixa postal 476, 88040-900, Florianópolis, SC, Brazil
N. R. STURM
Affiliation:
Department of Microbiology, Immunology and Molecular Genetics, UCLA School of Medicine, Los Angeles, CA 90095-1747, USA
D. A. CAMPBELL
Affiliation:
Department of Microbiology, Immunology and Molecular Genetics, UCLA School of Medicine, Los Angeles, CA 90095-1747, USA
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Abstract

Trypanosomes isolated from South American bats include the human pathogen Trypanosoma cruzi. Other Trypanosoma spp. that have been found exclusively in bats are not well characterized at the DNA sequence level and we have therefore used the SL RNA gene to differentiate and characterize kinetoplastids isolated from bats in South America. A Trypanosoma sp. isolated from bats in southern Brazil was compared with the geographically diverse isolates T. cruzi marinkellei, T. vespertilionis, and T. dionisii. Analysis of the SL RNA gene repeats revealed size and sequence variability among these bat trypanosomes. We have developed hybridization probes to separate these bat isolates and have analysed the DNA sequence data to estimate their relatedness. A new species, Trypanosoma desterrensis sp. n., is proposed, for which a 5S rRNA gene was also found within the SL RNA repeat.

Keywords

Eptesicus furinalismini-exonspliced leaderSL RNATrypanosoma cruzi marinkelleiTrypanosoma dionisiiTrypanosoma vespertilionis
Type
Research Article
Information
Parasitology , Volume 127 , Issue 3 , September 2003 , pp. 265 - 271
Copyright
© 2003 Cambridge University Press

