Hostname: page-component-78c5997874-8bhkd Total loading time: 0 Render date: 2024-11-06T09:03:37.377Z Has data issue: false hasContentIssue false
  • English
  • Français

Monoclonal antibodies against surface antigens of schistosomula of Schistosoma mansoni

Published online by Cambridge University Press:  06 April 2009

D. W. Taylor  and
A. F. Butterworth
D. W. Taylor
Affiliation:
Department of Pathology, Tennis Court Road, Cambridge
A. F. Butterworth
Affiliation:
Department of Pathology, Tennis Court Road, Cambridge
Get access Rights & Permissions [Opens in a new window]

Summary

Monoclonal antibodies have been produced after fusion of NS-1 murine myeloma cells with spleen cells from mice immunized either by chronic primary infection or with irradiated cercariae: in both cases, animals were challenged with live cercariae 7 days before fusion. The initial cultures were screened for anti-schistosomular antibodies both by a radioimmunoassay with whole schistosomulum extracts and by immunofluorescence. There was no correlation between the two techniques and subsequent screening was carried out by immunofluorescence. Cloning was carried out in soft agar and 7 cloned cell lines, from 5 initial cultures, were selected for detailed study. Products of 6 of these 7 lines were monoclonal, as judged by isoelectricfocusing of [35S]methionine-labelled supernatant fluids, and their binding to live schistosomula was specific. None of the antibodies showed detectable activity in mediating eosinophil- or complement-dependent damage to schistosomula in vitro. However, 2 antibodies were successfully used to isolate surface proteins with an apparent molecular weight of 24000 on SDS-polyacrylamide gel electrophoresis.

Type
Research Article
Information
Parasitology , Volume 84 , Issue 1 , February 1982 , pp. 65 - 82
Copyright
Copyright © Cambridge University Press 1982

