Hostname: page-component-586b7cd67f-tf8b9 Total loading time: 0 Render date: 2024-11-26T14:13:07.199Z Has data issue: false hasContentIssue false
  • English
  • Français

Molecular phylogenetic analysis of the genus Gyrodactylus (Platyhelminthes: Monogenea) inferred from rDNA ITS region: subgenera versus species groups

Published online by Cambridge University Press:  05 December 2003

I. MATEJUSOVÁ ,
M. GELNAR ,
O. VERNEAU ,
C. O. CUNNINGHAM  and
D. T. J. LITTLEWOOD
I. MATEJUSOVÁ
Affiliation:
FRS Marine Laboratory, P.O. Box 101, Victoria Road, Aberdeen AB11 9DB, UK
M. GELNAR
Affiliation:
Faculty of Sciences, Masaryk University, Kotlarska 2, 61135 Brno, Czech Republic
O. VERNEAU
Affiliation:
UMR 5555 CNRS, Parasitologie Fonctionnelle et Evolutive, CBETM, Université de Perpignan, A. Paul Alduy, 66860 Perpignan Cedex, France
C. O. CUNNINGHAM
Affiliation:
FRS Marine Laboratory, P.O. Box 101, Victoria Road, Aberdeen AB11 9DB, UK
D. T. J. LITTLEWOOD
Affiliation:
Parasitic Worms Division, Department of Zoology, The Natural History Museum, Cromwell Road, London SW7 5BD, UK
Get access Rights & Permissions [Opens in a new window]

Abstract

Analyses of small subunit ribosomal RNA gene sequences of representatives of major taxa of Monopisthocotylea were performed to identify the sister group of Gyrodactylus. Nuclear ribosomal DNA sequencesNucleotide sequence data reported in this paper are available in the GenBank, DDBJ and EMBL databases under the accession numbers AJ566375-79, AJ566768, AJ567670-74, AJ581657. from the complete internal transcribed spacer (ITS) region were used to infer phylogeny of 37 Gyrodactylus species and Gyrodactyloides bychowskii, Macrogyrodactylus polypteri and Gyrdicotylus gallieni, using maximum likelihood, parsimony and Bayesian inference. The genus Gyrodactylus appeared to be a monophyletic group in all analyses, based on the present data set. Within the genus, there were 3 major groups recognized by high bootstrap values and posterior probabilities. None of the 6 subgenera appeared to be monophyletic, and the most basal subgenus G. (Gyrodactylus) was paraphyletic. Characteristics of the excretory system of Gyrodactylus do not seem to be conservative enough to reveal subgenera within Gyrodactylus and we suggest abandoning existing subgenera as indicators of phylogeny. The grouping of species based on the morphology of the ventral bar and marginal hooks seems to have sufficient power to infer relationships between the Gyrodactylus species.

Keywords

MonogeneaGyrodactylusSSUITSphylogeny
Type
Research Article
Information
Parasitology , Volume 127 , Issue 6 , December 2003 , pp. 603 - 611
Copyright
2003 Cambridge University Press