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References

REFERENCES

AKSOY, S. (1992). Spliced leader RNA and 5S rRNA genes in Herpetomonas spp. are genetically linked. Nucleic Acids Research 20, 913.Google Scholar
BAKER, J. R., MILES, M. A., GODFREY, D. G. & BARRETT, T. (1978). Biochemical characterization of some species of Trypanosoma (Schizotrypanum) from bats (Microchiroptera). American Journal of Tropical Medicine and Hygiene 27, 483–491.CrossRefGoogle Scholar
BOWER, S. M. & WOO, P. T. K. (1981). Two new species of trypanosomes (subgenus Schizotrypanum) in bats from southern Ontario. Canadian Journal of Zoology 59, 530–545.CrossRefGoogle Scholar
CAMPBELL, D. A. (1992). Bodo caudatus medRNA and 5S rRNA genes: tandem arrangement and phylogenetic analyses. Biochemical and Biophysical Research Communications 182, 1053–1058.CrossRefGoogle Scholar
DOLLET, M., STURM, N. R. & CAMPBELL, D. A. (2001). The spliced leader RNA gene array in phloem-restricted plant trypanosomatids (Phytomonas) partitions into two major groupings: epidemiological implications. Parasitology 122, 289–297.CrossRefGoogle Scholar
FERNANDES, O., MURTHY, V. K., KURATH, U., DEGRAVE, W. M. & CAMPBELL, D. A. (1994). Mini-exon gene variation in human pathogenic Leishmania species. Molecular and Biochemical Parasitology 66, 261–271.CrossRefGoogle Scholar
FERNANDES, O., TEIXEIRA, M. M. G., STURM, N. R., SOUSA, M. A., CAMARGO, E. P., DEGRAVE, W. M. & CAMPBELL, D. A. (1997). Mini-exon gene sequences define six groups within the Genus Crithidia. Journal of Eukaryotic Microbiology 44, 535–539.CrossRefGoogle Scholar
FERNANDES, O., STURM, N. R., DERRÉ, R. & CAMPBELL, D. A. (1998). The mini-exon gene: A genetic marker for zymodeme III of Trypanosoma cruzi. Molecular and Biochemical Parasitology 95, 129–133.CrossRefGoogle Scholar
FIGUEIREDO, R. C. B. Q., STEINDEL, M. & SOARES, M. J. (1994). The reservosomes of epimastigote forms of Trypanosoma cruzi: occurrence during in vitro cultivation. Parasitology Research 80, 517–522.CrossRefGoogle Scholar
GIBSON, W., BINGLE, L., BLENDEMAN, W., BROWN, J., WOOD, J. & STEVENS, J. (2000). Structure and sequence variation of the trypanosome spliced leader transcript. Molecular and Biochemical Parasitology 107, 269–277.CrossRefGoogle Scholar
GRISARD, E. C., CAMPBELL, D. A. & ROMANHA, A. J. (1999). Mini-exon sequence polymorphism among Trypanosoma rangeli strains isolated from distinct geographical regions. Parasitology 118, 375–382.CrossRefGoogle Scholar
GRISARD, E. C., STEINDEL, M., SHAW, J. J., ISHIKAWA, I. A. Y., CARVALHO PINTO, C. J., EGER-MANGRICH, I., TOMA, H. K., LIMA, J. H., ROMANHA, A. J. & CAMPBELL, D. A. (2000). Characterization of Leishmania sp. strains isolated from autochthonous cases of human cutaneous leishmaniasis in Santa Catarina State, southern Brazil. Acta Tropica 74, 89–93.Google Scholar
HOARE, C. A. (1972). The Trypanosomes of Mammals: a Zoological Monograph. Blackwell Scientific Publications, Oxford.
JOHNSON, D. A., GAUTSCH, J. W., SPORTSMAN, J. R. & ELDER, J. H. (1984). Improved technique utilizing nonfat dry milk for analysis of proteins and nucleic acids transferred to nitrocellulose. Gene Analysis Techniques 1, 3–8.CrossRefGoogle Scholar
KUMAR, S., TAMURA, K. & NEI, M. (1993). MEGA: Molecular Evolutionary Genetic Analysis. Version 1.02. Institute of Molecular Evolutionary Genetics, The Pennsylvania State University, University Park.
LeCUYER, K. A. & CROTHERS, D. M. (1993). The Leptomonas collosoma spliced leader RNA can switch between two alternate structural forms. Biochemistry 32, 5301–5311.CrossRefGoogle Scholar
MARINKELLE, C. J. (1976). The biology of the trypanosomes of bats. In Biology of the Kinetoplastida (ed. Lumdsen, W. H. R. & Evans, D. A.), pp. 175–216. Academic Press, New York.
MASLOV, D. A., ELGORT, M. G., WONG, S., PECKOVA, H., LOM, J., SIMPSON, L. & CAMPBELL, D. A. (1993). Organization of mini-exon and 5S rRNA genes in the kinetoplastid Trypanoplasma borreli. Molecular and Biochemical Parasitology 61, 127–135.CrossRefGoogle Scholar
