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Natural IgM antibodies in sera from various animals but not the cat kill Toxoplasma gondii by activating the classical complement pathway

Published online by Cambridge University Press:  01 March 2004

Y. KANEKO ,
Y. TAKASHIMA ,
X. XUAUN ,
I. IGARASHI ,
H. NAGASAWA ,
T. MIKAMI  and
H. OTSUKA
Y. KANEKO
Affiliation:
Department of Global Agricultural Sciences, Graduate School of Agricultural and Life Sciences, University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan
Y. TAKASHIMA
Affiliation:
Department of Global Agricultural Sciences, Graduate School of Agricultural and Life Sciences, University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan
X. XUAUN
Affiliation:
National Research Center for Protozoan Disease, Obihiro University of Agricultural and Veterinary Medicine, Inada-Cho, Obihiro, Hokkaido 080-8555, Japan
I. IGARASHI
Affiliation:
National Research Center for Protozoan Disease, Obihiro University of Agricultural and Veterinary Medicine, Inada-Cho, Obihiro, Hokkaido 080-8555, Japan
H. NAGASAWA
Affiliation:
National Research Center for Protozoan Disease, Obihiro University of Agricultural and Veterinary Medicine, Inada-Cho, Obihiro, Hokkaido 080-8555, Japan
T. MIKAMI
Affiliation:
Nihon University College of Bioresource Sciences, 1866 Kameino, Fujisawa 252-8510, Japan
H. OTSUKA
Affiliation:
Department of Global Agricultural Sciences, Graduate School of Agricultural and Life Sciences, University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan
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Abstract

Sera from swine, rabbit, and dog, that had never been exposed to Toxoplasma gondii, demonstrated significant killing of T. gondii tachyzoites in vitro, while cat serum did not. Swine and rabbit sera contained natural IgM antibody against the tachyzoites, and the classical complement pathway was activated by the binding of natural IgM antibody to the tachyzoites, leading to lysis. Anti-T. gondii antibodies, induced in swine or cat infected with T. gondii, had no killing effect by themselves but killed the tachyzoites in the presence of swine complement. However, the anti-T. gondii antibodies of swine or cat demonstrated a very low killing effect in the presence of cat complement. This suggests that T. gondii tachyzoites have an evasion mechanism to prevent lysis which is specific for cat complement.

Keywords

Toxoplasma gondiicomplementclassical pathwaynatural antibody
Type
Research Article
Information
Parasitology , Volume 128 , Issue 2 , February 2004 , pp. 123 - 129
Copyright
2004 Cambridge University Press

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