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Molecular determination of the origin of acephalic cysticercus

Published online by Cambridge University Press:  25 November 2004

J.-Y. CHUNG ,
W.-G. KHO ,
S.-Y. HWANG ,
E.-Y. JE ,
Y.-T. CHUNG ,
T.-S. KIM ,
K.-S. EOM ,
W.-M. SOHN ,
S.-Y. CHO  and
Y. KONG
J.-Y. CHUNG
Affiliation:
Department of Parasitology, College of Medicine and Department of Malariology, Paik Institute for Clinical Research, Inje University, Busan 614-735, Korea
W.-G. KHO
Affiliation:
Department of Parasitology, College of Medicine and Department of Malariology, Paik Institute for Clinical Research, Inje University, Busan 614-735, Korea
S.-Y. HWANG
Affiliation:
Department of Parasitology, College of Medicine and Department of Malariology, Paik Institute for Clinical Research, Inje University, Busan 614-735, Korea
E.-Y. JE
Affiliation:
Department of Molecular Parasitology and Center for Molecular Medicine, Sungkyunkwan University School of Medicine and Samsung Biomedical Research Institute, Suwon, 440-746, Korea
Y.-T. CHUNG
Affiliation:
Department of Neurosurgery, Inje University College of Medicine, Busan 614-735, Korea
T.-S. KIM
Affiliation:
Department of Tropical and Endemic Parasitic Diseases, National Institute of Health, Seoul 122-701, Korea
K.-S. EOM
Affiliation:
Department of Parasitology, Chungbuk National University College of Medicine, Chongju 360-763, Korea
W.-M. SOHN
Affiliation:
Department of Parasitology and Institute of Health Sciences, Gyeongsang National University College of Medicine, Jinju 660-751, Korea
S.-Y. CHO
Affiliation:
Department of Molecular Parasitology and Center for Molecular Medicine, Sungkyunkwan University School of Medicine and Samsung Biomedical Research Institute, Suwon, 440-746, Korea
Y. KONG
Affiliation:
Department of Molecular Parasitology and Center for Molecular Medicine, Sungkyunkwan University School of Medicine and Samsung Biomedical Research Institute, Suwon, 440-746, Korea
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Abstract

Acephalic cysticercus (Ac), a rarely developed multilobulated and nonencysted form of larval Taenia, causes hydrocephalus or adhesive arachnoiditis in the ventricles and subarachnoidal space that often lead to fatal outcome in affected patients. Ac has been proposed to originate from T. solium on the basis of morphological features, while no molecular data supporting the presumption have been available. In the present study, we investigated the immunological properties as well as molecular characteristics of Ac that was obtained surgically from 6 patients. Immunoblotting of the cyst fluid from Ac samples demonstrated the constitutive expression of a T. solium metacestode (TsM) 10 kDa protein. Specific antibodies against the truncated 10 kDa protein, which appears to be species specific for TsM cysticercosis, were detected in both serum and cerebrospinal fluid samples of Ac patients. Nucleotide sequences of mitochondrial cytochrome c oxidase subunit I (COI) and NADH dehydrogenase subunit 1 (ND1) genes of Ac were almost identical to those of T. solium but differed substantially from those of the other Taenia species. In phylogenetic analysis, Ac clustered with T. solium in a well-supported clade. Our results strongly suggest that Ac may have originated from T. solium.

Keywords

acephalic cysticercusneurocysticercosis10 kDa proteincytochrome c oxidase INADH dehydrogenase subunit 1
Type
Research Article
Information
Parasitology , Volume 130 , Issue 2 , February 2005 , pp. 239 - 246
Copyright
© 2005 Cambridge University Press

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