MOLYNEUX, D. H. (1991). Trypanosomes of bats. In Parasitic Protozoa (ed. Kreier, J. P. & Baker, J. R. ), pp. 195–223. Academic Press, New York.
MURTHY, V. K., DIBBERN, K. M. & CAMPBELL, D. A. (1992). PCR amplification of mini-exon gene differentiates Trypanosoma cruzi from Trypanosoma rangeli. Molecular Cell Probes 6, 237–243.CrossRefGoogle Scholar
PETRY, K., BALTZ, T. H. & SCHOTTELIUS, J. (1986). Differentiation of Trypanosoma cruzi, T. cruzi marinkellei, T. dionisii and T. vespertilionis by monoclonal antibodies. Acta Tropica 43, 5–13.Google Scholar
SANTANA, D. M., LUKEš, J., STURM, N. R. & CAMPBELL, D. A. (2001). Two sequence classes of kinetoplastid 5S ribosomal RNA gene revealed among Bodonid spliced leader RNA gene arrays. FEMS Microbiology Letters 204, 233–237.CrossRefGoogle Scholar
SOUTO, R. P., FERNANDES, O., MACEDO, A. M., CAMPBELL, D. A. & ZINGALES, B. (1996). DNA markers define two major phylogenetic lineages of Trypanosoma cruzi. Molecular and Biochemical Parasitology 83, 141–152.CrossRefGoogle Scholar
STEINDEL, M., GRISARD, E. C., CARVALHO PINTO, C. J., CORDEIRO, F. D., RIBEIRO-RODRIGUES, R. & ROMANHA, A. J. (1998). Characterization of trypanosomes from the Subgenus Schizotrypanum isolated from bats Eptesicus sp. (Chiroptera: Vespertilionidae), captured in Florianópolis, Santa Catarina State, Brazil. Journal of Parasitology 84, 601–607.Google Scholar
STEVENS, J. R. & GIBSON, W. (1999). The molecular evolution of Trypanosomes. Parasitology Today 15, 432–437.CrossRefGoogle Scholar
STEVENS, J. R., NOYES, H. A., DOVER, G. A. & GIBSON, W. C. (1999 a). The ancient and divergent origins of the human pathogenic trypanosomes, Trypanosoma brucei and T. cruzi. Parasitology 118, 107–116.Google Scholar
STEVENS, J. R., TEIXEIRA, M. M. G., BINGLE, L. E. H. & GIBSON, W. C. (1999 b). The taxonomic position and evolutionary relationships of Trypanosoma rangeli. International Journal for Parasitology 29, 749–757.Google Scholar
STURM, N. R., FERNANDES, O. & CAMPBELL, D. A. (1995). The mini-exon genes of three Phytomonas isolates that differ in plant tissue tropism. FEMS Microbiology Letters 130, 177–182.CrossRefGoogle Scholar
STURM, N. R., MURTHY, V. K., GARSIDE, L. & CAMPBELL, D. A. (1998). The mini exon gene of Trypanosoma (Nannomonas) simiae: sequence variation between isolates as a distinguishing molecular marker. Acta Tropica 71, 199–206.CrossRefGoogle Scholar
STURM, N. R., YU, M. C. & CAMPBELL, D. A. (1999). Transcript termination and 3′-end processing of the Spliced Leader RNA in kinetoplastids. Molecular and Cellular Biology 19, 1595–1601.CrossRefGoogle Scholar
STURM, N. R., MASLOV, D. A., GRISARD, E. C. & CAMPBELL, D. A. (2001). Diplonema spp. posses spliced leader RNA genes typical of the Kinetoplastida. Journal of Eukaryotic Microbiology 48, 325–331.Google Scholar
TEIXEIRA, L. F. M., GONÇALVES, A. M., ROMANHA, A. J., STEINDEL, M. & PINTO, A. S. (1993). Schizodeme and zymodeme analysis of trypanosomes of the subgenus Schizotrypanum from the bat. Parasitology Research 79, 497–500.CrossRefGoogle Scholar
THOMPSON, J. D., GIBSON, T. J., PLEWNIAK, F., JEANMOUGIN, F. & HIGGINS, D. G. (1997). The CLUSTALX windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Research 25, 4876–4882.CrossRefGoogle Scholar
TIBAYRENC, M., NEUBAUER, K., BARNABE, C., GUERRINI, F., SKARECKY, D. & AYALA, F. (1993). Genetic characterization of six parasitic protozoa: Parity between random primer DNA typing and multilocus enzyme electrophoresis. Proceedings of the National Academy of Sciences, USA 90, 1335–1339.CrossRefGoogle Scholar
ZUKER, M., MATHEWS, D. H. & TURNER, D. H. (1999). Algorithms and thermodynamics for RNA secondary structure prediction: a practical guide. In RNA Biochemistry and Biotechnology (ed. Barciszewski, J. & Clark, B. F. C.), pp. 11–43. Kluwer Academic Publishers, Amsterdam.CrossRef