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

Bickle, Q. D., Taylor, M. G., Doenhoff, M. J. & Nelson, G. S. (1979). Immunization of mice with gamma-irradiated intramuscularly injected schistosomula of Schistosoma mansoni. Parasitology 79, 209–22.CrossRefGoogle ScholarPubMed
Bolton, A. E. & Hunter, W. J. (1973). The labelling of proteins to high specific radioactivity by conjugation to a 125I-containing acylating reagent. The Biochemical Journal 133, 529–38.CrossRefGoogle Scholar
Bout, D., Rousseaux, R., Carlier, Y. & Capron, A. (1980). Kinetics of class and sub-class of total immunoglobulins and specific antibodies to Schistosoma mansoni during murine infection. Parasitology 80, 247–56.CrossRefGoogle Scholar
Butterworth, A. E. (1977). Effector mechanisms against schistosomes in vitro. American Journal of Tropical Medicine and Hygiene 26 (Suppl.), 29–38.CrossRefGoogle ScholarPubMed
Butterworth, A. E., Taylor, D. W., Veith, M. C., Vadas, M. A., Dessein, A., Sturrock, R. F. & Wells, E. (1981). Studies on the mechanisms of immunity in human schistosomiasis. Immunological Reviews (in the Press).Google Scholar
Clegg, J. A. & Smithers, S. R. (1972). The effect of immune rhesus monkey serum on schistosomula of Schistosoma mansoni during cultivation in vitro. International Journal for Parasitology 2, 79–98.CrossRefGoogle ScholarPubMed
David, J. R., Vadas, M. A., Butterworth, A. E., De Brito, P. A., Carvalho, E. M., David, R. A., Bina, J. C. & Andrade, Z. A. (1980). Enhanced helminthotoxic capacity of eosinophils from patients with eosinophilia. New England Journal of Medicine 303, 1147–52.CrossRefGoogle ScholarPubMed
Ey, P. L., Prowse, S. J. & Jenkin, C. R. (1978). Isolation of pure IgG1, IgG2a and IgG2b immunoglobulins from mouse serum using protein A-sepharose. Immunochemistry 15, 429–36.CrossRefGoogle ScholarPubMed
Howard, J. C., Butcher, G. W., Galfre, G. & Milstein, C. (1979). Monoclonal anti-rat MHC (H-1) alloantibodies. Current Topics in Microbiology and Immunology 81, 54–60.Google Scholar
Köhler, G. & Milstein, C. (1975). Continuous cultures of fused cells secreting antibody of predefined specificity. Nature, London 256, 495–7.CrossRefGoogle ScholarPubMed
Köhler, G. & Milstein, C. (1976). Derivation of specific antibody-producing tissue culture and tumor lines by cell fusion. European Journal of Immunology 6, 511–19.CrossRefGoogle ScholarPubMed
Köhler, G., Howe, S. C. & Milstein, C. (1976). Fusion between immunoglobulin-secreting and non-secreting myeloma cells. European Journal of Immunology 6, 292–5.CrossRefGoogle Scholar
Laemmli, U. K. (1970). Cleavage of structural proteins during assembly of the head of the bacteriophage T4. Nature, London, 227, 680–5.CrossRefGoogle ScholarPubMed
Laskey, R. A. (1980). The use of intensifying screens or organic scintillators for visualizing radioactive molecules resolved by gel electrophoresis. In Methods in Enzymology vol. 65, (ed. Grossman, L. and Moldane, K.), pp. 363–70. London: Academic Press.Google Scholar
Miller, K. L. & Smithers, S. R. (1980). Schistosoma mansoni: the attrition of a challenge infection in mice immunized with highly irradiated live cercariae. Experimental Parasitology 50, 212–21.CrossRefGoogle ScholarPubMed
Oi, V. T., Jones, P. P., Goding, J. W., Herzenberg, L. A. & Herzenberg, L. A. (1979). Properties of monoclonal antibodies to mouse Ig allotypes, H-2, and Ia antigens. Current Topics in Microbiology and Immunology 81, 115–29.Google Scholar
Phillips, S. M. & Colley, D. G. (1978). Immunologic aspects of host responses to schistosomiasis: resistance, immunopathology, and eosinophil involvement. Progress in Allergy 24, 49–182.Google ScholarPubMed
Ramalho-Pinto, F. J., de Rossi, R. & Smithers, S. R. (1979). Murine schistosomiasis mansoni: antischistosomular antibodies and the IgG subclasses involved in the complement- and eosinophil-mediated killing of schistosomula in vitro. Parasite Immunology 1, 295–308.CrossRefGoogle Scholar
Rowe, D. S. (1980). The role of monoclonal antibody technology in immunoparasitology. Immunology Today 1, 30–3.CrossRefGoogle ScholarPubMed
Sher, A., Smithers, S. R., Mackenzie, P. & Broomfield, K. (1977). Schistosoma mansoni: immunoglobulins involved in passive immunization of laboratory mice. Experimental Parasitology 41, 160–6.CrossRefGoogle ScholarPubMed
Smithers, S. R. & Terry, R. J. (1976). The immunology of schistosomiasis. Advances in Parasitology 14, 399–422.CrossRefGoogle ScholarPubMed
Stanworth, D. R. & Turner, M. W. (1979). Immunochemical analysis of immunoglobulins and their subunits. In Handbook of Experimental Immunology (ed. Weir, D. M.), 3rd edn., pp. 6.1–6.120. Oxford: Blackwell.Google Scholar
Taylor, D. W., Hayunga, E. G. & Vannier, W. E. (1981). Surface antigens of Schistosoma mansoni. Molecular and Biochemical Parasitology 3, 157–68.CrossRefGoogle ScholarPubMed
Torpier, G., Capron, A. & Ouaissi, M. A. (1979). Receptor for IgG (Fc) and human B2-microglobulin on S. mansoni schistosomula. Nature, London 278, 447–9.CrossRefGoogle Scholar
Tsu, T. T. & Herzenberg, L. A. (1980). Solid-phase radioimmune assays. In Selected Methods in Cellular Immunology, (ed. Mishell, B. B. and Shiigi, S. M.), pp. 373–397. San Francisco: W. H. Freeman.Google Scholar
Vadas, M. A., David, J. R., Butterworth, A. E., Pisani, N. T. & Siongok, T. A. (1979). A new method for the purification of human eosinophils and neutrophils, and a comparison of the ability of these cells to damage schistosomula of Schistosoma mansoni. Journal of immunology 122, 1228–36.Google Scholar
Verwaerde, C., Grzych, J.-M., Bazin, H., Capron, M. & Capron, A. (1979). Production d'anticorps monoclonaux anti Schistosoma mansoni. Etude preliminaire de leurs activities biologiques. Comptes rendus Académie des Sciences, Paris, Serie D 289, 725–7.Google Scholar