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

AUDEBERT, F., DURETTE-DESSET, M.-C. & CHILTON, N. B. ( 2000). Internal transcribed spacer rDNA can be used to infer the phylogenetic relationships of species within the genus Nematodirus (Nematoda: Molineoidea). International Journal for Parasitology 30, 187–191.CrossRefGoogle Scholar
BAKKE, T. A., HARRIS, P. D. & CABLE, J. ( 2002). Host specificity dynamics: observations on gyrodactylid monogeneans. International Journal for Parasitology 32, 281–308.CrossRefGoogle Scholar
BENTZ, S., LEROY, S., DUPREEZ, L., MARIAUX, J., VAUCHER, C. & VERNEAU, O. ( 2001). Origin and evolution of African Polystoma (Monogenea: Polystomatidae) assessed by molecular methods. International Journal for Parasitology 31, 697–705.CrossRefGoogle Scholar
CABLE, J., HARRIS, P. D., TINSLEY, R. C. & LAZARUS, C. M. ( 1999). Phylogenetic analysis of Gyrodactylus spp. (Platyhelminthes: Monogenea) using ribosomal DNA sequences. Canadian Journal of Zoology 77, 1439–1449.Google Scholar
CUNNINGHAM, C. O. ( 1997). Species variation within the internal transcribed spacer (ITS) region of Gyrodactylus (Monogenea: Gyrodactylidae) ribosomal RNA genes. Journal of Parasitology 83, 215–219.CrossRefGoogle Scholar
CUNNINGHAM, C. O., McGILLIVRAY, D. M., MACKENZIE, K. & MELVIN, W. T. ( 1995 a). Discrimination between Gyrodactylus salaris, G. derjavini and G. truttae (Platyhelminthes: Monogenea) using restriction fragment length polymorphisms and an oligonucleotide probe within the small subunit ribosomal RNA gene. Parasitology 111, 87–94.Google Scholar
CUNNINGHAM, C. O., McGILLIVRAY, D. M. & MACKENZIE, K. ( 1995 b). Phylogenetic analysis of Gyrodactylus salaris Malmberg, 1957 based on the small subunit (18S) ribosomal RNA gene. Molecular and Biochemical Parasitology 71, 139–142.Google Scholar
CUNNINGHAM, C. O., MO, T. A., COLLINS, C. M., BUCHMANN, K., THIERY, R., BLANC, G. & LAUTRAITE, A. ( 2001). Redescription of Gyrodactylus teuchis Lautraite, Blanc, Thiery, Daniel & Vigneulle, 1999 (Monogenea: Gyrodactylidae), a species identified by ribosomal RNA sequence. Systematic Parasitology 48, 141–150.CrossRefGoogle Scholar
ERGENS, R. ( 1966). Revision of the helminthofauna of fishes from Czechoslovakia IV. Folia Parasitologica, 13, 212–221.Google Scholar
ERGENS, R. ( 1976). Gyrodactylus barbi sp. n. (Monogenoidea) from the fins of barbels. Vestnik Ceskoslovenske Spolecnosti Zoologicke 40, 1961–1962.Google Scholar
ERGENS, R. ( 1980). On the problem of three species of the genus Gyrodactylus, members of “G. wageneri-group” (Gyrodactylidae: Monogenea). Helminthologia 17, 257–267.Google Scholar
ERGENS, R. & BYCHOWSKY, B. E. ( 1967). Revision of the species Gyrodactylus nemachili Bychowsky, 1936 (Monogenoidea). Folia Parasitologica 14, 225–238.Google Scholar
ERGENS, R. & YUKHIMENKO, S. S. ( 1975). Gyrodactylus (Monogenoidea) from some Rhodeinae (Cypriniformes). Folia Parasitologica 22, 33–36.Google Scholar
ERNST, I., FLETCHER, A. & HAYWARD, C. ( 2000). Gyrodactylus anguillae (Monogenea: Gyrodactylidae) from anguilid eels (Anguilla australis and Anguilla reinhardtii) in Australia: a native or an exotic? Journal of Parasitology 86, 1152–1156.Google Scholar
FARRIS, J. S., KÄLLERSJO, M., KLUGE, A. G. & BULT, C. ( 1994). Testing significance of incongruence. Cladistics 10, 315–319.CrossRefGoogle Scholar
GLÄSER, H.-J. ( 1974 a). Sechs neue Arten der Gyrodactylus-wageneri Gruppe (Monogenea, Gyrodactylidae) nebst Bemerkungen zur Preparation, Determination, Terminologie und Wirtsspezifität. Zoologischer Anzeiger 192, 56–76.Google Scholar
GLÄSER, H.-J. ( 1974 b). Eine neue Artengruppe des Subgenus Gyrodactylus (Paranephrotus) (Monogenea, Gyrodactylidae). Zoologischer Anzeiger 192, 271–278.Google Scholar
HARRIS, P. D. ( 1985). Species of Gyrodactylus von Nordmann, 1832 (Monogenea: Gyrodactylidae) from freshwater fishes in southern England, with a description of Gyrodactylus rogatensis sp. Nov. from the bullhead Cottus gobio L. Journal of Natural History 19, 791–809.Google Scholar
