Hostname: page-component-cd9895bd7-dzt6s Total loading time: 0 Render date: 2024-12-25T01:22:31.216Z Has data issue: false hasContentIssue false
  • English
  • Français

Nematicidal effects of cysteine proteinases against sedentary plant parasitic nematodes

Published online by Cambridge University Press:  19 July 2007

G. STEPEK ,
R. H. C. CURTIS ,
B. R. KERRY ,
P. R. SHEWRY ,
S. J. CLARK ,
A. E. LOWE ,
I. R. DUCE ,
D. J. BUTTLE  and
J. M. BEHNKE
G. STEPEK
Affiliation:
School of Biology, University of Nottingham, Nottingham NG7 2RD, UK
R. H. C. CURTIS*
Affiliation:
Rothamsted Research, Harpenden, Herts AL5 2JQ, UK
B. R. KERRY
Affiliation:
Rothamsted Research, Harpenden, Herts AL5 2JQ, UK
P. R. SHEWRY
Affiliation:
Rothamsted Research, Harpenden, Herts AL5 2JQ, UK
S. J. CLARK
Affiliation:
Rothamsted Research, Harpenden, Herts AL5 2JQ, UK
A. E. LOWE
Affiliation:
School of Biology, University of Nottingham, Nottingham NG7 2RD, UK
I. R. DUCE
Affiliation:
School of Biology, University of Nottingham, Nottingham NG7 2RD, UK
D. J. BUTTLE
Affiliation:
Academic Unit of Molecular Medicine, University of Sheffield, Sheffield S10 2RX, UK
J. M. BEHNKE
Affiliation:
School of Biology, University of Nottingham, Nottingham NG7 2RD, UK
*
*Corresponding author: Division of Plant Pathogen Interactions, Rothamsted Research, Harpenden, Herts AL5 2JQ, UK. Tel: 44 1582 763 133 2437. Fax: 44 1582 760 981. E-mail: [email protected]
Get access Rights & Permissions [Opens in a new window]

Summary

Cysteine proteinases from the fruit and latex of plants, such as papaya, pineapple and fig, have previously been shown to have substantial anthelmintic efficacy, in vitro and in vivo, against a range of animal parasitic nematodes. In this paper, we describe the in vitro effects of these plant extracts against 2 sedentary plant parasitic nematodes of the genera Meloidogyne and Globodera. All the plant extracts examined caused digestion of the cuticle and decreased the activity of the tested nematodes. The specific inhibitor of cysteine proteinases, E-64, blocked this activity completely, indicating that it was essentially mediated by cysteine proteinases. In vitro, plant cysteine proteinases are active against second-stage juveniles of M. incognita and M. javanica, and some cysteine proteinases also affect the second-stage juveniles of Globodera rostochiensis. It is not known yet whether these plant extracts will interfere with, or prevent invasion of, host plants.

Keywords

plant parasitic nematodesMeloidogyne sppGlobodera rostochiensisplant cysteine proteinasesin vitronematicide
Type
Research Article
Information
Parasitology , Volume 134 , Issue 12 , November 2007 , pp. 1831 - 1838
Copyright
Copyright © Cambridge University Press 2007

