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Necator americanus in inbred mice: a re-evaluation of primary infection kinetics

Published online by Cambridge University Press:  06 April 2009

L. M. Timothy  and
J. M. Behnke
L. M. Timothy
Affiliation:
MRC Experimental Parasitology Group, Department of Life Science, University of Nottingham, Nottingham NG7 2RD, UK
J. M. Behnke
Affiliation:
MRC Experimental Parasitology Group, Department of Life Science, University of Nottingham, Nottingham NG7 2RD, UK
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Summary

The course of a primary Necator americanus infection was studied in the lungs and small intestines of syngeneic mice. Following percutaneous infection no difference in initial larval establishment in the lungs was found between male BALB/c, NIH or B10.G mice. However, significant differences in the subsequent kinetics of infection were demonstrated between the BALB/c and NIH strains. Lung worm burdens declined more slowly in NIH mice than in BALB/c strain. Surprisingly, however, a greater proportion of larvae remaining in the lungs of BALB/c mice, 9 days p.i., were trapped than in NIH mice. Nevertheless, establishment in the small intestines of the BALB/c strain was consistently greater than in NIH mice. Host immunosuppression resulted in increased larval retention in the lungs of both the BALB/c and NIH strains as well as in the small intestines of BALB/c mice. Treatment with hydrocortisone acetate did not increase intestinal worm burdens in NIH mice. The data presented suggest that, in this complex, dynamic model system, designation of ‘susceptible’ and ‘resistant’ strains is inappropriate. The factors underlying the observed strain differences in resistance to infection are discussed.

Keywords

Necator americanushookwormmodelmiceinfection kinetics
Type
Research Article
Information
Parasitology , Volume 107 , Issue 4 , November 1993 , pp. 425 - 431
Copyright
Copyright © Cambridge University Press 1993

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References

REFERENCES

Bartlett, A. & Ball, P. A. J. (1972). Nematospiroides dubius in the mouse as a possible model of endemic human hookworm infection. Annals of Tropical Medicine and Parasitology 66, 129–34.CrossRefGoogle ScholarPubMed
Behnke, J. M., Paul, V. & Rajasekariah, G. R. (1986 a). The growth and migration of Necator americanus following infection of neonatal hamsters. Transactions of the Royal Society of Tropical Medicine and Hygiene 80, 146–9.CrossRefGoogle ScholarPubMed
Behnke, J. M. & Pritchard, D. I. (1987). Necator americanus in neonatally infected hamsters. The timecourse of infection and antibody response to the surface antigens of L4 and adult worms. Transactions of the Royal Society of Tropical Medicine and Hygiene 81, 967–72.Google Scholar
Behnke, J. M., Wells, C. & Brown, J. (1986 b). An improved technique for experimental infections with skin penetrating nematode larvae (Necator americanus). International Journal for Parasitology 16, 461–4.CrossRefGoogle ScholarPubMed
Bhopale, M. K., Kamath, V. R. & Menon, S. (1984). Necator americanus in infant rabbit model and its infectivity in other hosts. Helminthologia 21, 115–21.Google Scholar
Bhopale, M. K., Menon, S. & Kulkarni, L. (1980). Necator americanus in infant rabbits: complete development, humoral antibody, leucocyte response and serum protein changes following infection. Journal of Helminthology 54, 97–104.CrossRefGoogle ScholarPubMed
Bhopale, M. K., Menon, S. & Renapurkar, D. M. (1977). Infant rabbit–a suitable laboratory model for human hookworm (Necator americanus) infection. Bulletin of the Haffkine Institute 5, 38–41.Google Scholar
Carroll, S. M. (1990). Immunity: hookworm in animal model systems. In Hookworm Disease: Current Status and New Directions (ed. Schad, G. A. & Warren, K. S.), pp. 391–403. London: Taylor & Francis.Google Scholar
Meddis, R. (1984). Statistics Using Ranks: a Unified Approach. Oxford: Basil Blackwell.Google Scholar
Orihel, T. C. (1971). Necator americanus in primates. Journal of Parasitology 57, 117–21.CrossRefGoogle ScholarPubMed
Schad, G. A. & Anderson, R. M. (1985). Predisposition to hookworm infection in humans. Science 228, 1537–40.CrossRefGoogle ScholarPubMed
Sen, H. G. (1972). Necator americanus: behaviour in hamsters. Experimental Parasitology 32, 26–32.CrossRefGoogle ScholarPubMed
Sen, H. G., Joshi, U. N. & Seth, D. (1965). Effect of cortisone upon Ancylostoma caninum infection in albino mice. Transactions of the Royal Society of Tropical Medicine and Hygiene 59, 684–9.Google Scholar
Sen, H. G. & Seth, D. (1967). Complete development of the human hookworm, Necator americanus in golden hamsters, Mesocricetus auratus. Nature, London 214, 609–10.CrossRefGoogle Scholar
Sen, H. G. & Seth, D. (1970). Development of Necator americanus in the golden hamsters, Mesocricetus auratus. Indian Journal of Medical Research 58, 1356–60.Google ScholarPubMed
Srilakshmi, N., Lakshmi, P. N., Vardhani, V. V. & Johri, G. N. (1982). Effect of immunosuppressants in mice. Part I. Promethazine hydrochloride and hydrocortisone acetate treatments during experimental infections of Ancylostoma caninum and Nematospiroides dubius. Indian Journal of Experimental Biology 20, 117–20.Google Scholar
Timothy, L. M., Coulson, P. S., Behnke, J. M. & Wilson, R. A. (1992). Cross-reactivity between Necator americanus and Schistosoma mansoni in mice. International Journal for Parasitology 22, 1143–9.Google Scholar
Wahid, F. N., Robinson, M. & Behnke, J. M. (1989). Immunological relationships during primary infection with Heligmosomoides polygyrus (Nematospiroides dubius): expulsion of adult worms from fast responder syngeneic and hybrid strains of mice. Parasitology 98, 459–69.Google Scholar
Wakelin, D. (1985). Genetic control of immunity to helminth infections. Parasitology Today 1, 17–23.Google Scholar
Wells, C. (1988). A study of Necator americanus in rodents. Ph.D. thesis, University of Nottingham.Google Scholar
Wells, C. & Behnke, J. M. (1988 a). Acquired resistance to the human hookworm Necator americanus in mice. Parasite Immunology 10, 493–505.CrossRefGoogle Scholar
Wells, C. & Behnke, J. M. (1988 b). The course of primary infection with Necator americanus in syngeneic mice. International Journal for Parasitology 18, 47–51.Google Scholar
Wilkinson, M. J., Wells, C. & Behnke, J. M. (1990). Necator americanus in the mouse: histopathological changes associated with the passage of larvae through the lungs of mice exposed to primary and secondary infection. Parasitology Research 76, 386–92.Google Scholar