HARRIS, P. D. ( 1986). Species of Gyrodactylus von Nordmann, 1832 (Monogenea, Gyrodactylidae) from poeciliid fishes, with a description of G. turnbulli sp., nov. from the guppy, Poecilia reticulata Peters. Journal of Natural History 20, 183–191.Google Scholar
HARRIS, P. D. & CABLE, J. ( 2000). Gyrodactylus poeciliae n. sp. and G. milleri n. sp. (Monogenea: Gyrodactylidae) from Poecilia caucana (Steindachner) in Venezuela. Systematic Parasitology 47, 79–85.Google Scholar
HUELSENBECK, J. P. & RONQUIST, F. ( 2001). MrBayes: Bayesian inference of phylogenetic trees. Bioinformatics 17, 754–755.CrossRefGoogle Scholar
HUELSENBECK, J. P., RONQUIST, F., NIELSEN, R. & BOLLBACK, J. P. ( 2001). Bayesian inference of phylogeny and its impact on evolutionary biology. Science 294, 2310–2314.CrossRefGoogle Scholar
HUYSE, T. & VOLCKAERT, F. A. M. ( 2002). Identification of a host-associated species complex using molecular and morphometric analyses, with the description of Gyrodactylus rugiensoides n. sp. (Gyrodactylidae, Monogenea). International Journal for Parasitology 32, 907–919.CrossRefGoogle Scholar
JEANMOUGIN, F., THOMPSON, J. D., GOUY, M., HIGGINS, D. G. & GIBSON, T. J. ( 1998). Multiple sequence alignment with Clustal X. Trends in Biochemical Sciences 23, 403–405.CrossRefGoogle Scholar
MADDISON, W. P. & MADDISON, D. R. ( 2000). MacClade. Version 4. Sinauer Associates, Sunderland, Massachusetts.
MALMBERG, G. ( 1956). Om förekomsten av Gyrodactylus på svenska fiskar. Skrifter utgivna av Södra Sveriges Fiskeriförening, Årsskrift, pp. 19– 76.
MALMBERG, G. ( 1964). Taxonomical and ecological problems in Gyrodactylus (Trematoda, Monogenea). In Parasitic Worms and Aquatic Conditions ( ed. Ergens, R. & Rysavy, B. ), pp. 203–230. Czech Academy of Science, Prague, Czech Republic.
MALMBERG, G. ( 1970). The excretory systems and the marginal hooks as a basis for the systematics of Gyrodactylus (Trematoda, Monogenea). Arkiv för Zoologi, Serie 2, Band, 23 (1). Almqvist & Wiksell, Stockholm.
MALMBERG, G. ( 1998). On the evolution within the family Gyrodactylidae (Monogenea). International Journal for Parasitology 28, 1625–1635.CrossRefGoogle Scholar
MATEJUSOVA, I., GELNAR, M., McBEATH, A. J. A., COLLINS, C. M. & CUNNINGHAM, C. O. ( 2001). Molecular markers for gyrodactylids (Gyrodactylidae: Monogenea) from five fish families (Teleostei). International Journal for Parasitology 31, 738–745.CrossRefGoogle Scholar
OLSON, P. D. & LITTLEWOOD, D. T. J. ( 2002). Phylogenetics of the Monogenea – evidence from a medley of molecules. International Journal for Parasitology 32, 233–244.CrossRefGoogle Scholar
POSADA, D. & CRANDALL, K. A. ( 1998). Modeltest: testing the model of DNA substitution. Bioinformatics 14, 817–818.CrossRefGoogle Scholar
STEANE, D. A., McKINNON, G. E., VAILLANCOURT, R. E. & POTTS, B. M. ( 1999). ITS sequence data resolve higher level relationships among the eucalypts. Molecular Phylogenetics and Evolution 12, 215–223.CrossRefGoogle Scholar
SWOFFORD, D. L. ( 2002). PAUP*. Phylogenetic Analysis Using Parsimony (*and Other Methods). 4th Edn. Sinauer Associates, Sunderland, Massachusetts.
WILLIAMS, H. & JONES, A. ( 1994). Parasitic worms of fish. Taylor and Francis, London.CrossRef
ZIETARA, M. S., ARNDT, A., GEETS, A., HELLEMANS, B. & VOLCKAERT, F. A. M. ( 2000). The nuclear rDNA region of Gyrodactylus arcuatus and G. branchicus (Monogenea: Gyrodactylidae). Journal of Parasitology 86, 1368–1373.Google Scholar
ZIETARA, M. S., HUYSE, T., LUMME, J. & VOLCKAERT, F. A. M. ( 2002). Deep divergence among subgenera of Gyrodactylus inferred from rDNA ITS region. Parasitology 124, 39–52.CrossRefGoogle Scholar
ZIETARA, M. S. & LUMME, J. ( 2002). Speciation by host switch and adaptive radiation in a fish parasite genus Gyrodactylus (Monogenea, Gyrodactylidae). Evolution 56, 2445–2458.CrossRefGoogle Scholar
ZITNAN, R. ( 1964). Gyrodactylus rhodei sp. n. – a new monogenean species from skin of Rhodeus sericeus amarus (Bloch). Helminthologia 5 , 1–4.Google Scholar