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

Ahmad, M. S., Mukhtar, T. and Ahmad, R. (2004). Some studies on the control of citrus nematode (Tylenchus semipenetrans) by leaf extracts of three plants and their effects on plant growth variables. Asian Journal of Plant Sciences 3, 544–548.Google Scholar
Akhtar, M. and Mahmood, I. (1994). Potentiality of phytochemicals in nematode control: a review. Bioresource Technology 48, 189–201.Google Scholar
Berger, J. and Asenjo, C. F. (1939). Anthelmintic activity of fresh pineapple juice. Science 90, 299–300.CrossRefGoogle Scholar
Berger, J. and Asenjo, C. F. (1940). Anthelmintic activity of crystalline papain. Science 91, 387–388.Google Scholar
Bird, A. F. and Bird, J. (1991). The Egg. In The Structure of Nematodes (ed. Bird, A. F.), pp. 7–43. Academic Press, New York.Google Scholar
Chitwood, D. J. (2003). Research on plant-parasitic nematode biology conducted by the United States Department of Agriculture – Agricultural Research Service. Pest Management Science 59, 748–753.Google Scholar
Dubois, T., Jacquet, A., Schnek, A. G. and Looze, Y. (1988). The thiol proteinases from the latex of Carica papaya L. I. Fractionation, purification and preliminary characterisation. Biological Chemistry Hoppe-Seyler 369, 733–740.Google Scholar
Eisenbeck, J. D. (1985). Detailed morphology and anatomy of second-stage juveniles, males and females of the genus Meloidogyne (root-knot nematodes). In An Advanced Treatise on Meloidogyne. Vol. I: Biology and Control (ed. Sasser, J. N. and Carter, C. C.), pp. 47–77. Department of Plant Pathology and the United States Agency for International Development.Google Scholar
Fioretti, L., Porter, A., Haydock, P. and Curtis, R. H. C. (2002). Monoclonal antibodies reactive with secreted-excreted products from the amphids and the cuticle surface of Globodera pallida affect nematode movement and delay invasion of potato plants. International Journal for Parasitology 32, 1709–1718.Google Scholar
Giday, M., Asfaw, Z., Elmqvist, T. and Woldu, Z. (2003). An ethnobotanical study of medicinal plants used by the Zay people in Ethiopia. Journal of Ethnopharmacology 85, 43–52.Google Scholar
Hussey, R. S. and Barker, K. R. (1973). A comparison of methods of collecting inocula of Meloidogyne spp. including a new technique. Plant Disease Report 57, 1025–1028.Google Scholar
Kowlessur, D., O'Driscol, M., Topham, M., Templeton, W., Thomas, E. W. and Brocklehurst, K. (1989). The interplay of electrostatic fields and binding interactions determining catalytic-site reactivity in actinidin. The Biochemical Journal 259, 443–452.Google Scholar
McDowall, M. A. (1970). Anionic proteinase from Actinidia chinensis: preparation and properties of the crystalline enzyme. European Journal of Biochemistry 14, 214–221.Google Scholar
Meyer, S. L. F. (2003). United States Department of Agriculture – Agricultural Research Service research programs on microbes for management of plant-parasitic nematodes. Pest Management Science 59, 665–670.Google Scholar
Miller, P. M. and Sands, D. C. (1977). Effects of hydrolytic enzymes on plant-parasitic nematodes. Journal of Nematology 9, 192–197.Google Scholar
Peanasky, R. J. and Abu-Erreish, G. (1970). Inhibitors from Ascaris lumbricoides: interactions with the host's digestive enzymes. In Proceedings of the International Research Conference on Proteinase Inhibitors, Berlin, 1970. pp. 281–293.Google Scholar
Robbins, B. H. (1930). A proteolytic enzyme in ficin, the anthelmintic principle of Leche de Higueron. Journal of Biological Chemistry 87, 251–257.Google Scholar
Rowan, A. D., Buttle, D. J. and Barrett, A. J. (1990). The cysteine proteinases of the pineapple plant. The Biochemical Journal 266, 869–875.Google Scholar
Sgarbieri, V. C., Gupte, S. M., Kramer, D. E. and Whitaker, J. R. (1964). Ficus enzymes. I: Separation of the proteolytic enzymes of Ficus carica and Ficus glabrata latices. Journal of Biological Chemistry 239, 2170–2177.CrossRefGoogle Scholar
Sharon, E., Spiegel, Y., Solomon, R. and Curtis, R. H. C. (2002). Characterisation of Meloidogyne javanica surface coat using antibodies and their effect on nematode behaviour. Parasitology 125, 177–185.CrossRefGoogle Scholar
Siddiqui, M. A., Haseeb, A. and Alam, M. (1992). Control of plant-parasitic nematodes by soil amendments with latex bearing plants. Indian Journal of Nematology 22, 25–28.Google Scholar
Stepek, G., Buttle, D. J., Duce, I. R., Lowe, A. and Behnke, J. M. (2005). Assessment of the anthelmintic effect of natural plant cysteine proteinases against the gastrointestinal nematode, Heligmosomoides polygyrus, in vitro. Parasitology 130, 203–211.Google Scholar
Stepek, G., Lowe, A. E., Buttle, D. J., Duce, I. R. and Behnke, J. M. (2006). In vitro and in vivo anthelmintic efficacy of plant cysteine proteinases against the rodent gastrointestinal nematode, Trichuris muris. Parasitology 132, 681–689.Google Scholar
Stepek, G., Lowe, A. E., Buttle, D. J., Duce, I. R. and Behnke, J. M. (2007). Anthelmintic action of plant cysteine proteinases against the rodent stomach nematode, Protospirura muricola, in vitro and in vivo. Parasitology 134, 103–112.CrossRefGoogle Scholar
Zang, X. and Maizels, R. M. (2001). Serine proteinase inhibitors from nematodes and the arms race between host and pathogen. Trends in Biochemical Sciences 26, 191–197.CrossRefGoogle Scholar
Zunke, U. and Eisenbeck, J. D. (1998). Morphology and ultrastructure. In The Cyst Nematodes (ed. Sharma, S. B.), pp. 31–56. Chapman & Hall, London.CrossRefGoogle Scholar