Hostname: page-component-586b7cd67f-tf8b9 Total loading time: 0 Render date: 2024-12-01T01:20:17.391Z Has data issue: false hasContentIssue false
  • English
  • Français

A morphological and histochemical study of egg formation in a cyclophyllidean cestode

Published online by Cambridge University Press:  06 April 2009

L. N. Johri
L. N. Johri
Affiliation:
The work concerned in this paper was carried out in the Department of Experimental Biology, Trinity College, Dublin
Get access Rights & Permissions [Opens in a new window]

Extract

1. The morphology and histochemistry of the genitalia concerned in the formation of the egg in Multiceps smyihi n.sp. are described.

2. The vitelline cells are devoid of the phenolic protein globules, which occur in the vitelline cells of the Pseudophyllidea; this result is correlated with the absence of a true external egg-shell.

3. The hardened embryophore (often mistakenly termed the egg-shell) gives none of the reactions of sclerotin (tanned protein) or chitin, but gives a strong P.F.A.S. reaction for disulphide bonds, suggesting that it is a ‘keratin’ type of protein.

4. The cells of Mehlis' gland secrete a material which is intensely P.A.S.-positive and is not digested by saliva, α-amylase or hyaluronidase. This suggests that it may be secreting a mucopolysaccharide or muco- or glycoprotein.

Type
Research Article
Information
Parasitology , Volume 47 , Issue 1-2 , June 1957 , pp. 21 - 29
Copyright
Copyright © Cambridge University Press 1957

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Brown, C. H. (1950). Keratins in invertebrates. Nature, Lond., 166, 439.CrossRefGoogle ScholarPubMed
Brown, C. H. (1952). Some structural proteins of Mytilus edulis. Quart. J. Micro. Sci. 93, 487–503.Google Scholar
Brunet, P. C. J. (1951). The formation of the ootheca by Periplaneta americana. I. The micro-anatomy and histology of the posterior part of the abdomen. Quart. J. Micr. Sci. 92, 113–27.Google Scholar
Brunet, P. C. (1952). The formation of the ootheca by Periplaneta americana. II The structure and function of the left colleterial gland. Quart. J. Micr. Sci. 93, 47–69.Google Scholar
Brunet, P. C. J. & Kent, P. W. (1955). Mechanism of sclerotin formation: the participation of a beta-glucoside. Nature, Lond., 175, 819–820.CrossRefGoogle ScholarPubMed
Dawes, B. (1946). The Trematoda. Cambridge University Press.Google Scholar
Johri, L. N. & Smyth, J. D. (1955). Unpublished work.Google Scholar
Mazia, D., Brewer, P. & Alfert, M. (1953). Cytochemical staining and measurement of protein with bromophenol blue. Biol. Bull., Woods Hole, 104, 57–67.CrossRefGoogle Scholar
Nurse, F. R. (1950). Quinone tanning in the cocoon shell of Dendrocoelum lacteum. Nature, Lond., 165, 570.CrossRefGoogle Scholar
Pearse, A. G. E. (1953). Histochemistry. Churchill, London.Google ScholarPubMed
Pryor, M. G. M. (1940). On the hardening of the ootheca of Blatta. Proc. Roy. Soc. B, 128, 373.Google Scholar
Pryor, M. G. M. (1955). Tanning of blowfly puparia. Nature, Lond., 175, 600.CrossRefGoogle Scholar
Rennison, B. D. (1953). A morphological & histochemical study of egg-shell formation in Dielidophora merlangi. M.Sc. Thesis, Univ. of Dublin (unpublished).Google Scholar
Romanini, M. G. (1947). Contribute alia conoscenza istochimica dei vitellogeni di Distoma hepaticum. Monit. zool. ital. 56, 16–19.Google Scholar
Serra, J. A. (1946). Histochemical tests for proteins and amino acids; the characterization of basic proteins. Stain Tech. 21, 5–18.Google ScholarPubMed
Smyth, J. D. (1954). A technique for the histochemical demonstration of polyphenol oxidase and its application to egg-shell formation in helminths and bussus formation. Mytilus. Quart. J. Micr. Sci. 95, 139–52.Google Scholar
Smyth, J.D. (1956). Studies on tapeworm physiology. IX. A histochemical study of egg-shell formation in Schistocephalus solidus (Pseudophyllidea). Exp. Parasitol. 5, 519–40.CrossRefGoogle Scholar
Stephenson, W. (1947). Physiological and histochemical observations on the adult liver fluke, Fasciola hepatica L. III. Egg-shell formation. Parasitology, 38, 128–39.CrossRefGoogle Scholar
Valcurone, M. (1953). Ricerche istochimiche sui granuli vitellini dei Policladi. Arch. zool. (ital.), Napoli, 38, 245–67.Google Scholar
Vialli, M. (1933). Ricerche istochimiche sui vitellogeni dei platelminti. Boll. Zool. 4, 135–8.CrossRefGoogle Scholar
Vialli, M. (1934). Ricerche istochimiche sui granuli vitellini dei policladi. Boll. Zool. 5, 21–3.CrossRefGoogle Scholar
Yosufzai, H. K. (1953). Shell gland and egg-shell formation in Fasciola hepatica L. Parasitology, 43, 88–93.CrossRefGoogle Scholar