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Morphological features of spermatozoa of Paragonimus ohirai (Trematoda: Platyhelminthes) examined by a silver nitrate staining technique

Published online by Cambridge University Press:  06 April 2009

H. Hirai  and
I. Tada
H. Hirai
Affiliation:
Department of Parasitic Diseases, Kumamoto University Medical School, 2-2-1 Honjo, Kumamoto, 860, Japan
I. Tada
Affiliation:
Department of Parasitic Diseases, Kumamoto University Medical School, 2-2-1 Honjo, Kumamoto, 860, Japan
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Extract

The mature thread-like spermatozoon of Paragonimus ohirai was shown to be composed of 7 distinct segments which have morphologically different characteristics, following staining with silver nitrate. The first segment (1S) is a needle-shaped structure with a perforatorium. The 2S is composed of two filaments loosely paired; 3S consists of a somewhat coarse thread (vestige of the cytoplasmic projection); 4S is composed of two flagella tightly paired; in 5S, two silver-positive filaments are independently seen as in 2S; 6S has a ‘spiral’ structure composed of two flagella; in 7S, two filaments are seen dissociated from each other. The sperm nucleus exists within the ‘spiral’ structure of 6S and this structure may serve to hold and/or protect the nucleus. The technique presented here is useful for gross morphological observations of the long sperm of flatworms.

Keywords

Paragonimus ohiraidigeneasilver nitrate stainingspermatozoonspermiogenesissperm nucleus
Type
Research Article
Information
Parasitology , Volume 103 , Issue 1 , August 1991 , pp. 103 - 110
Copyright
Copyright © Cambridge University Press 1991

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